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J. Biol. Chem., Vol. 277, Issue 52, 50959-50965, December 27, 2002
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From the Departments of Probiotic bacteria are microorganisms that
benefit the host by preventing or ameliorating disease. However,
little information is known regarding the scientific rationale for
using probiotics as alternative medicine. The purpose of this paper is
to investigate the mechanisms of probiotic beneficial effects on
intestinal cell homeostasis. We now report that one such probiotic,
Lactobacillus rhamnosus GG (LGG), prevents cytokine-induced
apoptosis in two different intestinal epithelial cell models. Culture
of LGG with either mouse or human colon cells activates the
anti-apoptotic Akt/protein kinase B. This model probiotic also inhibits
activation of the pro-apoptotic p38/mitogen-activated protein kinase by
tumor necrosis factor, interleukin-1
Probiotic Bacterium Prevents Cytokine-induced Apoptosis in
Intestinal Epithelial Cells*
and§¶
Pediatrics and
§ Cell and Developmental Biology, Division of
Gastroenterology, Hepatology, and Nutrition, Vanderbilt University
School of Medicine, Nashville, Tennessee 37232
, or 
-interferon.
Furthermore, products recovered from LGG culture broth supernatant show
concentration-dependent activation of Akt and inhibition of
cytokine-induced apoptosis. These observations suggest a novel
mechanism of communication between probiotic microorganisms and
epithelia that increases survival of intestinal cells normally found in
an environment of pro-apoptotic cytokines.
*
This work was supported by a Children's Digestive
Health and Nutrition Foundation/Nestle Nutrition Grant (to F. Y.),
National Institutes of Health Grants DK10105 (to F. Y.) and DK56008
(to D. B. P.), and the Vanderbilt University Digestive Disease
Research Center (DK58404).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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