JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M106587200 on November 28, 2001

J. Biol. Chem., Vol. 277, Issue 6, 3979-3984, February 8, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/6/3979    most recent
M106587200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Uchimura, K.
Right arrow Articles by Muramatsu, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uchimura, K.
Right arrow Articles by Muramatsu, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Specificities of N-Acetylglucosamine-6-O-sulfotransferases in Relation to L-selectin Ligand Synthesis and Tumor-associated Enzyme Expression*

Kenji UchimuraDagger §, Fathy M. El-FasakhanyDagger , Mayuko HoriDagger , Stefan Hemmerich||, Sarah E. Blink**, Geoffrey S. Kansas**Dagger Dagger , Akiko Kanamori§§, Kensuke Kumamoto§§, Reiji Kannagi§§, and Takashi MuramatsuDagger ¶¶

From the Dagger  Department of Biochemistry, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan, || Thios Biotechnology, Oakland, California 94609, the ** Department of Microbiology-Immunology, Northwestern University School of Medicine, Chicago, Illinois 60611-3008, and the §§ Program of Molecular Pathology, Aichi Cancer Center, Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan

N-Acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST) catalyzes the transfer of sulfate from adenosine 3'-phosphate,5'-phosphosulfate to the C-6 position of the non-reducing GlcNAc. Three human GlcNAc6STs, namely GlcNAc6ST-1, GlcNAc6ST-2 (HEC-GlcNAc6ST), and GlcNAc6ST-3 (I-GlcNAc6ST), were produced as fusion proteins to protein A, and their substrate specificities as well as their enzymological properties were determined. Both GlcNAc6ST-1 and GlcNAc6ST-2 efficiently utilized the following oligosaccharide structures as acceptors: GlcNAcbeta 1-6[Galbeta 1-3]GalNAc-pNP (core 2), GlcNAcbeta 1-6ManOMe, and GlcNAcbeta 1-2Man. The ratios of activities to these substrates were not significantly different between the two enzymes. However, GlcNAc6ST-2 but not GlcNAc6ST-1 acted on core 3 of GlcNAcbeta 1-3GalNAc-pNP. GlcNAc6ST-3 used only the core 2 structure among the above mentioned oligosaccharide structures. The ability of GlcNAc6ST-1 to sulfate core 2 structure as efficiently as GlcNAc6ST-2 is consistent with the view that GlcNAc6ST-1 is also involved in the synthesis of L-selectin ligand. Indeed, cells doubly transfected with GlcNAc6ST-1 and fucosyltransferase VII cDNAs supported the rolling of L-selectin-expressing cells. The activity of GlcNAc6ST-2 on core 3 and its expression in mucinous adenocarcinoma suggested that this enzyme corresponds to the sulfotransferase, which is specifically expressed in mucinous adenocarcinoma (Seko, A., Sumiya, J., Yonezawa, S., Nagata, K., and Yamashita, K. (2000) Glycobiology 10, 919-929).

* This work was supported by Grants-in-aid 12003898 and 12480187 for Scientific Research from the Japan Society for the Promotion of Science (to K. U. and T. M.), Grant-in-aid 10178102 for Scientific Research on Priority Areas from the Ministry of Education, Science, Culture, and Sports of Japan (to T. M.), and National Institutes of Health Grant HL55647 (to G. S. K.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Research Fellow of the Japan Society for the Promotion of Science.

Currently taking a leave of absence from Tanta University, Tanta, Egypt.

Dagger Dagger Established Investigator of the American Heart Association.

¶¶ To whom correspondence should be addressed. Tel.: 81-52-744-2059; Fax: 81-52-744-2065; E-mail: tmurama@med.nagoya-u.ac.jp.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N. Kimura, K. Ohmori, K. Miyazaki, M. Izawa, Y. Matsuzaki, Y. Yasuda, H. Takematsu, Y. Kozutsumi, A. Moriyama, and R. Kannagi
Human B-lymphocytes Express {alpha}2-6-Sialylated 6-Sulfo-N-acetyllactosamine Serving as a Preferred Ligand for CD22/Siglec-2
J. Biol. Chem., November 2, 2007; 282(44): 32200 - 32207.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
H. Zhang, T. Muramatsu, A. Murase, S. Yuasa, K. Uchimura, and K. Kadomatsu
N-Acetylglucosamine 6-O-sulfotransferase-1 is required for brain keratan sulfate biosynthesis and glial scar formation after brain injury
Glycobiology, August 1, 2006; 16(8): 702 - 710.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kamiyama, N. Sasaki, E. Goda, K. Ui-Tei, K. Saigo, H. Narimatsu, Y. Jigami, R. Kannagi, T. Irimura, and S. Nishihara
Molecular Cloning and Characterization of a Novel 3'-Phosphoadenosine 5'-Phosphosulfate Transporter, PAPST2
J. Biol. Chem., April 21, 2006; 281(16): 10945 - 10953.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Ohmori, F. Fukui, M. Kiso, T. Imai, O. Yoshie, H. Hasegawa, K. Matsushima, and R. Kannagi
Identification of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis X, a selectin ligand expressed on a subset of skin-homing helper memory T cells
Blood, April 15, 2006; 107(8): 3197 - 3204.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
H. Yagi, N. Takahashi, Y. Yamaguchi, N. Kimura, K. Uchimura, R. Kannagi, and K. Kato
Development of structural analysis of sulfated N-glycans by multidimensional high performance liquid chromatography mapping methods
Glycobiology, October 1, 2005; 15(10): 1051 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
S. L. Tjew, K. L. Brown, R. Kannagi, and P. Johnson
Expression of N-acetylglucosamine 6-O-sulfotransferases (GlcNAc6STs)-1 and -4 in human monocytes: GlcNAc6ST-1 is implicated in the generation of the 6-sulfo N-acetyllactosamine/Lewis x epitope on CD44 and is induced by TNF-{alpha}
Glycobiology, July 1, 2005; 15(7): 7C - 13C.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. L. de Graffenried, S. T. Laughlin, J. J. Kohler, and C. R. Bertozzi
From the Cover: A small-molecule switch for Golgi sulfotransferases
PNAS, November 30, 2004; 101(48): 16715 - 16720.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
L. Chen, K. Ichihara-Tanaka, and T. Muramatsu
Role of the Carboxyl-Terminal Region in the Activity of N-Acetylglucosamine 6-O-Sulfotransferase-1
J. Biochem., November 1, 2004; 136(5): 659 - 664.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. L. de Graffenried and C. R. Bertozzi
The Stem Region of the Sulfotransferase GlcNAc6ST-1 Is a Determinant of Substrate Specificity
J. Biol. Chem., September 17, 2004; 279(38): 40035 - 40043.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Uchimura, K. Kadomatsu, F. M. El-Fasakhany, M. S. Singer, M. Izawa, R. Kannagi, N. Takeda, S. D. Rosen, and T. Muramatsu
N-Acetylglucosamine 6-O-Sulfotransferase-1 Regulates Expression of L-Selectin Ligands and Lymphocyte Homing
J. Biol. Chem., August 13, 2004; 279(33): 35001 - 35008.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Hiraoka, H. Kawashima, B. Petryniak, J. Nakayama, J. Mitoma, J. D. Marth, J. B. Lowe, and M. Fukuda
Core 2 Branching {beta}1,6-N-Acetylglucosaminyltransferase and High Endothelial Venule-restricted Sulfotransferase Collaboratively Control Lymphocyte Homing
J. Biol. Chem., January 23, 2004; 279(4): 3058 - 3067.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. L. de Graffenried and C. R. Bertozzi
Golgi Localization of Carbohydrate Sulfotransferases Is a Determinant of L-selectin Ligand Biosynthesis
J. Biol. Chem., October 10, 2003; 278(41): 40282 - 40295.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Leppanen, T. Yago, V. I. Otto, R. P. McEver, and R. D. Cummings
Model Glycosulfopeptides from P-selectin Glycoprotein Ligand-1 Require Tyrosine Sulfation and a Core 2-branched O-Glycan to Bind to L-selectin
J. Biol. Chem., July 11, 2003; 278(29): 26391 - 26400.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
J. K. Lee, A. Bistrup, A. Van Zante, and S. D. Rosen
Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications
Glycobiology, April 1, 2003; 13(4): 245 - 254.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Seko, N. Dohmae, K. Takio, and K. Yamashita
beta 1,4-Galactosyltransferase (beta 4GalT)-IV Is Specific for GlcNAc 6-O-Sulfate. beta 4GalT-IV ACTS ON KERATAN SULFATE-RELATED GLYCANS AND A PRECURSOR GLYCAN OF 6-SULFOSIALYL-LEWIS X
J. Biol. Chem., March 7, 2003; 278(11): 9150 - 9158.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
F. M. El-Fasakhany, K. Ichihara-Tanaka, K. Uchimura, and T. Muramatsu
N-Acetylglucosamine-6-O-Sulfotransferase-1: Production in the Baculovirus System and Its Applications to the Synthesis of a Sulfated Oligosaccharide and to the Modification of Oligosaccharides in Fibrinogen
J. Biochem., March 1, 2003; 133(3): 287 - 293.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. O. Akama, A. K. Misra, O. Hindsgaul, and M. N. Fukuda
Enzymatic Synthesis in Vitro of the Disulfated Disaccharide Unit of Corneal Keratan Sulfate
J. Biol. Chem., November 1, 2002; 277(45): 42505 - 42513.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kanamori, N. Kojima, K. Uchimura, T. Muramatsu, T. Tamatani, M. C. Berndt, G. S. Kansas, and R. Kannagi
Distinct Sulfation Requirements of Selectins Disclosed Using Cells That Support Rolling Mediated by All Three Selectins under Shear Flow. L-SELECTIN PREFERS CARBOHYDRATE 6-SULFATION TO TYROSINE SULFATION, WHEREAS P-SELECTIN DOES NOT
J. Biol. Chem., August 30, 2002; 277(36): 32578 - 32586.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
A. Seko, K. Nagata, S. Yonezawa, and K. Yamashita
Ectopic expression of a GlcNAc 6-O-sulfotransferase, GlcNAc6ST-2, in colonic mucinous adenocarcinoma
Glycobiology, June 1, 2002; 12(6): 379 - 388.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.