|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 277, Issue 7, 4738-4746, February 15, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The importance of zinc in organisms is
clearly established, and mechanisms involved in zinc acquisition by
plants have recently received increased interest. In this report, the
identification, characterization and location of GmZIP1, the first
soybean member of the ZIP family of metal transporters, are described.
GmZIP1 was found to possess eight putative transmembrane domains
together with a histidine-rich extra-membrane loop. By functional
complementation of zrt1zrt2 yeast cells no longer able to
take up zinc, GmZIP1 was found to be highly selective for zinc, with an
estimated Km value of 13.8 µM.
Cadmium was the only other metal tested able to inhibit zinc uptake in
yeast. An antibody raised against GmZIP1 specifically localized the
protein to the peribacteroid membrane, an endosymbiotic membrane in
nodules resulting from the interaction of the plant with its
microsymbiont. The specific expression of GmZIP1 in nodules
was confirmed by Northern blot, with no expression in roots, stems, or
leaves of nodulated soybean plants. Antibodies to GmZIP1 inhibited zinc
uptake by symbiosomes, indicating that at least some of the zinc uptake
observed in isolated symbiosomes could be attributed to GmZIP1. The
orientation of the protein in the membrane and its possible role in the
symbiosis are discussed.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY029321.
GmZIP1 Encodes a Symbiosis-specific Zinc Transporter in
Soybean*
§,
,,,, and Laboratoire de Biologie
Végétale et Microbiologie, CNRS FRE 2294, Université
de Nice-Sophia Antipolis, Parc Valrose, 06108 Nice cédex 2, France, the ¶ Biochemistry Department, University of Western
Australia, Crawley, WA 6009, Australia, ** Environmental
Biology, Research School of Biological Sciences, Australian
National University, GPO Box 475, Canberra, Australian Capital
Territory 2601, Australia, §§ Department of
Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755, and Max Planck Institute of Molecular Plant
Physiology, am Mühlenberg 1, 14476 Golm, Germany
*
This research was supported by the Australian Research
Council (to D. A. D.), the CNRS Programme International de
Cooperation Scientifique Program 637 (to S. M., A. P.), and the
Department of Energy Grant DE-FG07-97ER20292 (to M. L. G.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by an Australian Postgraduate Research Award.
¶¶
To whom correspondence should be addressed. Tel.:
61-8-93803325; Fax: 61-8-93801148; E-mail:
dday@cyllene.uwa.edu.au.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. White, J. Prell, E. K. James, and P. Poole Nutrient Sharing between Symbionts Plant Physiology, June 1, 2007; 144(2): 604 - 614. [Full Text] [PDF]
|
|
|
|
|
|
M. J. Haydon and C. S. Cobbett A Novel Major Facilitator Superfamily Protein at the Tonoplast Influences Zinc Tolerance and Accumulation in Arabidopsis Plant Physiology, April 1, 2007; 143(4): 1705 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Krusell, K. Krause, T. Ott, G. Desbrosses, U. Kramer, S. Sato, Y. Nakamura, S. Tabata, E. K. James, N. Sandal, et al. The Sulfate Transporter SST1 Is Crucial for Symbiotic Nitrogen Fixation in Lotus japonicus Root Nodules PLANT CELL, May 1, 2005; 17(5): 1625 - 1636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Vincill, K. Szczyglowski, and D. M. Roberts GmN70 and LjN70. Anion Transporters of the Symbiosome Membrane of Nodules with a Transport Preference for Nitrate Plant Physiology, April 1, 2005; 137(4): 1435 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yazaki, Z. Shimatani, A. Hashimoto, Y. Nagata, F. Fujii, K. Kojima, K. Suzuki, T. Taya, M. Tonouchi, C. Nelson, et al. Transcriptional profiling of genes responsive to abscisic acid and gibberellin in rice: phenotyping and comparative analysis between rice and Arabidopsis Physiol Genomics, July 15, 2004; 17(2): 87 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jeong, S. Suh, C. Guan, Y.-F. Tsay, N. Moran, C. J. Oh, C. S. An, K. N. Demchenko, K. Pawlowski, and Y. Lee A Nodule-Specific Dicarboxylate Transporter from Alder Is a Member of the Peptide Transporter Family Plant Physiology, March 1, 2004; 134(3): 969 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hall and L. E. Williams Transition metal transporters in plants J. Exp. Bot., December 1, 2003; 54(393): 2601 - 2613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Ramesh, R. Shin, D. J. Eide, and D. P. Schachtman Differential Metal Selectivity and Gene Expression of Two Zinc Transporters from Rice Plant Physiology, September 1, 2003; 133(1): 126 - 134. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |