JBC Oz Biosciences

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M108623200 on November 12, 2001

J. Biol. Chem., Vol. 277, Issue 7, 5322-5329, February 15, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/7/5322    most recent
M108623200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boudreau, R. T. M.
Right arrow Articles by Lin, T.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boudreau, R. T. M.
Right arrow Articles by Lin, T.-J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Protein Phosphatase 2A and Protein Kinase Calpha Are Physically Associated and Are Involved in Pseudomonas aeruginosa-induced Interleukin 6 Production by Mast Cells*

Robert T. M. BoudreauDagger , Rafael GardunoDagger §, and Tong-Jun LinDagger ||

From the Departments of Dagger  Microbiology and Immunology, § Medicine, and  Pediatrics, Dalhousie University, Halifax, Nova Scotia B3J 3G9, Canada

Pulmonary infection with Pseudomonas aeruginosa is characterized by massive airway inflammation, which comprises significant cytokine production. Although mast cells are abundant in the lung and are potent sources of various cytokines, a role of mast cells in P. aeruginosa infection remains undefined, and P. aeruginosa-induced signaling mechanisms in mast cells have not been studied previously. Here we demonstrate that human cord blood-derived mast cells, mouse bone marrow-derived mast cells, and the mouse mast cell line MC/9 produce significant amounts of interleukin 6 (IL-6) in response to P. aeruginosa. This response was accompanied by a stimulation of protein kinase Calpha (PKCalpha ) phosphorylation and PKC activity and was significantly blocked by the PKC inhibitors Ro 31-8220 and PKCalpha pseudosubstrate. Interestingly, mast cells treated with P. aeruginosa had reduced protein levels of phosphatase 2A catalytic unit (PP2Ac), which prompted us to determine whether a direct association between PKCalpha and PP2A occurs in mast cells. In mouse bone marrow-derived mast cells and MC/9 cells, as well as in the human mast cell line HMC-1, PP2A coimmunoprecipitated with PKCalpha either using PKCalpha - or PP2Ac-specific antibodies, suggesting that PKCalpha and PP2Ac are physically associated in mast cells. The PP2A inhibitor okadaic acid induced P. aeruginosa-like responses in mast cells including increased PKCalpha phosphorylation, stimulated PKC activity, and augmented IL-6 production, the last being blocked by the PKC inhibitor Ro 31-8220. Finally, okadaic acid potentiated the P. aeruginosa-induced IL-6 production. Collectively, these data provide, to our knowledge, the first evidence of both a direct physical association of PP2A and PKCalpha in mammalian cells and their coinvolvement in regulating mast cell activation in response to P. aeruginosa.

* This work was supported in part by grants from Canadian Institutes of Health Research, Canadian Cystic Fibrosis Foundation, Nova Scotia Health Research Foundation, and the Izaak Walton Killam (IWK) Health Center.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| Supported by an IWK Health Center investigatorship. To whom correspondence should be addressed: IWK Health Center, Dept. of Pediatrics, 5850 University Ave., Halifax, NS B3J 3G9, Canada. Fax: 902-428-3217; E-mail: tlin@is.dal.ca.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
Y. Hai, W. Cao, G. Liu, S.-P. Hong, S. A. Elela, R. Klinck, J. Chu, and J. Xie
A G-tract element in apoptotic agents-induced alternative splicing
Nucleic Acids Res., June 1, 2008; 36(10): 3320 - 3331.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. H. Lee, H. J. Lim, S. Yoon, J. K. Seong, D. S. Bae, S. G. Rhee, and Y. S. Bae
Ahnak Protein Activates Protein Kinase C (PKC) through Dissociation of the PKC-Protein Phosphatase 2A Complex
J. Biol. Chem., March 7, 2008; 283(10): 6312 - 6320.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M.-A. Meilleur, C. D. Akpovi, R.-M. Pelletier, and M. L. Vitale
Tumor Necrosis Factor-{alpha}-Induced Anterior Pituitary Folliculostellate TtT/GF Cell Uncoupling Is Mediated by Connexin 43 Dephosphorylation
Endocrinology, December 1, 2007; 148(12): 5913 - 5924.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. E. Jenkins, A. Swiatoniowski, M. R. Power, and T.-J. Lin
Pseudomonas aeruginosa-Induced Human Mast Cell Apoptosis Is Associated with Up-Regulation of Endogenous Bcl-xS and Down-Regulation of Bcl-xL
J. Immunol., December 1, 2006; 177(11): 8000 - 8007.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. A. Kiely, D. O'Gorman, K. Luong, D. Ron, and R. O'Connor
Insulin-Like Growth Factor I Controls a Mutually Exclusive Association of RACK1 with Protein Phosphatase 2A and {beta}1 Integrin To Promote Cell Migration.
Mol. Cell. Biol., June 1, 2006; 26(11): 4041 - 4051.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Li, M. R. Power, and T.-J. Lin
De novo synthesis of early growth response factor-1 is required for the full responsiveness of mast cells to produce TNF and IL-13 by IgE and antigen stimulation
Blood, April 1, 2006; 107(7): 2814 - 2820.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. J. Crossthwaite, A. Ciruela, T. F. Rayner, and D. M. F. Cooper
A Direct Interaction between the N Terminus of Adenylyl Cyclase AC8 and the Catalytic Subunit of Protein Phosphatase 2A
Mol. Pharmacol., February 1, 2006; 69(2): 608 - 617.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Peng, M. R. Power, B. Li, and T.-J. Lin
Inhibition of IKK down-regulates antigen + IgE-induced TNF production by mast cells: a role for the IKK-I{kappa}B-NF-{kappa}B pathway in IgE-dependent mast cell activation
J. Leukoc. Biol., June 1, 2005; 77(6): 975 - 983.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
R. T. M. Boudreau, D. W. Hoskin, and T.-J. Lin
Phosphatase inhibition potentiates IL-6 production by mast cells in response to Fc{varepsilon}RI-mediated activation: involvement of p38 MAPK
J. Leukoc. Biol., November 1, 2004; 76(5): 1075 - 1081.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. E. Jenkins, A. Swiatoniowski, A. C. Issekutz, and T.-J. Lin
Pseudomonas aeruginosa Exotoxin A Induces Human Mast Cell Apoptosis by a Caspase-8 and -3-dependent Mechanism
J. Biol. Chem., August 27, 2004; 279(35): 37201 - 37207.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Anderson, P. K. Noeldner, K. Reece, B. E. Wadzinski, and A. R. Means
Regulation and Function of the Calcium/Calmodulin-dependent Protein Kinase IV/Protein Serine/Threonine Phosphatase 2A Signaling Complex
J. Biol. Chem., July 23, 2004; 279(30): 31708 - 31716.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. Lenasi, K. Kohlstedt, B. Fichtlscherer, A. Mulsch, R. Busse, and I. Fleming
Amlodipine activates the endothelial nitric oxide synthase by altering phosphorylation on Ser1177 and Thr495
Cardiovasc Res, October 1, 2003; 59(4): 844 - 853.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T.-J. Lin, L. H. Maher, K. Gomi, J. D. McCurdy, R. Garduno, and J. S. Marshall
Selective Early Production of CCL20, or Macrophage Inflammatory Protein 3{alpha}, by Human Mast Cells in Response to Pseudomonas aeruginosa
Infect. Immun., January 1, 2003; 71(1): 365 - 373.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T.-J. Lin, R. Garduno, R. T. M. Boudreau, and A. C. Issekutz
Pseudomonas aeruginosa Activates Human Mast Cells to Induce Neutrophil Transendothelial Migration Via Mast Cell-Derived IL-1{alpha} and {beta}
J. Immunol., October 15, 2002; 169(8): 4522 - 4530.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.