JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M108316200 on November 15, 2001

J. Biol. Chem., Vol. 277, Issue 7, 5660-5666, February 15, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
277/7/5660    most recent
M108316200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanguy Le Gac, N.
Right arrow Articles by Boehmer, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanguy Le Gac, N.
Right arrow Articles by Boehmer, P. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Activation of the Herpes Simplex Virus Type-1 Origin-binding Protein (UL9) by Heat Shock Proteins*

Nicolas Tanguy Le Gac and Paul E. BoehmerDagger

From the Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, Florida 33101-6129

Heat shock proteins participate in the initiation of DNA replication of different organisms by facilitating the assembly of initiation complexes. We have examined the effects of human heat shock proteins (Hsp40 and Hsp70) on the interaction of the herpes simplex virus type-1 initiator protein (UL9) with oriS, one of the viral origins of replication. Hsp40 and Hsp70 act substoichiometrically to increase the affinity of UL9 for oriS. The major contributor to this effect is Hsp40. Heat shock proteins also stimulate the ATPase activity of UL9 with oriS and increase opening of the origin. In contrast, heat shock proteins have no effect on the origin-independent activities of UL9 suggesting that their role is not merely in refolding denatured protein. These observations are consistent with a role for heat shock proteins in activating UL9 to efficiently initiate viral origin-dependent DNA replication. The action of heat shock proteins in this capacity is analogous to their role in activating the initiator proteins of other organisms.

* This work was supported by National Institutes of Health Grant GM62643 and American Heart Association Grant 0050973B.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, University of Miami School of Medicine, P.O. Box 016129, Miami, FL 33101-6129. Tel.: 305-243-2934; Fax: 305-243-3955; E-mail: pboehmer@molbio.med.miami.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
T. Carsillo, Z. Traylor, C. Choi, S. Niewiesk, and M. Oglesbee
hsp72, a Host Determinant of Measles Virus Neurovirulence
J. Virol., November 15, 2006; 80(22): 11031 - 11039.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
O. Schildgen, S. Graper, J. Blumel, and B. Matz
Genome Replication and Progeny Virion Production of Herpes Simplex Virus Type 1 Mutants with Temperature-Sensitive Lesions in the Origin-Binding Protein
J. Virol., June 1, 2005; 79(11): 7273 - 7278.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Trabold, M. Weinberger, L. Feng, and W. C. Burhans
Activation of Budding Yeast Replication Origins and Suppression of Lethal DNA Damage Effects on Origin Function by Ectopic Expression of the Co-chaperone Protein Mge1
J. Biol. Chem., April 1, 2005; 280(13): 12413 - 12421.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Fewell, C. M. Smith, M. A. Lyon, T. P. Dumitrescu, P. Wipf, B. W. Day, and J. L. Brodsky
Small Molecule Modulators of Endogenous and Co-chaperone-stimulated Hsp70 ATPase Activity
J. Biol. Chem., December 3, 2004; 279(49): 51131 - 51140.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Macao, M. Olsson, and P. Elias
Functional Properties of the Herpes Simplex Virus Type I Origin-binding Protein Are Controlled by Precise Interactions with the Activated Form of the Origin of DNA Replication
J. Biol. Chem., July 9, 2004; 279(28): 29211 - 29217.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. S. Trego and D. S. Parris
Functional Interaction between the Herpes Simplex Virus Type 1 Polymerase Processivity Factor and Origin-Binding Proteins: Enhancement of UL9 Helicase Activity
J. Virol., December 1, 2003; 77(23): 12646 - 12659.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. J. Cho, W. J. Yoon, C. H. Moon, S. J. Cha, H. Song, H. R. Cho, S. J. Jang, D. K. Chung, C. S. Jeong, and J. W. Park
Molecular Cloning of a Novel Chaperone-like Protein Induced by Rhabdovirus Infection with Sequence Similarity to the Bacterial Extracellular Solute-binding Protein Family 5
J. Biol. Chem., October 25, 2002; 277(44): 41489 - 41496.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Y. Lin, A. M. Makhov, J. D. Griffith, T. R. Broker, and L. T. Chow
Chaperone Proteins Abrogate Inhibition of the Human Papillomavirus (HPV) E1 Replicative Helicase by the HPV E2 Protein
Mol. Cell. Biol., September 15, 2002; 22(18): 6592 - 6604.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.