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Originally published In Press as doi:10.1074/jbc.C100683200 on January 3, 2002

J. Biol. Chem., Vol. 277, Issue 8, 5715-5718, February 22, 2002
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ACCELERATED PUBLICATION
The Integration of YidC into the Cytoplasmic Membrane of Escherichia coli Requires the Signal Recognition Particle, SecA and SecYEG*

Hans-Georg KochDagger §, Michael MoserDagger , Karl-Ludwig Schimz, and Matthias MüllerDagger

From the Dagger  Institute for Biochemistry and Molecular Biology, University Freiburg, Hermann-Herder-Strabeta e 7, D-79104 Freiburg, Germany and the  Institute for Biotechnology, Forschungszentrum Jülich, D-52425 Jülich, Germany

The integration of the polytopic membrane protein YidC into the inner membrane of Escherichia coli was analyzed employing an in vitro system. Upon integration of in vitro synthesized YidC, a 42-kDa membrane protected fragment was detected, which could be immunoprecipitated with polyclonal anti-YidC antibodies. The occurrence of this fragment is in agreement with the predicted topology of YidC and probably encompasses the first two transmembrane domains and the connecting 320-amino acid-long periplasmic loop. The integration of YidC was strictly dependent on the signal recognition particle and SecA. YidC could not be integrated in the absence of SecY, SecE, or SecG, suggesting that YidC, in contrast to its mitochondrial orthologue Oxa1p, cannot engage a SecYEG-independent protein-conducting channel.

* This work was supported by a grant from the Sonderforschungsbereich 388, by the Fonds der Chemischen Industrie, and by Grant QLK3-CT-1999-00917 from the European Union.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed. Tel.: 49-761-2035250; Fax: 49-761-2035253; E-mail: Hans-Georg.Koch@biochemie. uni-freiburg.de.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


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Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.