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J. Biol. Chem., Vol. 277, Issue 8, 6733-6742, February 22, 2002
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From the Centro de Biología Molecular Severo Ochoa
(CSIC-UAM), Universidad Autónoma, Canto Blanco,
28049 Madrid, Spain
The functional role of the
The Bacillus subtilis Phage
29 Protein p16.7,
Involved in 29 DNA Replication, Is a Membrane-localized
Single-stranded DNA-binding Protein*
,
29-encoded integral
membrane protein p16.7 in phage DNA replication was studied using a
soluble variant, p16.7A, lacking the N-terminal membrane-spanning
domain. Because of the protein-primed mechanism of DNA replication, the bacteriophage 29 replication intermediates contain long stretches of
single-stranded DNA (ssDNA). Protein p16.7A was found to be an
ssDNA-binding protein. In addition, by direct and functional analysis
we show that protein p16.7A binds to the stretches of ssDNA of the
29 DNA replication intermediates. Properties of protein p16.7A were
compared with those of the 29-encoded single-stranded DNA-binding
protein p5. The results obtained show that both proteins have
different, non-overlapping functions. The likely role of p16.7 in
attaching 29 DNA replication intermediates to the membrane of the
infected cell is discussed. Homologues of gene 16.7 are present in
29-related phages, suggesting that the proposed role of p16.7 is
conserved in this family of phages.
*
This investigation was supported by National Institutes of
Health Research Grant 2R01 GM27242-22, Dirección General de
Investigación Científica y Técnica Grant
PB98-0645, European Economic Community Grant ERBFMX CT97 0125, and an
Institutional grant from Fundación Ramón Areces.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Holder of a predoctoral fellowship from Gobierno Vasco.
§
To whom correspondence should be addressed: Tel.: 34 91 397 8435;
Fax: 34 91 397 8490; E-mail: Msalas@cbm.uam.es.
¶
Supported by a postdoctoral fellowship from the Spanish
Ministry of Education and Culture.
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