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J. Biol. Chem., Vol. 277, Issue 9, 7262-7270, March 1, 2002
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From the Departamento de Genética y Microbiología,
Facultad de Biología, Universidad de Murcia,
Murcia 30071, Spain
The light-inducible carB operon
encodes all but one of the structural genes for carotenogenesis in
Myxococcus xanthus. It is transcriptionally controlled by
two proteins expressed from two unlinked genetic loci: CarS from the
light-inducible carQRS operon, and CarA from the
light-independent carA operon. CarA represses transcription
from the carB promoter (PB) in the dark, and
CarS counteracts this on illumination. The CarA sequence revealed a
helix-turn-helix DNA-binding motif of the type found in bacterial MerR
transcriptional factors, whereas CarS contains no known DNA-binding motif. Here, we examine the molecular interplay between CarA and CarS.
We demonstrate the following. (i) Whereas CarS exhibits no DNA binding
in vitro, CarA binds specifically to a region encompassing PB to form at least two distinct complexes. (ii) A
palindrome located between positions
A Repressor-Antirepressor Pair Links Two Loci Controlling
Light-induced Carotenogenesis in Myxococcus xanthus*
§,¶,
, and
46 and 63 relative to the
transcription start point is essential but not sufficient for the
formation of the two CarA-DNA complexes observed. (iii) CarS abrogates
the specific DNA binding of CarA. CarA is therefore a repressor and CarS an antirepressor. (iv) CarS physically interacts with CarA; thus,
the functional interaction between them is mediated by protein-protein interactions.
*
This work was supported in part by Grant BMC2000-1006 from
the Dirección General de Investigación-Ministerio de
Ciencia y Tecnología, Spain (to F. J. M.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Both authors contributed equally to this work.
§
Supported by a fellowship from Fundación Séneca
(Murcia, Spain).
¶
To whom correspondence may be addressed. Tel.: 34-968-367-134;
Fax: 34-968-363-963; E-mail: melias@um.es.
Supported by Ministerio de Ciencia y Tecnología, Spain.
**
To whom correspondence may be addressed. Tel.: 34-968-364-951; Fax:
34-968-363-963; E-mail: araujo@um.es.
Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
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