Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208520200 on October 23, 2002

J. Biol. Chem., Vol. 278, Issue 1, 382-390, January 3, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/1/382    most recent
M208520200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by López-Casillas, F.
Right arrow Articles by Brandan, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by López-Casillas, F.
Right arrow Articles by Brandan, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Betaglycan Expression Is Transcriptionally Up-regulated during Skeletal Muscle Differentiation
CLONING OF MURINE BETAGLYCAN GENE PROMOTER AND ITS MODULATION BY MyoD, RETINOIC ACID, AND TRANSFORMING GROWTH FACTOR-beta *

Fernando López-CasillasDagger §, Cecilia Riquelme, Yoshiaki Pérez-KatoDagger , M. Verónica Ponce-CastañedaDagger , Nelson Osses, José Esparza-LópezDagger , Gerardo González-NúñezDagger , Claudio Cabello-Verrugio, Valentín MendozaDagger , Victor Troncoso, and Enrique Brandan§||

From the Dagger  Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-246, México City, D.F., 04510, México and the  Centro de Regulación Celular y Patología, Facultad de Ciencias Biológicas, Millenium Institute for Fundamental and Applied Biology, P. Universidad Católica de Chile, Casilla 114-D, Santiago, Chile

Betaglycan is a membrane-anchored proteoglycan co-receptor that binds transforming growth factor beta  (TGF-beta ) via its core protein and basic fibroblast growth factor through its glycosaminoglycan chains. In this study we evaluated the expression of betaglycan during the C2C12 skeletal muscle differentiation. Betaglycan expression, as determined by Northern and Western blot, was up-regulated during the conversion of myoblasts to myotubes. The mouse betaglycan gene promoter was cloned, and its sequence showed putative binding sites for SP1, Smad3, Smad4, muscle regulatory factor elements such as MyoD and MEF2, and retinoic acid receptor. Transcriptional activity of the mouse betaglycan promoter reporter was also up-regulated in differentiating C2C12 cells. We found that MyoD, but not myogenin, stimulated this transcriptional activity even in the presence of high serum. Betaglycan promoter activity was increased by RA and inhibited by the three isoforms of TGF-beta . On the other hand, basic fibroblast growth factor, BMP-2, and hepatocyte growth factor/scatter factor, which are inhibitors of myogenesis, had little effect. In myotubes, up-regulated betaglycan was also detectable by TGF-beta affinity labeling and immunofluorescence microscopy studies. The latter indicated that betaglycan was localized both on the cell surface and in the ECM. Forced expression of betaglycan in C2C12 myoblasts increases their responsiveness to TGF-beta 2, suggesting that it performs a TGF-beta presentation function in this cell lineage. These results indicate that betaglycan expression is up-regulated during myogenesis and that MyoD and RA modulate its expression by a mechanism that is independent of myogenin.

* This work was supported in part by FONDAP-Biomedicine Grant 13980001 (to E. B.). The Millenium Institute for Fundamental and Applied Biology is financed in part by the Ministerio de Planificación y Cooperación (Chile).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF537325.

§ Supported in part by International Research Scholars grants from the Howard Hughes Medical Institute.

|| Recipient of a Presidential Chair in Science from the Chilean Government. To whom correspondence should be addressed: Dept. de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Casilla 114-D, Santiago, Chile. Fax: 56-2-635-5395; E-mail: ebrandan@genes.bio.puc.cl.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
N. Hempel, T. How, S. J. Cooper, T. R. Green, M. Dong, J. A. Copland, C. G. Wood, and G. C. Blobe
Expression of the type III TGF-{beta} receptor is negatively regulated by TGF-{beta}
Carcinogenesis, May 1, 2008; 29(5): 905 - 912.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. L. McCarthy, T. H. Pham, B. I. Knoll, and M. Centrella
Prostaglandin E2 Increases Transforming Growth Factor-{beta} Type III Receptor Expression through CCAAT Enhancer-Binding Protein {delta} in Osteoblasts
Mol. Endocrinol., November 1, 2007; 21(11): 2713 - 2724.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. G. Farnworth, Y. Wang, R. Escalona, P. Leembruggen, G. T. Ooi, and J. K. Findlay
Transforming Growth Factor-{beta} Blocks Inhibin Binding to Different Target Cell Types in a Context-Dependent Manner through Dual Mechanisms Involving Betaglycan
Endocrinology, November 1, 2007; 148(11): 5355 - 5368.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Cabello-Verrugio and E. Brandan
A Novel Modulatory Mechanism of Transforming Growth Factor-beta Signaling through Decorin and LRP-1
J. Biol. Chem., June 29, 2007; 282(26): 18842 - 18850.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Q. Zhu, A. R. Krakowski, E. E. Dunham, L. Wang, A. Bandyopadhyay, R. Berdeaux, G. S. Martin, L. Sun, and K. Luo
Dual Role of SnoN in Mammalian Tumorigenesis
Mol. Cell. Biol., January 1, 2007; 27(1): 324 - 339.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Brandan, C. Retamal, C. Cabello-Verrugio, and M.-P. Marzolo
The Low Density Lipoprotein Receptor-related Protein Functions as an Endocytic Receptor for Decorin
J. Biol. Chem., October 20, 2006; 281(42): 31562 - 31571.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Ugarte and E. Brandan
Transforming Growth Factor beta (TGF-beta) Signaling Is Regulated by Electrical Activity in Skeletal Muscle Cells: TGF-beta TYPE I RECEPTOR IS TRANSCRIPTIONALLY REGULATED BY MYOTUBE EXCITABILITY
J. Biol. Chem., July 7, 2006; 281(27): 18473 - 18481.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. B. de Leon, C. Montanez, P. Gomez, S. L. Morales-Lazaro, V. Tapia-Ramirez, V. Valadez-Graham, F. Recillas-Targa, D. Yaffe, U. Nudel, and B. Cisneros
Dystrophin Dp71 Expression Is Down-regulated during Myogenesis: ROLE OF Sp1 AND Sp3 ON THE Dp71 PROMOTER ACTIVITY
J. Biol. Chem., February 18, 2005; 280(7): 5290 - 5299.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. A. Sweeney and P. A. Johnson
Messenger RNA and Protein Expression Analysis of Betaglycan in the Pituitary and Ovary of the Domestic Hen
Biol Reprod, January 1, 2005; 72(1): 172 - 178.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
I. Barbosa, S. Garcia, V. Barbier-Chassefiere, J.-P. Caruelle, I. Martelly, and D. Papy-Garcia
Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies
Glycobiology, September 1, 2003; 13(9): 647 - 653.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement