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J. Biol. Chem., Vol. 278, Issue 1, 479-485, January 3, 2003
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From the Departments of Fibroblast growth factor 2 (FGF-2) is an
important modulator of cell growth and differentiation and a
neurotrophic factor. FGF-2 occurs in isoforms, at a low molecular
weight of 18,000 and at least two high molecular weight forms
(21,000 and 23,000), representing alternative translation products from
a single mRNA. In addition to its role as an extracellular ligand,
FGF-2 localizes to the nuclei of cells. Here we show differential
localization of the 18- and 23-kDa isoforms in the nuclei of rat
Schwann cells. Whereas the 18-kDa isoform was found in the nucleoli,
nucleoplasm, and Cajal bodies, the 23-kDa isoform localized in a
punctuate pattern and associates with mitotic chromosomes suggesting
different functional roles of the isoforms. Moreover, we show here that the 23-kDa FGF-2 isoform co-immunoprecipitates specifically with the
survival of motor neuron protein (SMN). SMN is an assembly and
recycling factor of the splicing machinery and locates to the
cytoplasm, the nucleoplasm, and nuclear gems, where it co-localizes with 23-kDa FGF-2. Patients with spinal muscular atrophy suffer from fatal degeneration of motoneurons because of mutations and deletions of the gene for the SMN protein.
Differential Intranuclear Localization of Fibroblast Growth
Factor-2 Isoforms and Specific Interaction with the Survival of
Motoneuron Protein*
§,,,,,
Neuroanatomy and
¶ Molecular and Cell Physiology, Hannover Medical School,
30625 Hannover, Germany
*
This work was supported by a HiLF grant from the Hannover
Medical School and a research grant from the Deutsche Gesellschaft für Muskelkranke (DGM e.V.; German Foundation for Muscular
Diseases) (to P. C.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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