| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 1, 545-555, January 3, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,
From the INSERM U 36 Collège de France Paris,
75005 Paris, France
Endothelin-converting enzyme (ECE) is a membrane
metalloprotease that generates endothelin from its direct precursor big
endothelin. Four isoforms of ECE-1 are produced from a single
gene through the use of alternate promoters. These isoforms share the
same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting. We
investigated the distribution of ECE-1 isoforms in transfected AtT-20
neuroendocrine cells. Whereas ECE-1a and 1c were present at the plasma
membrane, ECE-1b and ECE-1d were retained inside the cells. We found
that both intracellular isoforms were concentrated in the endosomal system: ECE-1d in recycling endosomes, and ECE-1b in late
endosomes/multivesicular bodies. Leucine-based motifs were involved in
the intracellular retention of these isoforms, and the targeting of
ECE-1b to the degradation pathway required an additional signal in the
N terminus. The concentration of ECE-1 isoforms in the endosomal system
suggested new functions for these enzymes. Potential novel functions
include redistribution of other isoforms through direct interaction. We have showed that ECE-1 isoforms could heterodimerize, and that in such
heterodimers the ECE-1b targeting signal was dominant. Interaction of a
plasma membrane isoform with ECE-1b resulted in its intracellular
localization and decreased its extracellular activity. These
data demonstrated that the targeting signals specific for ECE-1b
constitute a regulatory domain per se that could modulate the localization and the activity of other isoforms.
To whom correspondence should be addressed: INSERM U36,
Collège de France, 11 Place M.Berthelot, 75005 Paris, France.
Tel: 33-1-44-27-14-29; Fax: 33-1-44-27-16-91; E-mail:
laurent.muller@college-de-france.fr.
§
Present address: Dept. of Animal Science, Faculty of Agricultural,
Food and Environmental Quality Sciences, The Hebrew University of
Jerusalem, Rehovot 76100, Israel.
¶
Present address: AXOVAN AG, Gewerbestrasse 16, 4123 Allschwil, Switzerland.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  B. E. Padilla, G. S. Cottrell, D. Roosterman, S. Pikios, L. Muller, M. Steinhoff, and N. W. Bunnett Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and -arrestins J. Cell Biol., December 3, 2007; 179(5): 981 - 997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Roosterman, G. S. Cottrell, B. E. Padilla, L. Muller, C. B. Eckman, N. W. Bunnett, and M. Steinhoff Endothelin-converting enzyme 1 degrades neuropeptides in endosomes to control receptor recycling PNAS, July 10, 2007; 104(28): 11838 - 11843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Schefe, M. Menk, J. Reinemund, K. Effertz, R. M. Hobbs, P. P. Pandolfi, P. Ruiz, T. Unger, and H. Funke-Kaiser A Novel Signal Transduction Cascade Involving Direct Physical Interaction of the Renin/Prorenin Receptor With the Transcription Factor Promyelocytic Zinc Finger Protein Circ. Res., December 8, 2006; 99(12): 1355 - 1366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Roosterman, T. Goerge, S. W. Schneider, N. W. Bunnett, and M. Steinhoff Neuronal control of skin function: the skin as a neuroimmunoendocrine organ. Physiol Rev, October 1, 2006; 86(4): 1309 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Hunter and A. J. Turner Expression and localization of endothelin-converting enzyme-1 isoforms in human endothelial cells. Experimental Biology and Medicine, June 1, 2006; 231(6): 718 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Klipper, N. Levy, T. Gilboa, L. Muller, and R. Meidan Identification of a novel alternatively spliced variant endothelin converting enzyme-1 lacking a transmembrane domain. Experimental Biology and Medicine, June 1, 2006; 231(6): 723 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Dawson, N. J. Maitland, P. Berry, A. J. Turner, and B. A. Usmani Expression and localization of endothelin-converting enzyme-1 in human prostate cancer. Experimental Biology and Medicine, June 1, 2006; 231(6): 1106 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Meidan, E. Klipper, T. Gilboa, L. Muller, and N. Levy Endothelin-converting Enzyme-1, Abundance of Isoforms a-d and Identification of a Novel Alternatively Spliced Variant Lacking a Transmembrane Domain J. Biol. Chem., December 9, 2005; 280(49): 40867 - 40874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Wruck, H. Funke-Kaiser, T. Pufe, H. Kusserow, M. Menk, J. H. Schefe, M. L. Kruse, M. Stoll, and T. Unger Regulation of Transport of the Angiotensin AT2 Receptor by a Novel Membrane-Associated Golgi Protein Arterioscler. Thromb. Vasc. Biol., January 1, 2005; 25(1): 57 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Klipper, T. Gilboa, N. Levy, T. Kisliouk, K. Spanel-Borowski, and R. Meidan Characterization of endothelin-1 and nitric oxide generating systems in corpus luteum-derived endothelial cells Reproduction, October 1, 2004; 128(4): 463 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, D. Ma, J. Keski-Oja, and D. Pei Co-recycling of MT1-MMP and MT3-MMP through the Trans-Golgi Network: IDENTIFICATION OF DKV582 AS A RECYCLING SIGNAL J. Biol. Chem., March 5, 2004; 279(10): 9331 - 9336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tsuchisaka and A. Theologis Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene family PNAS, February 24, 2004; 101(8): 2275 - 2280. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
