JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M207223200 on November 27, 2002

J. Biol. Chem., Vol. 278, Issue 10, 7902-7909, March 7, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/10/7902    most recent
M207223200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klarmann, G. J.
Right arrow Articles by Preston, B. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klarmann, G. J.
Right arrow Articles by Preston, B. D.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Incorporation of Uracil into Minus Strand DNA Affects the Specificity of Plus Strand Synthesis Initiation during Lentiviral Reverse Transcription*

George J. KlarmannDagger §, Xin ChenDagger §, Thomas W. North||, and Bradley D. PrestonDagger **

From the Dagger  Eccles Institute of Human Genetics and the Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112 and the || Center for Comparative Medicine, University of California, Davis, California 95616

Many retroviruses either encode dUTP pyrophosphatase (dUTPase) or package host-derived uracil DNA glycosylase as a means to limit the accumulation of uracil in DNA strands, suggesting that uracil is detrimental to one or more steps in the viral life cycle. In the present study, the effects of DNA uracilation on (-) strand DNA synthesis, RNase H activity, and (+) strand DNA synthesis were investigated in a cell-free system. This system uses the activities of purified human immunodeficiency virus type 1 (HIV-1) reverse transcriptase to convert single-stranded RNA to double-stranded DNA in a single reaction mixture. Substitution of dUTP for dTTP had no effect on (-) strand synthesis but significantly decreased yields of (+) strand DNA. Mapping of nascent (+) strand 5' ends revealed that this was due to decreased initiation from polypurine tracts with a concomitant increase in initiation at non-polypurine tract sites. Aberrant initiation correlated with a change in RNase H cleavage specificity when assayed on preformed RNA-DNA duplexes containing uracilated DNA, suggesting that appropriate "selection" of the (+) strand primer is affected. Collectively, these data suggest that accumulation of uracil in retroviral DNA may disrupt the viral life cycle by altering the specificity of (+) strand DNA synthesis initiation during reverse transcription.


* This work was supported by United States Public Health Service Grants R01 AI34834, R01 AI38755, and P30 CA42014 (to B. D. P.) and R01 AI28189 (to T. W. N.) from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

To whom correspondence should be addressed: HIV Drug Resistance Program National Cancer Institute-Frederick, P. O. Box B, Frederick, MD 21702. Tel.: 301-846-5395; Fax: 301-846-6013; E-mail: gklarmann@ncifcrf.gov.

** Present address: University of Washington Dept. of Pathology K-072 Health Sciences Bldg., Box 357705, 1959 NE Pacific St., Seattle, WA 98195-7705.


Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Virol.Home page
J. L. Mbisa, R. Barr, J. A. Thomas, N. Vandegraaff, I. J. Dorweiler, E. S. Svarovskaia, W. L. Brown, L. M. Mansky, R. J. Gorelick, R. S. Harris, et al.
Human Immunodeficiency Virus Type 1 cDNAs Produced in the Presence of APOBEC3G Exhibit Defects in Plus-Strand DNA Transfer and Integration
J. Virol., July 1, 2007; 81(13): 7099 - 7110.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
R. Suspene, C. Rusniok, J.-P. Vartanian, and S. Wain-Hobson
Twin gradients in APOBEC3 edited HIV-1 DNA reflect the dynamics of lentiviral replication
Nucleic Acids Res., October 18, 2006; 34(17): 4677 - 4684.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
Y. Iwatani, H. Takeuchi, K. Strebel, and J. G. Levin
Biochemical Activities of Highly Purified, Catalytically Active Human APOBEC3G: Correlation with Antiviral Effect.
J. Virol., June 1, 2006; 80(12): 5992 - 6002.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
Y. Zhang, H. Moriyama, K. Homma, and J. L. Van Etten
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima
J. Virol., August 1, 2005; 79(15): 9945 - 9953.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. J. Wichroski, K. Ichiyama, and T. M. Rana
Analysis of HIV-1 Viral Infectivity Factor-mediated Proteasome-dependent Depletion of APOBEC3G: CORRELATING FUNCTION AND SUBCELLULAR LOCALIZATION
J. Biol. Chem., March 4, 2005; 280(9): 8387 - 8396.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. Chen, E. Le Rouzic, J. A. Kearney, L. M. Mansky, and S. Benichou
Vpr-mediated Incorporation of UNG2 into HIV-1 Particles Is Required to Modulate the Virus Mutation Rate and for Replication in Macrophages
J. Biol. Chem., July 2, 2004; 279(27): 28419 - 28425.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.