| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 10, 8163-8171, March 7, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Research Center for Glycoscience, National Institute of
Advanced Industrial Science and Technology, AIST Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
When transcription is initiated under repressive
conditions, such as when chromatin are packed together, binding
followed by the functioning of key components in the transcriptional
apparatus should be appropriately facilitated in the chromatin
architecture. We provide evidence that the erythroid-specific enhancer-
binding protein NF-E2 interacts with the cognate motif at DNase
I-hypersensitive site 2 of the human
Interaction of NF-E2 in the Human
-Globin Locus Control Region
before Chromatin Remodeling*
and
-globin locus control region in
a repressive state. The nucleosome containing the NF-E2-binding
site showed characteristic rotational and translational phases in
vitro. The binding site had less affinity to the histone octamers
than nearby regions while showing greater accessibility to DNase I and
micrococcal nuclease. Furthermore, the motif was recognized by the
exogenous NF-E2 protein expressed in HeLa cells, which have a
repressive state of chromatin at the -globin locus, as shown by
ligation-mediated PCR and chromatin immunoprecipitation assay. These
lines of evidence indicate that NF-E2 interacts with the cognate motif
on the nucleosome before chromatin is remodeled.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 81-298-61-6189;
Fax: 81-298-61-6190; E-mail: y-onishi@aist.go.jp.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Onishi, S. Hanai, T. Ohno, Y. Hara, and N. Ishida Rhythmic SAF-A Binding Underlies Circadian Transcription of the Bmal1 Gene Mol. Cell. Biol., May 15, 2008; 28(10): 3477 - 3488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Yamada, K. Ozaki, M. Nakatake, M. Akiyama, K. Kawauchi, and R. Matsuoka Multistep regulation of telomerase during differentiation of HL60 cells J. Leukoc. Biol., May 1, 2008; 83(5): 1240 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ohno, Y. Onishi, and N. Ishida A novel E4BP4 element drives circadian expression of mPeriod2 Nucleic Acids Res., January 28, 2007; 35(2): 648 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Vieira, P. P. Levings, M. A. Hill, V. J. Crusselle, S.-H. L. Kang, J. D. Engel, and J. Bungert Recruitment of Transcription Complexes to the {beta}-Globin Gene Locus in Vivo and in Vitro J. Biol. Chem., November 26, 2004; 279(48): 50350 - 50357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wada-Kiyama, M. Kato, Y. Onishi, and R. Kiyama A Conserved Regulatory Element in the Mammalian {beta}-Globin Promoters. Blood (ASH Annual Meeting Abstracts), November 16, 2004; 104(11): 1217 - 1217. [Abstract]
|
|
|
|
|
|
G. Kronke, V. N. Bochkov, J. Huber, F. Gruber, S. Bluml, A. Furnkranz, A. Kadl, B. R. Binder, and N. Leitinger Oxidized Phospholipids Induce Expression of Human Heme Oxygenase-1 Involving Activation of cAMP-responsive Element-binding Protein J. Biol. Chem., December 19, 2003; 278(51): 51006 - 51014. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
