JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M211211200 on December 31, 2002

J. Biol. Chem., Vol. 278, Issue 10, 8653-8660, March 7, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/10/8653    most recent
M211211200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nurse, P.
Right arrow Articles by Marians, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nurse, P.
Right arrow Articles by Marians, K. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Topoisomerase III Can Serve as the Cellular Decatenase in Escherichia coli*

Pearl Nurse, Cindy Levine, Heide Hassing, and Kenneth J. MariansDagger

From the Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021

topB, encoding topoisomerase III, was identified as a high copy suppressor of the temperature-sensitive parC1215 allele, encoding one of the subunits of topoisomerase IV. Overexpression of topoisomerase III at the nonpermissive temperature was shown subsequently to restore timely chromosome decatenation and suppress lethality in strains carrying either temperature-sensitive parE or parC alleles. By developing an assay in vitro for precatenane unlinking, we demonstrated directly that both topoisomerase III and topoisomerase IV were efficient at this task, whereas DNA gyrase was very inefficient at precatenane removal. These observations suggest that precatenane unlinking is sufficient to sustain decatenation of replicating daughter chromosomes in the cell.

* This work was supported by National Institutes of Health Grant GM34558.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Ave., New York, NY 10021. Tel.: 212-639-5890; Fax: 212-717-3627; E-mail: k-marians@ski.mskcc.org.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
S. D. Brown, M. R. Thompson, N. C. VerBerkmoes, K. Chourey, M. Shah, J. Zhou, R. L. Hettich, and D. K. Thompson
Molecular Dynamics of the Shewanella oneidensis Response to Chromate Stress
Mol. Cell. Proteomics, June 1, 2006; 5(6): 1054 - 1071.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. L. Chen, C.-S. Hung, J. Xu, C. S. Reigstad, V. Magrini, A. Sabo, D. Blasiar, T. Bieri, R. R. Meyer, P. Ozersky, et al.
Identification of genes subject to positive selection in uropathogenic strains of Escherichia coli: A comparative genomics approach
PNAS, April 11, 2006; 103(15): 5977 - 5982.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Yamashiro and A. Yamagishi
Characterization of the DNA Gyrase from the Thermoacidophilic Archaeon Thermoplasma acidophilum
J. Bacteriol., December 15, 2005; 187(24): 8531 - 8536.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. R. Mullen, F. S. Nallaseth, Y. Q. Lan, C. E. Slagle, and S. J. Brill
Yeast Rmi1/Nce4 Controls Genome Stability as a Subunit of the Sgs1-Top3 Complex
Mol. Cell. Biol., June 1, 2005; 25(11): 4476 - 4487.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Plank, S. H. Chu, J. R. Pohlhaus, T. Wilson-Sali, and T.-s. Hsieh
Drosophila melanogaster Topoisomerase III{alpha} Preferentially Relaxes a Positively or Negatively Supercoiled Bubble Substrate and Is Essential during Development
J. Biol. Chem., February 4, 2005; 280(5): 3564 - 3573.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Ince and D. C. Hooper
Quinolone Resistance Due to Reduced Target Enzyme Expression
J. Bacteriol., December 1, 2003; 185(23): 6883 - 6892.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. Espeli, C. Lee, and K. J. Marians
A Physical and Functional Interaction between Escherichia coli FtsK and Topoisomerase IV
J. Biol. Chem., November 7, 2003; 278(45): 44639 - 44644.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. G. Harmon, J. P. Brockman, and S. C. Kowalczykowski
RecQ Helicase Stimulates Both DNA Catenation and Changes in DNA Topology by Topoisomerase III
J. Biol. Chem., October 24, 2003; 278(43): 42668 - 42678.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Charvin, D. Bensimon, and V. Croquette
Single-molecule study of DNA unlinking by eukaryotic and prokaryotic type-II topoisomerases
PNAS, August 19, 2003; 100(17): 9820 - 9825.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. J. Sherratt
Bacterial Chromosome Dynamics
Science, August 8, 2003; 301(5634): 780 - 785.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.