Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M207542200 on December 23, 2002

J. Biol. Chem., Vol. 278, Issue 11, 9052-9057, March 14, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/11/9052    most recent
M207542200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Vliet, A. H. M.
Right arrow Articles by Kusters, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Vliet, A. H. M.
Right arrow Articles by Kusters, J. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Differential Regulation of Amidase- and Formamidase-mediated Ammonia Production by the Helicobacter pylori Fur Repressor*

Arnoud H. M. van VlietDagger §, Jeroen StoofDagger , Sophie W. PoppelaarsDagger , Stefan Bereswill, Georg Homuth||, Manfred Kist, Ernst J. KuipersDagger , and Johannes G. KustersDagger

From the Dagger  Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands, the  Department of Medical Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University Hospital of Freiburg, D-79104 Freiburg, Germany, and the || Institute for Microbiology, University of Greifswald, D-17487 Greifswald, Germany

The production of high levels of ammonia allows the human gastric pathogen Helicobacter pylori to survive the acidic conditions in the human stomach. H. pylori produces ammonia through urease-mediated degradation of urea, but it is also able to convert a range of amide substrates into ammonia via its AmiE amidase and AmiF formamidase enzymes. Here data are provided that demonstrate that the iron-responsive regulatory protein Fur directly and indirectly regulates the activity of the two H. pylori amidases. In contrast to other amidase-positive bacteria, amidase and formamidase enzyme activities were not induced by medium supplementation with their respective substrates, acrylamide and formamide. AmiE protein expression and amidase enzyme activity were iron-repressed in H. pylori 26695 but constitutive in the isogenic fur mutant. This regulation was mediated at the transcriptional level via the binding of Fur to the amiE promoter region. In contrast, formamidase enzyme activity was not iron-repressed but was significantly higher in the fur mutant. This effect was not mediated at the transcriptional level, and Fur did not bind to the amiF promoter region. These roles of Fur in regulation of the H. pylori amidases suggest that the H. pylori Fur regulator may have acquired extra functions to compensate for the absence of other regulatory systems.

* This work was supported by in part by Grants 901-14-206 and DN93-340 from the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (to A. H. M. v. V. and J. G. K.), respectively, and Grant Ki201/9-1 of the Deutsche Forschungsgemeinschaft (to M. K.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Gastroenterology and Hepatology, Rm. L-455, Erasmus MC-University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Tel.: 31-10-4635944; Fax: 31-10-4632793; E-mail: a.h.m.vanvliet{at}erasmusmc.nl.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
B. M. Carpenter, J. M. Whitmire, and D. S. Merrell
This Is Not Your Mother's Repressor: the Complex Role of Fur in Pathogenesis
Infect. Immun., July 1, 2009; 77(7): 2590 - 2601.
[Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
B. M. Carpenter, T. K. McDaniel, J. M. Whitmire, H. Gancz, S. Guidotti, S. Censini, and D. S. Merrell
Expanding the Helicobacter pylori Genetic Toolbox: Modification of an Endogenous Plasmid for Use as a Transcriptional Reporter and Complementation Vector
Appl. Envir. Microbiol., December 1, 2007; 73(23): 7506 - 7514.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
C. Belzer, B. A. M. van Schendel, E. J. Kuipers, J. G. Kusters, and A. H. M. van Vliet
Iron-Responsive Repression of Urease Expression in Helicobacter hepaticus Is Mediated by the Transcriptional Regulator Fur
Infect. Immun., February 1, 2007; 75(2): 745 - 752.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
J. G. Kusters, A. H. M. van Vliet, and E. J. Kuipers
Pathogenesis of Helicobacter pylori Infection
Clin. Microbiol. Rev., July 1, 2006; 19(3): 449 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. T. Loh and T. L. Cover
Requirement of Histidine Kinases HP0165 and HP1364 for Acid Resistance in Helicobacter pylori.
Infect. Immun., May 1, 2006; 74(5): 3052 - 3059.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Pflock, N. Finsterer, B. Joseph, H. Mollenkopf, T. F. Meyer, and D. Beier
Characterization of the ArsRS Regulon of Helicobacter pylori, Involved in Acid Adaptation.
J. Bacteriol., May 1, 2006; 188(10): 3449 - 3462.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
H. Gancz, S. Censini, and D. S. Merrell
Iron and pH Homeostasis Intersect at the Level of Fur Regulation in the Gastric Pathogen Helicobacter pylori
Infect. Immun., January 1, 2006; 74(1): 602 - 614.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
I. Delany, R. Ieva, A. Soragni, M. Hilleringmann, R. Rappuoli, and V. Scarlato
In Vitro Analysis of Protein-Operator Interactions of the NikR and Fur Metal-Responsive Regulators of Coregulated Genes in Helicobacter pylori
J. Bacteriol., November 15, 2005; 187(22): 7703 - 7715.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
F. D. Ernst, E. J. Kuipers, A. Heijens, R. Sarwari, J. Stoof, C. W. Penn, J. G. Kusters, and A. H. M. van Vliet
The Nickel-Responsive Regulator NikR Controls Activation and Repression of Gene Transcription in Helicobacter pylori
Infect. Immun., November 1, 2005; 73(11): 7252 - 7258.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
M. Pflock, S. Kennard, I. Delany, V. Scarlato, and D. Beier
Acid-Induced Activation of the Urease Promoters Is Mediated Directly by the ArsRS Two-Component System of Helicobacter pylori
Infect. Immun., October 1, 2005; 73(10): 6437 - 6445.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
F. D. Ernst, G. Homuth, J. Stoof, U. Mader, B. Waidner, E. J. Kuipers, M. Kist, J. G. Kusters, S. Bereswill, and A. H. M. van Vliet
Iron-Responsive Regulation of the Helicobacter pylori Iron-Cofactored Superoxide Dismutase SodB Is Mediated by Fur
J. Bacteriol., June 1, 2005; 187(11): 3687 - 3692.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
F. D. Ernst, S. Bereswill, B. Waidner, J. Stoof, U. Mader, J. G. Kusters, E. J. Kuipers, M. Kist, A. H. M. van Vliet, and G. Homuth
Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expression
Microbiology, February 1, 2005; 151(2): 533 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
A. H. M. van Vliet, E. J. Kuipers, J. Stoof, S. W. Poppelaars, and J. G. Kusters
Acid-Responsive Gene Induction of Ammonia-Producing Enzymes in Helicobacter pylori Is Mediated via a Metal-Responsive Repressor Cascade
Infect. Immun., February 1, 2004; 72(2): 766 - 773.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement