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J. Biol. Chem., Vol. 278, Issue 11, 9185-9194, March 14, 2003
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From the Institute of Microbial Technology, Sector 39A,
Chandigarh-160 036, India and the Mating-type silencing in
Schizosaccharomyces pombe is brought about by cooperative
interactions between cis-acting DNA sequences flanking
mat2P and mat3M and the trans-acting factors,
namely Swi6, Clr1-Clr4, Clr6, and Rik1. In addition, DNA repair gene rhp6, which plays a role in post-replication DNA repair and
ubiquitination of proteins including histones, is also involved in
silencing, albeit in a unique way; its effect on silencing and
chromatin structure of the donor loci is dependent on their switching
competence. Earlier, we hypothesized the existence of a mediator of
Rhp6 that plays a role in reestablishment of the chromatin structure
coincidentally with DNA replication associated with mating-type
switching. Here we report the identification of a 22-kDa protein as an
in vivo target and mediator of Rhp6 in mating-type
silencing. The level of this protein is greatly elevated in
sng1-1/rhp6
Identification of Uhp1, a Ubiquitinated Histone-like Protein, as
a Target/Mediator of Rhp6 in Mating-type Silencing in Fission
Yeast*
§, Department of
Pathology, Stanford University, Stanford, Californina 94305-5324
mutant and
rhp6
as compared with wild type strain. Both the deletion and overexpression of the gene encoding this protein elicit
switching-dependent loss of silencing. Furthermore, the 22-kDa protein undergoes Rhp6-dependent multiubiquitination
and associates with mat2 locus during S phase in wild type
cells. Interestingly, it contains a histone-fold motif similar to that of histone H2A, and like histone H2A, it interacts strongly with histone H2B in vitro. These results indicate that the
22-kDa protein, renamed as the ubiquitinated histone-like protein Uhp1,
is an in vivo target/mediator of Rhp6 in silencing. Thus,
regulation of association of Uhp1 with chromatin and ubiquitination
followed by degradation may play a role in reestablishment of inactive chromatin structure at the silent mating-type loci.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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