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J. Biol. Chem., Vol. 278, Issue 11, 9318-9321, March 14, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/11/9318    most recent M208347200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pang, T.-L. Articles by Lin, J.-J. Search for Related Content PubMed PubMed Citation Articles by Pang, T.-L. Articles by Lin, J.-J. Social Bookmarking                      What's this?

Exposure of Single-stranded Telomeric DNA Causes G₂/M Cell Cycle Arrest in Saccharomyces cerevisiae^*

Te-Ling Pang^§¶, Chen-Yi Wang^§¶, Chia-Ling Hsu, Mei-Yu Chen^§, and Jing-Jer Lin

From the Institutes of  Biopharmaceutical Science and ^§ Biochemistry, National Yang-Ming University, Taipei 112, Taiwan, Republic of China

In Saccharomyces cerevisiae, Cdc13p is a single-stranded TG_1-3 DNA binding protein that protects telomeres and maintains telomere length. A mutant allele of CDC13, cdc13-1, causes accumulation of single-stranded TG_1-3 DNA near telomeres along with a G₂/M cell cycle arrest at non-permissive temperatures. We report here that when the single-stranded TG_1-3 DNA is masked by its binding proteins, such as S. cerevisiae Gbp2p or Schizosaccharomyces pombe Tcg1, the growth arrest phenotype of cdc13-1 is rescued. Mutations on Gbp2p that disrupt its binding to the single-stranded TG_1-3 DNA render the protein unable to complement the defects of cdc13-1. These results indicate that the presence of a single-stranded TG_1-3 tail in cdc13-1 cells serves as the signal for the cell cycle checkpoint. Moreover, the binding activity of Gbp2p to single-stranded TG_1-3 DNA appears to be associated with its ability to restore the telomere-lengthening phenotype in cdc13-1 cells. These results indicate that Gbp2p is involved in modulating telomere length.

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