JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M208937200 on December 31, 2002

J. Biol. Chem., Vol. 278, Issue 11, 9576-9584, March 14, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/11/9576    most recent
M208937200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thodeti, C. K.
Right arrow Articles by Wewer, U. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thodeti, C. K.
Right arrow Articles by Wewer, U. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ADAM12/Syndecan-4 Signaling Promotes beta 1 Integrin-dependent Cell Spreading through Protein Kinase Calpha and RhoA*

Charles Kumar ThodetiDagger , Reidar AlbrechtsenDagger , Morten GrauslundDagger , Meena AsmarDagger , Christer Larsson§, Yoshikazu Takada, Arthur M. Mercurio||, John R. Couchman**, and Ulla M. WewerDagger Dagger Dagger

From the Dagger  Institute of Molecular Pathology, University of Copenhagen, Frederik V's vej 11, DK-2100, Copenhagen, Denmark, the § Division of Molecular Medicine, Lund University, SE-20502 Malmö, Sweden, the  Department of Vascular Biology VB-1, The Scripps Research Institute, La Jolla, California 92037, the || Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, and the ** Division of Biomedical Sciences, Imperial College London, SW7 2AZ, London, United Kingdom

The ADAMs (a disintegrin and metalloprotease) comprise a large family of multidomain proteins with cell-binding and metalloprotease activities. The ADAM12 cysteine-rich domain (rADAM12-cys) supports cell attachment using syndecan-4 as a primary cell surface receptor that subsequently triggers beta 1 integrin-dependent cell spreading, stress fiber assembly, and focal adhesion formation. This process contrasts with cell adhesion on fibronectin, which is integrin-initiated but syndecan-4-dependent. In the present study, we investigated ADAM12/syndecan-4 signaling leading to cell spreading and stress fiber formation. We demonstrate that syndecan-4, when present in significant amounts, promotes beta 1 integrin-dependent cell spreading and stress fiber formation in response to rADAM12-cys. A mutant form of syndecan-4 deficient in protein kinase C (PKC)alpha activation or a different member of the syndecan family, syndecan-2, was unable to promote cell spreading. GF109203X and Gö6976, inhibitors of PKC, completely inhibited ADAM12/syndecan-4-induced cell spreading. Expression of syndecan-4, but not syn4Delta I, resulted in the accumulation of activated beta 1 integrins at the cell periphery in Chinese hamster ovary beta 1 cells as revealed by 12G10 staining. Further, expression of myristoylated, constitutively active PKCalpha resulted in beta 1 integrin-dependent cell spreading, but additional activation of RhoA was required to induce stress fiber formation. In summary, these data provide novel insights into syndecan-4 signaling. Syndecan-4 can promote cell spreading in a beta 1 integrin-dependent fashion through PKCalpha and RhoA, and PKCalpha and RhoA likely function in separate pathways.

* This work was supported by grants from the Danish Cancer Society, The Danish Medical Research Council, The Neye-Foundation, Novo Nordisk, Haensch, Munksholm, Velux, and Dansk Kraeftforsknings Fond (to U. W.), Wellcome Trust Program Grant 065940, and National Institutes of Health Grants GM50194 (to J. R. C.) and CA80789 (to A. M. M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Dagger To whom correspondence should be addressed: Institute of Molecular Pathology, University of Copenhagen, Frederik V's vej 11, DK-2100, Copenhagen, Denmark. Tel.: 45-3532-6056; Fax: 45-3532-6081; E-mail: ullaw@pai.ku.dk.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Saito, H. Imazeki, S. Miura, T. Yoshimura, H. Okutsu, Y. Harada, T. Ohwaki, O. Nagao, S. Kamiya, R. Hayashi, et al.
A Peptide Derived from Tenascin-C Induces 1 Integrin Activation through Syndecan-4
J. Biol. Chem., November 30, 2007; 282(48): 34929 - 34937.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Zigrino, J. Steiger, J. W. Fox, S. Loffek, A. Schild, R. Nischt, and C. Mauch
Role of ADAM-9 Disintegrin-Cysteine-rich Domains in Human Keratinocyte Migration
J. Biol. Chem., October 19, 2007; 282(42): 30785 - 30793.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Shimoda, G. Hashimoto, S. Mochizuki, E. Ikeda, N. Nagai, S. Ishida, and Y. Okada
Binding of ADAM28 to P-selectin Glycoprotein Ligand-1 Enhances P-selectin-mediated Leukocyte Adhesion to Endothelial Cells
J. Biol. Chem., August 31, 2007; 282(35): 25864 - 25874.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. Cheshenko, W. Liu, L. M. Satlin, and B. C. Herold
Multiple Receptor Interactions Trigger Release of Membrane and Intracellular Calcium Stores Critical for Herpes Simplex Virus Entry
Mol. Biol. Cell, August 1, 2007; 18(8): 3119 - 3130.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Dovas, A. Yoneda, and J. R. Couchman
PKC{alpha}-dependent activation of RhoA by syndecan-4 during focal adhesion formation
J. Cell Sci., July 1, 2006; 119(13): 2837 - 2846.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. J. McQuade, D. M. Beauvais, B. J. Burbach, and A. C. Rapraeger
Syndecan-1 regulates {alpha}v{beta}5 integrin activity in B82L fibroblasts
J. Cell Sci., June 15, 2006; 119(12): 2445 - 2456.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Tkachenko, A. Elfenbein, D. Tirziu, and M. Simons
Syndecan-4 Clustering Induces Cell Migration in a PDZ-Dependent Manner
Circ. Res., June 9, 2006; 98(11): 1398 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
S. Brule, N. Charnaux, A. Sutton, D. Ledoux, T. Chaigneau, L. Saffar, and L. Gattegno
The shedding of syndecan-4 and syndecan-1 from HeLa cells and human primary macrophages is accelerated by SDF-1/CXCL12 and mediated by the matrix metalloproteinase-9
Glycobiology, June 1, 2006; 16(6): 488 - 501.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
M. F. Smith Jr., J. Novotny, V. S. Carl, and L. D. Comeau
Helicobacter pylori and toll-like receptor agonists induce syndecan-4 expression in an NF-{kappa}B-dependent manner
Glycobiology, March 1, 2006; 16(3): 221 - 229.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
F. Feuerhake, J. L. Kutok, S. Monti, W. Chen, A. S. LaCasce, G. Cattoretti, P. Kurtin, G. S. Pinkus, L. de Leval, N. L. Harris, et al.
NF{kappa}B activity, function, and target-gene signatures in primary mediastinal large B-cell lymphoma and diffuse large B-cell lymphoma subtypes
Blood, August 15, 2005; 106(4): 1392 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. H. Rauch, E. Millette, R. D. Kenagy, G. Daum, J. W. Fischer, and A. W. Clowes
Syndecan-4 Is Required for Thrombin-induced Migration and Proliferation in Human Vascular Smooth Muscle Cells
J. Biol. Chem., April 29, 2005; 280(17): 17507 - 17511.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. Lafuste, C. Sonnet, B. Chazaud, P. A. Dreyfus, R. K. Gherardi, U. M. Wewer, and F.-J. Authier
ADAM12 and {alpha}9{beta}1 Integrin Are Instrumental in Human Myogenic Cell Differentiation
Mol. Biol. Cell, February 1, 2005; 16(2): 861 - 870.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Sundberg, C. K. Thodeti, M. Kveiborg, C. Larsson, P. Parker, R. Albrechtsen, and U. M. Wewer
Regulation of ADAM12 Cell-surface Expression by Protein Kinase C {epsilon}
J. Biol. Chem., December 3, 2004; 279(49): 51601 - 51611.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. V. Truong, M. M. Monick, T. O. Yarovinsky, L. S. Powers, T. Nyunoya, and G. W. Hunninghake
Extracellular Signal-Regulated Kinase Activation Delays Hyperoxia-Induced Epithelial Cell Death in Conditions of Akt Downregulation
Am. J. Respir. Cell Mol. Biol., December 1, 2004; 31(6): 611 - 618.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Zhao, K. E. Ramsey, D. A. Stephan, and P. Russell
Gene and Protein Expression Changes in Human Trabecular Meshwork Cells Treated with Transforming Growth Factor-{beta}
Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 4023 - 4034.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
K. Ishida, G. Hirai, K. Murakami, T. Teruya, S. Simizu, M. Sodeoka, and H. Osada
Structure-based design of a selective heparanase inhibitor as an antimetastatic agent
Mol. Cancer Ther., September 1, 2004; 3(9): 1069 - 1077.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. Moghadaszadeh, R. Albrechtsen, L. T. Guo, M. Zaik, N. Kawaguchi, R. H. Borup, P. Kronqvist, H. D. Schroder, K. E. Davies, T. Voit, et al.
Compensation for dystrophin-deficiency: ADAM12 overexpression in skeletal muscle results in increased {alpha}7 integrin, utrophin and associated glycoproteins
Hum. Mol. Genet., October 1, 2003; 12(19): 2467 - 2479.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Utani, Y. Momota, H. Endo, Y. Kasuya, K. Beck, N. Suzuki, M. Nomizu, and H. Shinkai
Laminin {alpha}3 LG4 Module Induces Matrix Metalloproteinase-1 through Mitogen-activated Protein Kinase Signaling
J. Biol. Chem., September 5, 2003; 278(36): 34483 - 34490.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.