HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 11, 9698-9705, March 14, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/11/9698    most recent M208452200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Paradis, E. Articles by Ven Murthy, M. R. Search for Related Content PubMed PubMed Citation Articles by Paradis, E. Articles by Ven Murthy, M. R. Social Bookmarking                      What's this?

Lipoprotein Lipase Affects the Survival and Differentiation of Neural Cells Exposed to Very Low Density Lipoprotein^*

Eric Paradis, Sébastien Clément, Pierre Julien^§, and M. R. Ven Murthy^¶

From the  Department of Medical Biology, Faculty of Medicine and ^§ Lipid Research Centre, Laval University Medical Research Centre, Laval University, Ste-Foy, Quebec G1K 7P4, Canada

Lipoprotein lipase (LPL) is a key enzyme involved in the metabolism of lipoproteins, providing tissues like adipose tissue or skeletal muscle with fatty acids. LPL is also expressed in the brain, fulfilling yet unknown functions. Using a neuroblastoma cell line transfected with a NEO- or a LPL-expression vector, we have developed a model to study the function of LPL in neurons exposed to native or copper-oxidized lipoproteins. The addition to the culture media of VLDL with 10 µM copper sulfate led to a significant reduction in the viability of NEO transfectants whereas LPL-transfectants were protected from this injury. In the presence of VLDL and CuSO₄, LPL transfectants were even able to display significant neurite extension. This neuritogenic effect was also observed in LPL transfectants exposed to native lipoproteins. However, addition of VLDL particles oxidized with CuSO₄ prior to their addition to the culture media resulted in neurotoxic effects on LPL transfectants. These findings suggest that the presence of LPL in cultured neuronal cells modulates the physiological response of neurons following exposure to native or oxidized lipoproteins. LPL could thus play a key role in the differentiation of Neuro-2A cells and in the pathophysiological effects of oxidative stress in several neurodegenerative disorders.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				X. Xian, T. Liu, J. Yu, Y. Wang, Y. Miao, J. Zhang, Y. Yu, C. Ross, J. M. Karasinska, M. R. Hayden, et al. Presynaptic Defects Underlying Impaired Learning and Memory Function in Lipoprotein Lipase-Deficient Mice J. Neurosci., April 8, 2009; 29(14): 4681 - 4685. [Abstract] [Full Text] [PDF]

				K. Abildayeva, J. F. P. Berbee, A. Blokland, P. J. Jansen, F. J. Hoek, O. Meijer, D. Lutjohann, T. Gautier, T. Pillot, J. De Vente, et al. Human apolipoprotein C-I expression in mice impairs learning and memory functions J. Lipid Res., April 1, 2008; 49(4): 856 - 869. [Abstract] [Full Text] [PDF]

				J. G. Greene Gene expression profiles of brain dopamine neurons and relevance to neuropsychiatric disease J. Physiol., September 1, 2006; 575(2): 411 - 416. [Abstract] [Full Text] [PDF]

				J. Grimm, A. Mueller, F. Hefti, and A. Rosenthal Molecular basis for catecholaminergic neuron diversity PNAS, September 21, 2004; 101(38): 13891 - 13896. [Abstract] [Full Text] [PDF]