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Originally published In Press as doi:10.1074/jbc.M208694200 on January 9, 2003

J. Biol. Chem., Vol. 278, Issue 12, 10041-10047, March 21, 2003
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Identification of a Novel Protein, PDIP38, That Interacts with the p50 Subunit of DNA Polymerase delta  and Proliferating Cell Nuclear Antigen*

Li Liu, Esther M. Rodriguez-Belmonte, Nayef Mazloum, Bin Xie, and Marietta Y. W. T. LeeDagger

From the Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595

The yeast two-hybrid screening method was used to identify novel proteins that associate with human DNA polymerase delta  (pol delta ). Two baits were used in this study. These were the large (p125) and small (p50) subunits of the core pol delta  heterodimer. p50 was the only positive isolated with p125 as the bait. Two novel protein partners, named PDIP38 and PDIP46, were identified from the p50 screen. In this study, the interaction of PDIP38 with pol delta  was further characterized. PDIP38 encodes a protein of 368 amino acids whose C terminus is conserved with the bacterial APAG protein and with the F box A protein. It was found that PDIP38 also interacts with proliferating cell nuclear antigen (PCNA). The ability of PDIP38 to interact with both the p50 subunit of pol delta  and with PCNA was confirmed by pull-down assays using glutathione S-transferase (GST)-PDIP38 fusion proteins. The PCNA-PDIP38 interaction was also demonstrated by PCNA overlay experiments. The association of PDIP38 with pol delta  was shown to occur in calf thymus tissue and mammalian cell extracts by GST-PDIP38 pull-down and coimmunoprecipitation experiments. PDIP38 was associated with pol delta  isolated by immunoaffinity chromatography. The association of PDIP38 with pol delta  could also be demonstrated by native gel electrophoresis.

* This work was supported by National Institutes of Health Grant GM31973.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Valhalla, NY 10595. Tel.: 914-594-4070; Fax: 914-594-4058; E-mail: Marietta_Lee@nymc.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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