HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 12, 10102-10111, March 21, 2003

This Article Full Text Full Text (PDF) An addition or correction has been published All Versions of this Article: 278/12/10102    most recent M210201200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Raja, A. Articles by DeStefano, J. J. Search for Related Content PubMed PubMed Citation Articles by Raja, A. Articles by DeStefano, J. J. Social Bookmarking                      What's this?

Interaction of HIV Reverse Transcriptase with Structures Mimicking Recombination Intermediates^*

Aarti Raja and Jeffrey J. DeStefano^§

From the Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, Maryland 20742

Interactions between human immunodeficiency virus (HIV) reverse transcriptase (RT) and structures mimicking intermediates proposed to occur during recombination (strand transfer) were investigated. One mechanism proposed for strand transfer is strand exchange in which a homologous RNA (acceptor) "invades" a donor RNA·DNA duplex (replication intermediate) on which DNA synthesis is occurring. The acceptor displaces the donor of the duplex and binds to the DNA. During exchange a transient trimeric structure forms. A model structure was designed with a replication intermediate to which an acceptor RNA was bound. The acceptor was bound to the 5'-end of the DNA over a 54-base region, whereas the donor associated with the DNA 3'-end over a 28-base region. The dimeric constituents of the trimer (acceptor RNA·DNA and donor RNA·DNA) were also constructed. The acceptor RNA·DNA formed a branched structure in this case. Results showed that RT could cleave the RNA portion of all the structures examined. Association with junction substrates was less stable as determined by off-rates. On the trimer, RT cleaved both RNAs but showed a clear preference for cleaving the donor RNA region. This preference was accentuated by HIV nucleocapsid protein (NC). Results suggest that during recombination RT generally associates with the donor-RNA portion of the trimer and the acceptor RNA is protected but not immune from cleavage. The partial protection likely allows the acceptor RNA to more easily complete strand exchange and shield this RNA to provide a means to salvage replication if the DNA were to dissociate from the cleaved donor RNA.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Chen, M. Balakrishnan, B. P. Roques, and R. A. Bambara Acceptor RNA Cleavage Profile Supports an Invasion Mechanism for HIV-1 Minus Strand Transfer J. Biol. Chem., April 15, 2005; 280(15): 14443 - 14452. [Abstract] [Full Text] [PDF]

				T. D. Rhodes, O. Nikolaitchik, J. Chen, D. Powell, and W.-S. Hu Genetic Recombination of Human Immunodeficiency Virus Type 1 in One Round of Viral Replication: Effects of Genetic Distance, Target Cells, Accessory Genes, and Lack of High Negative Interference in Crossover Events J. Virol., February 1, 2005; 79(3): 1666 - 1677. [Abstract] [Full Text] [PDF]

				R. Galetto, A. Moumen, V. Giacomoni, M. Veron, P. Charneau, and M. Negroni The Structure of HIV-1 Genomic RNA in the gp120 Gene Determines a Recombination Hot Spot in Vivo J. Biol. Chem., August 27, 2004; 279(35): 36625 - 36632. [Abstract] [Full Text] [PDF]