|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 12, 10291-10296, March 21, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Molecular Biology and Biotechnology,
Graduate school of Pharmaceutical Sciences, Chiba University, CREST of
Japan Science and Technology Corporation, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan
Cystine lyases catalyze the breakdown of
L-cystine to thiocysteine, pyruvate, and ammonia.
Until now there are no reports of the identification of a plant cystine
lyase at a molecular level, and it is not clear what biological role
this class of enzymes have in plants. A cystine lyase was isolated from
Brassica oleracea (L.), and partial amino acid sequencing
allowed the corresponding full-length cDNA (BOCL3) to
be cloned. The deduced amino acid sequence of BOCL3 showed
highest homology to the deduced amino acid sequences of several
Arabidopsis thaliana genes annotated as tyrosine
aminotransferase-like, including a coronatine, jasmonic acid, and salt
stress-inducible gene, CORI3 (78.8% identity), and the
unidentified rooty/superroot1 gene (44.8%
identity). A full-length expressed sequence tag clone of
CORI3 was obtained and recombinant CORI3 was synthesized in
Escherichia coli. Isolated recombinant CORI3 catalyzed a
cystine lyase reaction, but no aminotransferase reactions. The
present study identifies, for the first time, a cystine lyase from
plants at a molecular level and redefines the functional assignment of
the only functionally identified member of a group of A. thaliana genes annotated as tyrosine
aminotransferase-like.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY187682.
A New Member of Plant CS-lyases
A CYSTINE LYASE FROM ARABIDOPSIS THALIANA*
,
*
This work was supported in part by grants-in-aid for
Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan and by CREST of Japan Science and Technology.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a postdoctoral fellowship from the Japan Society for
the Promotion of Science.
§
To whom correspondence should be addressed. Tel.: 81-43-290-2904;
Fax: 81-43-290-2905; E-mail: ksaito@p.chiba-u.ac.jp.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. C. Rowe and D. J. Kliebenstein Complex Genetics Control Natural Variation in Arabidopsis thaliana Resistance to Botrytis cinerea Genetics, December 1, 2008; 180(4): 2237 - 2250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Obayashi, K. Kinoshita, K. Nakai, M. Shibaoka, S. Hayashi, M. Saeki, D. Shibata, K. Saito, and H. Ohta ATTED-II: a database of co-expressed genes and cis elements for identifying co-regulated gene groups in Arabidopsis Nucleic Acids Res., January 12, 2007; 35(suppl_1): D863 - D869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Campanini, F. Schiaretti, S. Abbruzzetti, D. Kessler, and A. Mozzarelli Sulfur Mobilization in Cyanobacteria: THE CATALYTIC MECHANISM OF L-CYSTINE C-S LYASE (C-DES) FROM SYNECHOCYSTIS J. Biol. Chem., December 15, 2006; 281(50): 38769 - 38780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Hirai, M. Klein, Y. Fujikawa, M. Yano, D. B. Goodenowe, Y. Yamazaki, S. Kanaya, Y. Nakamura, M. Kitayama, H. Suzuki, et al. Elucidation of Gene-to-Gene and Metabolite-to-Gene Networks in Arabidopsis by Integration of Metabolomics and Transcriptomics J. Biol. Chem., July 8, 2005; 280(27): 25590 - 25595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Piotrowski, A. Schemenewitz, A. Lopukhina, A. Muller, T. Janowitz, E. W. Weiler, and C. Oecking Desulfoglucosinolate Sulfotransferases from Arabidopsis thaliana Catalyze the Final Step in the Biosynthesis of the Glucosinolate Core Structure J. Biol. Chem., December 3, 2004; 279(49): 50717 - 50725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Saito Sulfur Assimilatory Metabolism. The Long and Smelling Road Plant Physiology, September 1, 2004; 136(1): 2443 - 2450. [Full Text] [PDF]
|
|
|
|
|
|
P. Jones, B. Messner, J.-I. Nakajima, A. R. Schaffner, and K. Saito UGT73C6 and UGT78D1, Glycosyltransferases Involved in Flavonol Glycoside Biosynthesis in Arabidopsis thaliana J. Biol. Chem., November 7, 2003; 278(45): 43910 - 43918. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |