|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 12, 10436-10442, March 21, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Insulin resistance in skeletal muscle is a
hallmark feature of type 2 diabetes. An increasing number of enzymes
and metabolic pathways have been implicated in the development of
insulin resistance. However, the primary cellular cause of insulin
resistance remains uncertain. Proteome analysis can quantitate a large
number of proteins and their post-translational modifications
simultaneously and is a powerful tool to study polygenic diseases like
type 2 diabetes. Using this approach on human skeletal muscle biopsies, we have identified eight potential protein markers for type 2 diabetes
in the fasting state. The observed changes in protein expression
indicate increased cellular stress, e.g. up-regulation of
two heat shock proteins, and perturbations in ATP (re)synthesis and
mitochondrial metabolism, e.g. down-regulation of ATP
synthase
Proteome Analysis Reveals Phosphorylation of ATP Synthase
-Subunit in Human Skeletal Muscle and Proteins with Potential
Roles in Type 2 Diabetes*
,,
,,, and§§
Diabetes Research Centre, Department
of Endocrinology, Odense University Hospital, DK-5000 Odense C
§ Centre for Proteome Analysis and ¶ Department of
Biochemistry and Molecular Biology, University of Southern Denmark,
DK-5230 Odense M, Copenhagen Muscle Research Centre, Department
of Medical Physiology and ** Department of Physiology,
Panum Institute, University of Copenhagen, N DK-2200 Copenhagen N,
and Novo Nordisk A/S,
DK-2880 Bagsværd, Denmark
-subunit and creatine kinase B, in skeletal muscle of
patients with type 2 diabetes. Phosphorylation appears to play a key,
potentially coordinating role for most of the proteins identified in
this study. In particular, we demonstrated that the catalytic
-subunit of ATP synthase is phosphorylated in vivo and
that the levels of a down-regulated ATP synthase -subunit
phosphoisoform in diabetic muscle correlated inversely with fasting
plasma glucose levels. These data suggest a role for phosphorylation of
ATP synthase -subunit in the regulation of ATP synthesis and that
alterations in the regulation of ATP synthesis and cellular stress
proteins may contribute to the pathogenesis of type 2 diabetes.
*
This work was supported in part by grants from the Danish
Research Agency (Technology by Highly Oriented Research) (9700893), the
Danish Diabetes Association, the Institute of Clinical Research, University of Southern Denmark, and the Danish National Research Foundation (504-14). Part of this work was performed in the frame of
the Danish Biotechnology Instrument Centre.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. L. Nordgaard, P. P. Karunadharma, X. Feng, T. W. Olsen, and D. A. Ferrington Mitochondrial Proteomics of the Retinal Pigment Epithelium at Progressive Stages of Age-Related Macular Degeneration Invest. Ophthalmol. Vis. Sci., July 1, 2008; 49(7): 2848 - 2855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H M De Feyter, N M A van den Broek, S F E Praet, K Nicolay, L J C van Loon, and J J Prompers Early or advanced stage type 2 diabetes is not accompanied by in vivo skeletal muscle mitochondrial dysfunction Eur. J. Endocrinol., May 1, 2008; 158(5): 643 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Hojlund, Z. Yi, H. Hwang, B. Bowen, N. Lefort, C. R. Flynn, P. Langlais, S. T. Weintraub, and L. J. Mandarino Characterization of the Human Skeletal Muscle Proteome by One-dimensional Gel Electrophoresis and HPLC-ESI-MS/MS Mol. Cell. Proteomics, February 1, 2008; 7(2): 257 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mei, C. Wood, M. R. L'Abbe, G. S. Gilani, G. M. Cooke, I. H. Curran, and C. W. Xiao Consumption of Soy Protein Isolate Modulates the Phosphorylation Status of Hepatic ATPase/ATP Synthase {beta} Protein and Increases ATPase Activity in Rats J. Nutr., September 1, 2007; 137(9): 2029 - 2035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Skov, D. Glintborg, S. Knudsen, T. Jensen, T. A. Kruse, Q. Tan, K. Brusgaard, H. Beck-Nielsen, and K. Hojlund Reduced Expression of Nuclear-Encoded Genes Involved in Mitochondrial Oxidative Metabolism in Skeletal Muscle of Insulin-Resistant Women With Polycystic Ovary Syndrome Diabetes, September 1, 2007; 56(9): 2349 - 2355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hu, X. He, K. A. Baggerly, K. R. Coombes, B. T.J. Hennessy, and G. B. Mills Non-parametric quantification of protein lysate arrays Bioinformatics, August 1, 2007; 23(15): 1986 - 1994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mogensen, K. Sahlin, M. Fernstrom, D. Glintborg, B. F. Vind, H. Beck-Nielsen, and K. Hojlund Mitochondrial Respiration Is Decreased in Skeletal Muscle of Patients With Type 2 Diabetes Diabetes, June 1, 2007; 56(6): 1592 - 1599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Arrell, S. T. Elliott, L. A. Kane, Y. Guo, Y. H. Ko, P. L. Pedersen, J. Robinson, M. Murata, A. M. Murphy, E. Marban, et al. Proteomic Analysis of Pharmacological Preconditioning: Novel Protein Targets Converge to Mitochondrial Metabolism Pathways Circ. Res., September 29, 2006; 99(7): 706 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Berria, L. Wang, D. K. Richardson, J. Finlayson, R. Belfort, T. Pratipanawatr, E. A. De Filippis, S. Kashyap, and L. J. Mandarino Increased collagen content in insulin-resistant skeletal muscle Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E560 - E565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M Scott, A. M Carter, and J. B. Findlay The application of proteomics to diabetes Diabetes and Vascular Disease Research, May 1, 2005; 2(2): 54 - 60. [Abstract] [PDF]
|
|
|
|
 |
|
 D. K. Richardson, S. Kashyap, M. Bajaj, K. Cusi, S. J. Mandarino, J. Finlayson, R. A. DeFronzo, C. P. Jenkinson, and L. J. Mandarino Lipid Infusion Decreases the Expression of Nuclear Encoded Mitochondrial Genes and Increases the Expression of Extracellular Matrix Genes in Human Skeletal Muscle J. Biol. Chem., March 18, 2005; 280(11): 10290 - 10297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, R. K. Wong, X. Wang, J. Moibi, M. J. Hessner, S. Greene, J. Wu, S. Sukumvanich, B. A. Wolf, and Z. Gao Leucine Culture Reveals That ATP Synthase Functions as a Fuel Sensor in Pancreatic {beta}-Cells J. Biol. Chem., December 24, 2004; 279(52): 53915 - 53923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Atalay, N. K. J. Oksala, D. E. Laaksonen, S. Khanna, C. Nakao, J. Lappalainen, S. Roy, O. Hanninen, and C. K. Sen Exercise training modulates heat shock protein response in diabetic rats J Appl Physiol, August 1, 2004; 97(2): 605 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fujinoki, T. Kawamura, T. Toda, H. Ohtake, N. Shimizu, S. Yamaoka, and M. Okuno Identification of the 58-kDa Phosphoprotein Associated with Motility Initiation of Hamster Spermatozoa J. Biochem., October 1, 2003; 134(4): 559 - 565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Schulenberg, R. Aggeler, J. M. Beechem, R. A. Capaldi, and W. F. Patton Analysis of Steady-state Protein Phosphorylation in Mitochondria Using a Novel Fluorescent Phosphosensor Dye J. Biol. Chem., July 11, 2003; 278(29): 27251 - 27255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Korc Diabetes Mellitus in the Era of Proteomics Mol. Cell. Proteomics, June 1, 2003; 2(6): 399 - 404. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |