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J. Biol. Chem., Vol. 278, Issue 13, 10885-10890, March 28, 2003
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From the Departamento de Bioquímica y Biología
Molecular, Universidad de Córdoba, Campus de Rabanales,
Edificio Severo Ochoa, Córdoba 14071, Spain
The molybdenum cofactor (Moco) is essential for
the activity of all molybdoenzymes except nitrogenase. The cDNA for
the Moco carrier protein (MocoCP) of Chlamydomonas
reinhardtii has been cloned by reverse transcription PCR
approaches with primers designed from microsequenced peptides of this
protein. The C. reinhardtii MocoCP has been expressed in
Escherichia coli. The recombinant protein has been
purified to electrophoretic homogeneity and is found assembled into a
homotetramer when Moco is not present under native conditions.
Recombinant MocoCP has the same biochemical characteristics as MocoCP
from C. reinhardtii, as it bound Moco from milk xanthine
oxidase with high affinity, prevented Moco inactivation by oxygen, and
transferred Moco efficiently to aponitrate reductase from the
Neurospora crassa nit1 mutant. The genomic DNA sequence
corresponding to the Chlamydomonas MocoCP gene,
CrMcp1, also was isolated. This gene contained three
introns in the coding region. The deduced amino acid sequence of
CrMcp1 did not show a significant identity to functionally
known proteins in the GenBankTM data base, although a
significant conservation was found with bacterial proteins of unknown
function. The results suggest that proteins having a Moco
binding function probably exist in other organisms.
Mcp1 Encodes the Molybdenum Cofactor Carrier Protein
in Chlamydomonas reinhardtii and Participates in
Protection, Binding, and Storage Functions of the Cofactor*
,
*
This work was supported in part by Grants
PB98-1022-CO-02 and BMC2002-03325 from the Dirección General de
Investigación Científica y Técnica, by the Junta de
Andalucía (to investigational group PAI CVI-128), and by the
Programa Propio de Ayudas a la Investigación of the University of
Córdoba.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Max Planck Institute for Plant Breeding Research,
Carl-von-Linne-Weg 10, Cologne 50858, Germany.
§
Present address: Departamento de Bioquímica,
Biología Molecular y Genética, Universidad de
Extremadura, Cáceres 10071, Spain.
¶
To whom correspondence should be addressed. Tel.:
34-957-218591; Fax: 34-957-218591; E-mail: bb1feree@uco.es.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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