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J. Biol. Chem., Vol. 278, Issue 13, 11320-11330, March 28, 2003

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Three-dimensional Models of Proteases Involved in Patterning of the Drosophila Embryo
CRUCIAL ROLE OF PREDICTED CATION BINDING SITES^*,

Thierry Rose, Ellen K. LeMosy^§, Angelene M. Cantwell, Dolly Banerjee-Roy, James B. Skeath^¶, and Enrico Di Cera

From the  Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, the ^§ Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, Georgia 30912, and the ^¶ Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110

Three-dimensional models of the catalytic domains of Nudel (Ndl), Gastrulation Defective (Gd), Snake (Snk), and Easter (Ea), and their complexes with substrate suggest a possible organization of the enzyme cascade controlling the dorsoventral fate of the fruit fly embryo. The models predict that Gd activates Snk, which in turn activates Ea. Gd can be activated either autoproteolytically or by Ndl. The three-dimensional models of each enzyme-substrate complex in the cascade rationalize existing mutagenesis data and the associated phenotypes. The models also predict unanticipated features like a Ca²⁺ binding site in Ea and a Na⁺ binding site in Ndl and Gd. These binding sites are likely to play a crucial role in vivo as suggested by mutant enzymes introduced into embryos as mRNAs. The mutations in Gd that eliminate Na⁺ binding cause an apparent increase in activity, whereas mutations in Ea that abrogate Ca²⁺ binding result in complete loss of activity. A mutation in Ea predicted to introduce Na⁺ binding results in apparently increased activity with ventralization of the embryo, an effect not observed with wild-type Ea mRNA.

E. D. C. dedicates this article to Professor Eraldo Antonini on the occasion of the 20th anniversary of his untimely death on March 19, 1983.

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