JBC Advanced Glycation Endproducts

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M209991200 on January 20, 2003

J. Biol. Chem., Vol. 278, Issue 13, 11376-11385, March 28, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/13/11376    most recent
M209991200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gurung, R.
Right arrow Articles by Mitchell, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gurung, R.
Right arrow Articles by Mitchell, C. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Identification of a Novel Domain in Two Mammalian Inositol-polyphosphate 5-Phosphatases That Mediates Membrane Ruffle Localization
THE INOSITOL 5-PHOSPHATASE SKIP LOCALIZES TO THE ENDOPLASMIC RETICULUM AND TRANSLOCATES TO MEMBRANE RUFFLES FOLLOWING EPIDERMAL GROWTH FACTOR STIMULATION*

Rajendra GurungDagger , April TanDagger , Lisa M. Ooms, Meagan J. McGrath, Richard D. Huysmans, Adam D. Munday, Mark Prescott, James C. Whisstock, and Christina A. Mitchell§

From the Department of Biochemistry and Molecular Biology, Monash University, Victoria 3800, Australia

SKIP (skeletal muscle and kidney enriched inositol phosphatase) is a recently identified phosphatidylinositol 3,4,5-trisphosphate- and phosphatidylinositol 4,5-bisphosphate-specific 5-phosphatase. In this study, we investigated the intracellular localization of SKIP. Indirect immunofluorescence and subcellular fractionation showed that, in serum-starved cells, both endogenous and recombinant SKIP colocalized with markers of the endoplasmic reticulum (ER). Following epidermal growth factor (EGF) stimulation, SKIP transiently translocated to plasma membrane ruffles and colocalized with submembranous actin. Data base searching demonstrated a novel 128-amino acid domain in the C terminus of SKIP, designated SKICH for SKIP carboxyl homology, which is also found in the 107-kDa 5-phosphatase PIPP and in members of the TRAF6-binding protein family. Recombinant SKIP lacking the SKICH domain localized to the ER, but did not translocate to membrane ruffles following EGF stimulation. The SKIP SKICH domain showed perinuclear localization and mediated EGF-stimulated plasma membrane ruffle localization. The SKICH domain of the 5-phosphatase PIPP also mediated plasma membrane ruffle localization. Mutational analysis identified the core sequence within the SKICH domain that mediated constitutive membrane association and C-terminal sequences unique to SKIP that contributed to ER localization. Collectively, these studies demonstrate a novel membrane-targeting domain that serves to recruit SKIP and PIPP to membrane ruffles.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Both authors contributed equally to this work.

§ To whom correspondence should be addressed: Dept. of Biochemistry and Molecular Biology, Monash University, Wellington Rd., Clayton, Victoria 3800, Australia. Tel.: 61-39-905-1245; Fax: 61-39-905-3726; E-mail: christina.mitchell@med.monash.edu.au.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
T. Ijuin, Y. E. Yu, K. Mizutani, A. Pao, S. Tateya, Y. Tamori, A. Bradley, and T. Takenawa
Increased Insulin Action in SKIP Heterozygous Knockout Mice
Mol. Cell. Biol., September 1, 2008; 28(17): 5184 - 5195.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K.-K. Ho, A. A. Anderson, E. Rosivatz, E. W.-F. Lam, R. Woscholski, and D. J. Mann
Identification of Cyclin A2 as the Downstream Effector of the Nuclear Phosphatidylinositol 4,5-Bisphosphate Signaling Network
J. Biol. Chem., February 29, 2008; 283(9): 5477 - 5485.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
B. Morriswood, G. Ryzhakov, C. Puri, S. D. Arden, R. Roberts, C. Dendrou, J. Kendrick-Jones, and F. Buss
T6BP and NDP52 are myosin VI binding partners with potential roles in cytokine signalling and cell adhesion
J. Cell Sci., August 1, 2007; 120(15): 2574 - 2585.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
D. Mason, G. V. Mallo, M. R. Terebiznik, B. Payrastre, B. B. Finlay, J. H. Brumell, L. Rameh, and S. Grinstein
Alteration of Epithelial Structure and Function Associated with PtdIns(4,5)P2 Degradation by a Bacterial Phosphatase
J. Gen. Physiol., March 26, 2007; 129(4): 267 - 283.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Lindsay, D. McCoull, L. Davidson, N. R. Leslie, A. Fairservice, A. Gray, J. Lucocq, and C. P. Downes
Localization of agonist-sensitive PtdIns(3,4,5)P3 reveals a nuclear pool that is insensitive to PTEN expression
J. Cell Sci., December 15, 2006; 119(24): 5160 - 5168.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
C. K. Yang, J. H. Kim, and M. R. Stallcup
Role of the N-Terminal Activation Domain of the Coiled-Coil Coactivator in Mediating Transcriptional Activation by {beta}-Catenin
Mol. Endocrinol., December 1, 2006; 20(12): 3251 - 3262.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. M. Ooms, C. G. Fedele, M. V. Astle, I. Ivetac, V. Cheung, R. B. Pearson, M. J. Layton, A. Forrai, H. H. Nandurkar, and C. A. Mitchell
The Inositol Polyphosphate 5-Phosphatase, PIPP, Is a Novel Regulator of Phosphoinositide 3-Kinase-dependent Neurite Elongation
Mol. Biol. Cell, February 1, 2006; 17(2): 607 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Faucherre, P. Desbois, F. Nagano, V. Satre, J. Lunardi, G. Gacon, and O. Dorseuil
Lowe syndrome protein Ocrl1 is translocated to membrane ruffles upon Rac GTPase activation: a new perspective on Lowe syndrome pathophysiology
Hum. Mol. Genet., June 1, 2005; 14(11): 1441 - 1448.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
I. Ivetac, A. D. Munday, M. V. Kisseleva, X.-M. Zhang, S. Luff, T. Tiganis, J. C. Whisstock, T. Rowe, P. W. Majerus, and C. A. Mitchell
The Type I{alpha} Inositol Polyphosphate 4-Phosphatase Generates and Terminates Phosphoinositide 3-Kinase Signals on Endosomes and the Plasma Membrane
Mol. Biol. Cell, May 1, 2005; 16(5): 2218 - 2233.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Nomura, H. Kanemura, T. Satoh, and T. Kataoka
Identification of a Novel Domain of Ras and Rap1 That Directs Their Differential Subcellular Localizations
J. Biol. Chem., May 21, 2004; 279(21): 22664 - 22673.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.