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J. Biol. Chem., Vol. 278, Issue 13, 11696-11704, March 28, 2003
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From the Department of Zoology and Genetics, Iowa State University,
Ames, Iowa 50011
Using a yeast two-hybrid screen we have
identified a novel isoform of the lola locus, Lola zf5,
that interacts with the chromosomal kinase JIL-1. We characterized the
lola locus and provide evidence that it is a complex locus
from which at least 17 different splice variants are likely to be
generated. Fifteen of these each have a different zinc finger
domain, whereas two are without. This potential for expression
of multiple gene products suggests that they serve diverse functional
roles in different developmental contexts. By Northern and Western blot
analyses we demonstrate that the expression of Lola zf5 is
developmentally regulated and that it is restricted to early
embryogenesis. Immunocytochemical labeling with a Lola zf5-specific
antibody of Drosophila embryos indicates that Lola zf5 is
localized to nuclei. Furthermore, by creating double-mutant flies we
show that a reduction of Lola protein levels resulting from mutations
in the lola locus acts as a dominant modifier of a
hypomorphic JIL-1 allele leading to an increase in
embryonic viability. Thus, genetic interaction assays provide direct
evidence that gene products from the lola locus function
within the same pathway as the chromosomal kinase JIL-1.
A Developmentally Regulated Splice Variant from the Complex
lola Locus Encoding Multiple Different Zinc Finger Domain
Proteins Interacts with the Chromosomal Kinase JIL-1*
*
This work was supported by National Institutes of Health
Grant GM62916 (to K. M. J.) and by a Stadler graduate fellowship award (to W. Z.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Zoology and
Genetics, 3154 Molecular Biology Bldg., Ames, IA 50011. Tel.: 515-294-7959; Fax: 515-294-4858; E-mail: kristen@iastate.edu.
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