|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 16, 14029-14036, April 18, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Homologous recombination is important for the
repair of double-stranded DNA breaks in all organisms. Rad51 and Rad54
proteins are two key components of the homologous recombination
machinery in eukaryotes. In vitro, Rad51 protein assembles
with single-stranded DNA to form the helical nucleoprotein
filament that promotes DNA strand exchange, a basic step of homologous
recombination. Rad54 protein interacts with this Rad51 nucleoprotein
filament and stimulates its DNA pairing activity, suggesting that Rad54
protein is a component of the nucleoprotein complex involved in the DNA
homology search. Here, using physical criteria, we demonstrate directly
the formation of Rad54-Rad51-DNA nucleoprotein co-complexes that
contain equimolar amounts of each protein. The binding of Rad54 protein
significantly stabilizes the Rad51 nucleoprotein filament formed on
either single-stranded DNA or double-stranded DNA. The Rad54-stabilized
nucleoprotein filament is more competent in DNA strand exchange and
acts over a broader range of solution conditions. Thus, the co-assembly of an interacting partner with the Rad51 nucleoprotein filament represents a novel means of stabilizing the biochemical entity central
to homologous recombination, and reveals a new function of Rad54 protein.
A Novel Function of Rad54 Protein
STABILIZATION OF THE Rad51 NUCLEOPROTEIN FILAMENT*
§, Department of Biochemistry, Drexel
University College of Medicine, Philadelphia, Pennsylvania 19102-1192 and the § Division of Biological Sciences, Sections of
Microbiology and of Molecular and Cellular Biology, University of
California, Davis, California 95616-8665
*
This work was supported by National Institutes of Health
Grant GM-62653 (to S. C. K.), Drexel University College of Medicine startup funds, and Pennsylvania Health Research Formula Funds from the
Tobacco Settlement Act (to A. V. M.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. C. Burgess, M. Lisby, V. Altmannova, L. Krejci, P. Sung, and R. Rothstein Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo J. Cell Biol., June 15, 2009; 185(6): 969 - 981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Haseltine and S. C. Kowalczykowski An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA Nucleic Acids Res., May 1, 2009; 37(8): 2757 - 2770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Nimonkar, R. A. Sica, and S. C. Kowalczykowski Rad52 promotes second-end DNA capture in double-stranded break repair to form complement-stabilized joint molecules PNAS, March 3, 2009; 106(9): 3077 - 3082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and W.-D. Heyer RAD54 controls access to the invading 3'-OH end after RAD51-mediated DNA strand invasion in homologous recombination in Saccharomyces cerevisiae Nucleic Acids Res., February 1, 2009; 37(2): 638 - 646. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rothenberg, J. M. Grimme, M. Spies, and T. Ha Human Rad52-mediated homology search and annealing occurs by continuous interactions between overlapping nucleoprotein complexes PNAS, December 23, 2008; 105(51): 20274 - 20279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Mazina and A. V. Mazin Human Rad54 protein stimulates human Mus81-Eme1 endonuclease PNAS, November 25, 2008; 105(47): 18249 - 18254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sarai, W. Kagawa, N. Fujikawa, K. Saito, J. Hikiba, K. Tanaka, K. Miyagawa, H. Kurumizaka, and S. Yokoyama Biochemical analysis of the N-terminal domain of human RAD54B Nucleic Acids Res., October 1, 2008; 36(17): 5441 - 5450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Rossi and A. V. Mazin Rad51 Protein Stimulates the Branch Migration Activity of Rad54 Protein J. Biol. Chem., September 5, 2008; 283(36): 24698 - 24706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Trickey, M. Grimaldi, and H. Yamano The Anaphase-Promoting Complex/Cyclosome Controls Repair and Recombination by Ubiquitylating Rhp54 in Fission Yeast Mol. Cell. Biol., June 15, 2008; 28(12): 3905 - 3916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wu, N. Kantake, T. Sugiyama, and S. C. Kowalczykowski Rad51 Protein Controls Rad52-mediated DNA Annealing J. Biol. Chem., May 23, 2008; 283(21): 14883 - 14892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Seong, M. G. Sehorn, I. Plate, I. Shi, B. Song, P. Chi, U. Mortensen, P. Sung, and L. Krejci Molecular Anatomy of the Recombination Mediator Function of Saccharomyces cerevisiae Rad52 J. Biol. Chem., May 2, 2008; 283(18): 12166 - 12174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Klutstein, H. Shaked, A. Sherman, N. Avivi-Ragolsky, E. Shema, D. Zenvirth, A. A. Levy, and G. Simchen Functional Conservation of the Yeast and Arabidopsis RAD54-Like Genes Genetics, April 1, 2008; 178(4): 2389 - 2397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Snowden, K.-S. Shim, C. Schmutte, S. Acharya, and R. Fishel hMSH4-hMSH5 Adenosine Nucleotide Processing and Interactions with Homologous Recombination Machinery J. Biol. Chem., January 4, 2008; 283(1): 145 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mine, L. Disseau, M. Takahashi, G. Cappello, M. Dutreix, and J.-L. Viovy Real-time measurements of the nucleation, growth and dissociation of single Rad51 DNA nucleoprotein filaments Nucleic Acids Res., December 18, 2007; 35(21): 7171 - 7187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Cullen, S. P. Hussey, C. Walker, J. Prudden, B.-Y. Wee, A. Dave, J. S. Findlay, A. P. Savory, and T. C. Humphrey Break-Induced Loss of Heterozygosity in Fission Yeast: Dual Roles for Homologous Recombination in Promoting Translocations and Preventing De Novo Telomere Addition Mol. Cell. Biol., November 1, 2007; 27(21): 7745 - 7757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Mazina, M. J. Rossi, N. H. Thomaa, and A. V. Mazin Interactions of Human Rad54 Protein with Branched DNA Molecules J. Biol. Chem., July 20, 2007; 282(29): 21068 - 21080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Chi, J. San Filippo, M. G. Sehorn, G. V. Petukhova, and P. Sung Bipartite stimulatory action of the Hop2-Mnd1 complex on the Rad51 recombinase Genes & Dev., July 15, 2007; 21(14): 1747 - 1757. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, X.-P. Zhang, J. A. Solinger, K. Kiianitsa, X. Yu, E. H. Egelman, and W.-D. Heyer Rad51 and Rad54 ATPase activities are both required to modulate Rad51-dsDNA filament dynamics Nucleic Acids Res., June 22, 2007; (2007) gkm412v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. W. Fung, G. S. Fortin, S. E. Peterson, and L. S. Symington The rad51-K191R ATPase-Defective Mutant Is Impaired for Presynaptic Filament Formation Mol. Cell. Biol., December 15, 2006; 26(24): 9544 - 9554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Otterlei, P. Bruheim, B. Ahn, W. Bussen, P. Karmakar, K. Baynton, and V. A. Bohr Werner syndrome protein participates in a complex with RAD51, RAD54, RAD54B and ATR in response to ICL-induced replication arrest J. Cell Sci., December 15, 2006; 119(24): 5137 - 5146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Symington and W.-D. Heyer Some disassembly required: role of DNA translocases in the disruption of recombination intermediates and dead-end complexes Genes & Dev., September 15, 2006; 20(18): 2479 - 2486. [Full Text] [PDF]
|
|
|
|
|
|
N. Sarai, W. Kagawa, T. Kinebuchi, A. Kagawa, K. Tanaka, K. Miyagawa, S. Ikawa, T. Shibata, H. Kurumizaka, and S. Yokoyama Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein Nucleic Acids Res., September 11, 2006; 34(16): 4429 - 4437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-D. Heyer, X. Li, M. Rolfsmeier, and X.-P. Zhang Rad54: the Swiss Army knife of homologous recombination? Nucleic Acids Res., September 10, 2006; 34(15): 4115 - 4125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. McCabe, N. C. Turner, C. J. Lord, K. Kluzek, A. Bialkowska, S. Swift, S. Giavara, M. J. O'Connor, A. N. Tutt, M. Z. Zdzienicka, et al. Deficiency in the Repair of DNA Damage by Homologous Recombination and Sensitivity to Poly(ADP-Ribose) Polymerase Inhibition Cancer Res., August 15, 2006; 66(16): 8109 - 8115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kiianitsa, J. A. Solinger, and W.-D. Heyer Terminal association of Rad54 protein with the Rad51-dsDNA filament PNAS, June 27, 2006; 103(26): 9767 - 9772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wesoly, S. Agarwal, S. Sigurdsson, W. Bussen, S. Van Komen, J. Qin, H. van Steeg, J. van Benthem, E. Wassenaar, W. M. Baarends, et al. Differential Contributions of Mammalian Rad54 Paralogs to Recombination, DNA Damage Repair, and Meiosis Mol. Cell. Biol., February 1, 2006; 26(3): 976 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-P. Zhang, K.-I. Lee, J. A. Solinger, K. Kiianitsa, and W.-D. Heyer Gly-103 in the N-terminal Domain of Saccharomyces cerevisiae Rad51 Protein Is Critical for DNA Binding J. Biol. Chem., July 15, 2005; 280(28): 26303 - 26311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ristic, M. Modesti, T. van der Heijden, J. van Noort, C. Dekker, R. Kanaar, and C. Wyman Human Rad51 filaments on double- and single-stranded DNA: correlating regular and irregular forms with recombination function Nucleic Acids Res., June 8, 2005; 33(10): 3292 - 3302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wolner and C. L. Peterson ATP-dependent and ATP-independent Roles for the Rad54 Chromatin Remodeling Enzyme during Recombinational Repair of a DNA Double Strand Break J. Biol. Chem., March 18, 2005; 280(11): 10855 - 10860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Raschle, S. Van Komen, P. Chi, T. Ellenberger, and P. Sung Multiple Interactions with the Rad51 Recombinase Govern the Homologous Recombination Function of Rad54 J. Biol. Chem., December 10, 2004; 279(50): 51973 - 51980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Mazina and A. V. Mazin Human Rad54 Protein Stimulates DNA Strand Exchange Activity of hRad51 Protein in the Presence of Ca2+ J. Biol. Chem., December 10, 2004; 279(50): 52042 - 52051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Alexiadis, A. Lusser, and J. T. Kadonaga A Conserved N-terminal Motif in Rad54 Is Important for Chromatin Remodeling and Homologous Strand Pairing J. Biol. Chem., June 25, 2004; 279(26): 27824 - 27829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Soustelle, L. Vernis, K. Freon, A. Reynaud-Angelin, R. Chanet, F. Fabre, and M. Heude A New Saccharomyces cerevisiae Strain with a Mutant Smt3-Deconjugating Ulp1 Protein Is Affected in DNA Replication and Requires Srs2 and Homologous Recombination for Its Viability Mol. Cell. Biol., June 15, 2004; 24(12): 5130 - 5143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Smirnova, S. Van Komen, P. Sung, and H. L. Klein Effects of Tumor-associated Mutations on Rad54 Functions J. Biol. Chem., June 4, 2004; 279(23): 24081 - 24088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sung, L. Krejci, S. Van Komen, and M. G. Sehorn Rad51 Recombinase and Recombination Mediators J. Biol. Chem., October 31, 2003; 278(44): 42729 - 42732. [Full Text] [PDF]
|
|
|
|
|
|
N. Adachi, H. Suzuki, S. Iiizumi, and H. Koyama Hypersensitivity of Nonhomologous DNA End-joining Mutants to VP-16 and ICRF-193: IMPLICATIONS FOR THE REPAIR OF TOPOISOMERASE II-MEDIATED DNA DAMAGE J. Biol. Chem., September 19, 2003; 278(38): 35897 - 35902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ogrunc and A. Sancar Identification and Characterization of Human MUS81-MMS4 Structure-specific Endonuclease J. Biol. Chem., June 6, 2003; 278(24): 21715 - 21720. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |