JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M209684200 on January 15, 2003

J. Biol. Chem., Vol. 278, Issue 16, 14461-14468, April 18, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/16/14461    most recent
M209684200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, B.
Right arrow Articles by Shore, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, B.
Right arrow Articles by Shore, G. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Uncleaved BAP31 in Association with A4 Protein at the Endoplasmic Reticulum Is an Inhibitor of Fas-initiated Release of Cytochrome c from Mitochondria*

Bing WangDagger §, Mai NguyenDagger §, David G. BreckenridgeDagger , Marina StojanovicDagger , Paul A. Clemons||, Stephan Kuppig**, and Gordon C. ShoreDagger Dagger Dagger

From the Dagger  Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y, Canada, the || Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, and the ** Department of Molecular Immunology, Biology III, University of Freiberg and the Max Planck Institute of Immunobiology, Freiburg 71908, Germany

BAP31 is a polytopic integral protein of the endoplasmic reticulum membrane and, like BID, is a preferred substrate of caspase-8. Upon Fas/CD95 stimulation, BAP31 is cleaved within its cytosolic domain, generating proapoptotic p20 BAP31. In human KB epithelial cells expressing the caspase-resistant mutant crBAP31, Fas stimulation resulted in cleavage of BID and insertion of BAX into mitochondrial membrane, but subsequent oligomerization of BAX and BAK, egress of cytochrome c to the cytosol, and apoptosis were impaired. Bap31-null mouse cells expressing crBAP31 cannot generate the endogenous p20 BAP31 cleavage product, yet crBAP31 conferred resistance to cellular condensation and cytochrome c release in response to activation of ectopic FKBPcasp8 by FK1012z. Full-length BAP31, therefore, is a direct inhibitor of these caspase-8-initiated events, acting independently of its ability to sequester p20, with which it interacts. Employing a novel split ubiquitin yeast two-hybrid screen for BAP31-interacting membrane proteins, the putative ion channel protein of the endoplasmic reticulum, A4, was detected and identified as a constitutive binding partner of BAP31 in human cells. Ectopic A4 that was introduced into A4-deficient cells cooperated with crBAP31 to resist Fas-induced egress of cytochrome c from mitochondria and cytoplasmic apoptosis.

* This work was supported in part by the National Cancer Institute of Canada, the Canadian Institutes of Health Research, the Deutsche Forchungsgemeinschaft through Grant SFB 388, and the Leibniz program.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

Recipient of a Canadian Institutes of Health doctoral research award.

Dagger Dagger To whom correspondence should be addressed: Dept. of Biochemistry, McIntyre Medical Sciences Bldg., McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec H3G 1Y6, Canada. Tel.: 514-398-7282; Fax: 514-398-7384; E-mail: gordon.shore@mcgill.ca.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. Szczesna-Skorupa and B. Kemper
BAP31 Is Involved in the Retention of Cytochrome P450 2C2 in the Endoplasmic Reticulum
J. Biol. Chem., February 17, 2006; 281(7): 4142 - 4148.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Stojanovic, M. Germain, M. Nguyen, and G. C. Shore
BAP31 and Its Caspase Cleavage Product Regulate Cell Surface Expression of Tetraspanins and Integrin-mediated Cell Survival
J. Biol. Chem., August 26, 2005; 280(34): 30018 - 30024.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Mathai, M. Germain, and G. C. Shore
BH3-only BIK Regulates BAX,BAK-dependent Release of Ca2+ from Endoplasmic Reticulum Stores and Mitochondrial Apoptosis during Stress-induced Cell Death
J. Biol. Chem., June 24, 2005; 280(25): 23829 - 23836.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. S. Maag, M. Mancini, A. Rosen, and C. E. Machamer
Caspase-resistant Golgin-160 Disrupts Apoptosis Induced by Secretory Pathway Stress and Ligation of Death Receptors
Mol. Biol. Cell, June 1, 2005; 16(6): 3019 - 3027.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
K. Iyer, L. Burkle, D. Auerbach, S. Thaminy, M. Dinkel, K. Engels, and I. Stagljar
Utilizing the Split-Ubiquitin Membrane Yeast Two-Hybrid System to Identify Protein-Protein Interactions of Integral Membrane Proteins
Sci. Signal., March 15, 2005; 2005(275): pl3 - pl3.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Obrdlik, M. El-Bakkoury, T. Hamacher, C. Cappellaro, C. Vilarino, C. Fleischer, H. Ellerbrok, R. Kamuzinzi, V. Ledent, D. Blaudez, et al.
K+ channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions
PNAS, August 17, 2004; 101(33): 12242 - 12247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. B. Gustafsson, J. G. Tsai, S. E. Logue, M. T. Crow, and R. A. Gottlieb
Apoptosis Repressor with Caspase Recruitment Domain Protects against Cell Death by Interfering with Bax Activation
J. Biol. Chem., May 14, 2004; 279(20): 21233 - 21238.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Wang, J. Pelletier, M. J. Massaad, A. Herscovics, and G. C. Shore
The Yeast Split-Ubiquitin Membrane Protein Two-Hybrid Screen Identifies BAP31 as a Regulator of the Turnover of Endoplasmic Reticulum-Associated Protein Tyrosine Phosphatase-Like B
Mol. Cell. Biol., April 1, 2004; 24(7): 2767 - 2778.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
I. Stagljar
Finding Partners: Emerging Protein Interaction Technologies Applied to Signaling Networks
Sci. Signal., December 16, 2003; 2003(213): pe56 - pe56.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.