JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M300991200 on February 12, 2003

J. Biol. Chem., Vol. 278, Issue 17, 15023-15034, April 25, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/17/15023    most recent
M300991200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berdiev, B. K.
Right arrow Articles by Benos, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berdiev, B. K.
Right arrow Articles by Benos, D. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Acid-sensing Ion Channels in Malignant Gliomas*

Bakhrom K. BerdievDagger , Jiazeng XiaDagger , Lee Anne McLeanDagger §, James M. MarkertDagger , G. Yancey Gillespie, Timothy B. Mapstone||, Anjaparavanda P. NarenDagger **, Biljana JovovDagger , James K. BubienDagger , Hong-Long JiDagger , Catherine M. FullerDagger , Kevin L. KirkDagger , and Dale J. BenosDagger Dagger Dagger

From the Departments of Dagger  Physiology and Biophysics and  Surgery, University of Alabama at Birmingham, Birmingham, Alabama 35294 and the || Department of Neurosurgery, Emory University, Atlanta, Georgia 30322

High grade glioma cells derived from patient biopsies express an amiloride-sensitive sodium conductance that has properties attributed to the human brain sodium channel family, also known as acid-sensing ion channels (ASICs). This amiloride-sensitive conductance was not detected in cells obtained from normal brain tissue or low grade or benign tumors. Differential gene profiling data showed that ASIC1 and ASIC2 mRNA were present in normal and low grade tumor cells. Although ASIC1 was present in all of the high grade glial cells examined, ASIC2 mRNA was detected in less than half. The main purpose of our work was to examine the molecular mechanisms that may underlie the constitutively activated sodium currents present in high grade glioma cells. Our results show that 1) gain-of-function mutations of ASIC1 were not present in a number of freshly resected and cultured high grade gliomas, 2) syntaxin 1A inhibited ASIC currents only when ASIC1 and ASIC2 were co-expressed, and 3) the inhibition of ASIC currents by syntaxin 1A had an absolute requirement for either gamma - or delta -hENaC. Transfection of cultured cells originally derived from high grade gliomas (U87-MG and SK-MG1) with ASIC2 abolished basal amiloride-sensitive sodium conductance; this inhibition was reversed by dialysis of the cell interior with Munc-18, a syntaxin-binding protein that typically blocks the interaction of syntaxin with other proteins. Thus, syntaxin 1A cannot inhibit Na+ permeability in the absence of adequate plasma membrane ASIC2 expression, accounting for the observed functional expression of amiloride-sensitive currents in high grade glioma cells.

* This work was supported by National Institutes of Health Grant CA71933 and by funds from the Brain Tumor Foundation for Children.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Present address: Novartis Pharmaceuticals Corporation, Gaithersburg, MD 20878.

** Present address: University of Tennessee Health Science Center, Department of Physiology, Memphis, TN 38163.

Dagger Dagger To whom correspondence should be addressed: Dept. of Physiology and Biophysics, The University of Alabama at Birmingham, 1918 University Blvd., MCLM 704, Birmingham, AL 35294-0005. Tel.: 205-934-6220; Fax: 205-934-2377; E-mail: benos@physiology.uab.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. C. Grifoni, S. E. McKey, and H. A. Drummond
Hsc70 regulates cell surface ASIC2 expression and vascular smooth muscle cell migration
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2022 - H2030.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C. M. Fuller and D. J. Benos
Putting the brakes on vascular smooth muscle cell migration
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H1987 - H1988.
[Full Text] [PDF]

Home page
 HypertensionHome page
H. A. Drummond, N. L. Jernigan, and S. C. Grifoni
Sensing Tension: Epithelial Sodium Channel/Acid-Sensing Ion Channel Proteins in Cardiovascular Homeostasis
Hypertension, May 1, 2008; 51(5): 1265 - 1271.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. P. Gannon, L. G. VanLandingham, N. L. Jernigan, S. C. Grifoni, G. Hamilton, and H. A. Drummond
Impaired pressure-induced constriction in mouse middle cerebral arteries of ASIC2 knockout mice
Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1793 - H1803.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
H. A. Drummond, S. C. Grifoni, and N. L. Jernigan
A New Trick for an Old Dogma: ENaC Proteins as Mechanotransducers in Vascular Smooth Muscle
Physiology, February 1, 2008; 23(1): 23 - 31.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J.-H. Cho and C. C. Askwith
Presynaptic Release Probability Is Increased in Hippocampal Neurons From ASIC1 Knockout Mice
J Neurophysiol, February 1, 2008; 99(2): 426 - 441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. H. Vila-Carriles, Z.-H. Zhou, J. K. Bubien, C. M. Fuller, and D. J. Benos
Participation of the Chaperone Hsc70 in the Trafficking and Functional Expression of ASIC2 in Glioma Cells
J. Biol. Chem., November 23, 2007; 282(47): 34381 - 34391.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. B. Ross, C. M. Fuller, J. K. Bubien, and D. J. Benos
Amiloride-sensitive Na+ channels contribute to regulatory volume increases in human glioma cells
Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1181 - C1185.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. H. Meltzer, N. Kapoor, Y. J. Qadri, S. J. Anderson, C. M. Fuller, and D. J. Benos
Heteromeric Assembly of Acid-sensitive Ion Channel and Epithelial Sodium Channel Subunits
J. Biol. Chem., August 31, 2007; 282(35): 25548 - 25559.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Su, Q. Li, K. Shrestha, E. Cormet-Boyaka, L. Chen, P. R. Smith, E. J. Sorscher, D. J. Benos, S. Matalon, and H.-L. Ji
Interregulation of Proton-gated Na+ Channel 3 and Cystic Fibrosis Transmembrane Conductance Regulator
J. Biol. Chem., December 1, 2006; 281(48): 36960 - 36968.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. H. Vila-Carriles, G. G. Kovacs, B. Jovov, Z.-H. Zhou, A. K. Pahwa, G. Colby, O. Esimai, G. Y. Gillespie, T. B. Mapstone, J. M. Markert, et al.
Surface Expression of ASIC2 Inhibits the Amiloride-sensitive Current and Migration of Glioma Cells
J. Biol. Chem., July 14, 2006; 281(28): 19220 - 19232.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. S. Adams, K. R. Robinson, T. Fukumoto, S. Yuan, R. C. Albertson, P. Yelick, L. Kuo, M. McSweeney, and M. Levin
Early, H+-V-ATPase-dependent proton flux is necessary for consistent left-right patterning of non-mammalian vertebrates
Development, May 1, 2006; 133(9): 1657 - 1671.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Price, R. J. Thompson, J. O. Eshcol, J. A. Wemmie, and C. J. Benson
Stomatin Modulates Gating of Acid-sensing Ion Channels
J. Biol. Chem., December 17, 2004; 279(51): 53886 - 53891.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. K. Bubien, H.-L. Ji, G. Y. Gillespie, C. M. Fuller, J. M. Markert, T. B. Mapstone, and D. J. Benos
Cation selectivity and inhibition of malignant glioma Na+ channels by Psalmotoxin 1
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1282 - C1291.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-L. Ji and D. J. Benos
Degenerin Sites Mediate Proton Activation of {delta}{beta}{gamma}-Epithelial Sodium Channel
J. Biol. Chem., June 25, 2004; 279(26): 26939 - 26947.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. K. Berdiev, B. Jovov, W. C. Tucker, A. P. Naren, C. M. Fuller, E. R. Chapman, and D. J. Benos
ENaC subunit-subunit interactions and inhibition by syntaxin 1A
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1100 - F1106.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. C. Askwith, J. A. Wemmie, M. P. Price, T. Rokhlina, and M. J. Welsh
Acid-sensing Ion Channel 2 (ASIC2) Modulates ASIC1 H+-activated Currents in Hippocampal Neurons
J. Biol. Chem., April 30, 2004; 279(18): 18296 - 18305.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.