|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 17, 15239-15245, April 25, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the
Identification of Phospholipid Scramblase 1 as a Novel
Interacting Molecule with
-Secretase (-Site Amyloid Precursor
Protein (APP) Cleaving Enzyme (BACE))*
,,,,,,
,, and**
Department of Cell Biology and Neuroscience
A1, Osaka University Graduate School of Medicine, Yamadaoka 2-2, Suita, Osaka 565-0871, Japan, § Molecular and
Experimental Medicine MEM275, The Scripps Research Institute, La
Jolla, California 92037, and the Department of Molecular
Biodynamics, The Tokyo Metropolitan Institute of Medical Science
(RINSHOKEN), Bunkyo-ku Tokyo 113-8613, Japan
-Site amyloid precursor protein (APP)-cleaving
enzyme (BACE) is an integral membrane aspartic proteinase
responsible for -site processing of APP, and its cytoplasmic region
composed of 24 amino acid residues has been shown to be involved in the endosomal localization of BACE. With the yeast two-hybrid screening, we
found that the cytoplasmic domain of phospholipid scramblase 1 (PLSCR1), a type II integral membrane protein, interacts with the
cytoplasmic region of BACE. In cultured cells, BACE and PLSCR1 were
colocalized in the Golgi area and in endosomal compartments, whereas
they were co-redistributed in late endosome-derived multivesicular bodies when treated with U18666A, suggesting that both proteins share a
common trafficking pathway in cells. Co-immunoprecipitation analysis
showed that both proteins form a protein complex at an endogenous
expression level in the human neuroblastoma SH-SY5Ycells, and
the dileucine residue of the BACE tail is also revealed to be essential
for the physical interaction with PLSCR1 in vitro and
in vivo. Moreover, both BACE and PLSCR1 were localized in a
low buoyant lipid microdomain in SH-SY5Y cells. The dileucine-defective BACE mutant was also fractionated into the lipid microdomain, but much
less stably than wild-type BACE. Taken together, our current study
suggests the functional involvement of PLSCR1 in the intracellular
distribution of BACE and/or recruitment of BACE into the
detergent-insoluble lipid raft.
*
This work was supported by a grant-in-aid for Scientific
Research on Priority Areas, Advanced Brain Science Project, from the
Ministry of Education, Culture, Sports and Science and Technology, Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. K. Lakshmana, I.-S. Yoon, E. Chen, E. Bianchi, E. H. Koo, and D. E. Kang Novel Role of RanBP9 in BACE1 Processing of Amyloid Precursor Protein and Amyloid {beta} Peptide Generation J. Biol. Chem., May 1, 2009; 284(18): 11863 - 11872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ariga, M. P. McDonald, and R. K. Yu Thematic Review Series: Sphingolipids. Role of ganglioside metabolism in the pathogenesis of Alzheimer's disease--a review J. Lipid Res., June 1, 2008; 49(6): 1157 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Smrz, P. Lebduska, L. Draberova, J. Korb, and P. Draber Engagement of Phospholipid Scramblase 1 in Activated Cells: IMPLICATION FOR PHOSPHATIDYLSERINE EXTERNALIZATION AND EXOCYTOSIS J. Biol. Chem., April 18, 2008; 283(16): 10904 - 10918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Wyles, Z. Wu, S. E.L. Mirski, and S. P.C. Cole Nuclear interactions of topoisomerase II {alpha} and {beta} with phospholipid scramblase 1 Nucleic Acids Res., June 12, 2007; (2007) gkm434v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Acharya, M. B. Edwards, R. A. Jorquera, H. Silva, K. Nagashima, P. Labarca, and J. K. Acharya Drosophila melanogaster Scramblases modulate synaptic transmission. J. Cell Biol., April 10, 2006; 173(1): 69 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Vetrivel, H. Cheng, S.-H. Kim, Y. Chen, N. Y. Barnes, A. T. Parent, S. S. Sisodia, and G. Thinakaran Spatial Segregation of {gamma}-Secretase and Substrates in Distinct Membrane Domains J. Biol. Chem., July 8, 2005; 280(27): 25892 - 25900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. F. von Arnim, A. Kinoshita, I. D. Peltan, M. M. Tangredi, L. Herl, B. M. Lee, R. Spoelgen, T. T. Hshieh, S. Ranganathan, F. D. Battey, et al. The Low Density Lipoprotein Receptor-related Protein (LRP) Is a Novel {beta}-Secretase (BACE1) Substrate J. Biol. Chem., May 6, 2005; 280(18): 17777 - 17785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-P. Huang, W.-P. Chang, G. Koelsch, R. T. Turner III, F. Lupu, and J. Tang Internalization of Exogenously Added Memapsin 2 ({beta}-Secretase) Ectodomain by Cells Is Mediated by Amyloid Precursor Protein J. Biol. Chem., September 3, 2004; 279(36): 37886 - 37894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fukumoto, D. L. Rosene, M. B. Moss, S. Raju, B. T. Hyman, and M. C. Irizarry {beta}-Secretase Activity Increases with Aging in Human, Monkey, and Mouse Brain Am. J. Pathol., February 1, 2004; 164(2): 719 - 725. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |