| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 18, 15771-15777, May 2, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Biology, Technion-Israel Institute of
Technology, Haifa 32000, Israel
The mechanism of RNA degradation
in Escherichia coli involves endonucleolytic cleavage,
polyadenylation of the cleavage product by poly(A) polymerase, and
exonucleolytic degradation by the exoribonucleases, polynucleotide
phosphorylase (PNPase) and RNase II. The poly(A) tails are homogenous,
containing only adenosines in most of the growth conditions. In the
chloroplast, however, the same enzyme, PNPase, polyadenylates and
degrades the RNA molecule; there is no equivalent for the E. coli poly(A) polymerase enzyme. Because cyanobacteria is a
prokaryote believed to be related to the evolutionary ancestor of the
chloroplast, we asked whether the molecular mechanism of RNA
polyadenylation in the Synechocystis PCC6803 cyanobacteria is similar to that in E. coli or the chloroplast. We found
that RNA polyadenylation in Synechocystis is similar to
that in the chloroplast but different from E. coli. No
poly(A) polymerase enzyme exists, and polyadenylation is performed by
PNPase, resulting in heterogeneous poly(A)-rich tails. These
heterogeneous tails were found in the amino acid coding region, the 5'
and 3' untranslated regions of mRNAs, as well as in rRNA and the
single intron located at the tRNAfmet. Furthermore, unlike
E. coli, the inactivation of PNPase or RNase II genes
caused lethality. Together, our results show that the RNA
polyadenylation and degradation mechanisms in cyanobacteria and
chloroplast are very similar to each other but different from E. coli.
RNA Polyadenylation and Degradation in Cyanobacteria Are Similar
to the Chloroplast but Different from Escherichia
coli*
*
This work was supported by grants from the Israel Science
Foundation and the Israel-USA Binational Agriculture Research and Development Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 972-4-8293171;
Fax: 972-4-8295587; E-mail: gadis@tx.technion.ac.il.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Schein, S. Sheffy-Levin, F. Glaser, and G. Schuster The RNase E/G-type endoribonuclease of higher plants is located in the chloroplast and cleaves RNA similarly to the E. coli enzyme RNA, June 1, 2008; 14(6): 1057 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Zimmer, Z. Fei, and D. B. Stern Genome-Based Analysis of Chlamydomonas reinhardtii Exoribonucleases and Poly(A) Polymerases Predicts Unexpected Organellar and Exosomal Features Genetics, May 1, 2008; 179(1): 125 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Slomovic and G. Schuster Stable PNPase RNAi silencing: Its effect on the processing and adenylation of human mitochondrial RNA RNA, February 1, 2008; 14(2): 310 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Portnoy, G. Palnizky, S. Yehudai-Resheff, F. Glaser, and G. Schuster Analysis of the human polynucleotide phosphorylase (PNPase) reveals differences in RNA binding and response to phosphate compared to its bacterial and chloroplast counterparts RNA, February 1, 2008; 14(2): 297 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Chang, M. Cozad, G. A. Mackie, and G. H. Jones Kinetics of Polynucleotide Phosphorylase: Comparison of Enzymes from Streptomyces and Escherichia coli and Effects of Nucleoside Diphosphates J. Bacteriol., January 1, 2008; 190(1): 98 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Martin and W. Keller RNA-specific ribonucleotidyl transferases RNA, November 1, 2007; 13(11): 1834 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Mohanty and S. R. Kushner The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells Nucleic Acids Res., November 14, 2006; 34(19): 5695 - 5704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Portnoy and G. Schuster RNA polyadenylation and degradation in different Archaea; roles of the exosome and RNase R Nucleic Acids Res., November 6, 2006; 34(20): 5923 - 5931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Slomovic, D. Laufer, D. Geiger, and G. Schuster Polyadenylation of ribosomal RNA in human cells Nucleic Acids Res., May 31, 2006; 34(10): 2966 - 2975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bralley, B. Gust, S. Chang, K. F. Chater, and G. H. Jones RNA 3'-tail synthesis in Streptomyces: in vitro and in vivo activities of RNase PH, the SCO3896 gene product and polynucleotide phosphorylase. Microbiology, March 1, 2006; 152(Pt 3): 627 - 636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and D. Morse Rampant polyuridylylation of plastid gene transcripts in the dinoflagellate Lingulodinium Nucleic Acids Res., January 24, 2006; 34(2): 613 - 619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bralley, S. A. Chang, and G. H. Jones A Phylogeny of Bacterial RNA Nucleotidyltransferases: Bacillus halodurans Contains Two tRNA Nucleotidyltransferases J. Bacteriol., September 1, 2005; 187(17): 5927 - 5936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Slomovic, D. Laufer, D. Geiger, and G. Schuster Polyadenylation and Degradation of Human Mitochondrial RNA: the Prokaryotic Past Leaves Its Mark Mol. Cell. Biol., August 1, 2005; 25(15): 6427 - 6435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Campos-Guillen, P. Bralley, G. H. Jones, D. H. Bechhofer, and G. Olmedo-Alvarez Addition of Poly(A) and Heteropolymeric 3' Ends in Bacillus subtilis Wild-Type and Polynucleotide Phosphorylase-Deficient Strains J. Bacteriol., July 15, 2005; 187(14): 4698 - 4706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nagaike, T. Suzuki, T. Katoh, and T. Ueda Human Mitochondrial mRNAs Are Stabilized with Polyadenylation Regulated by Mitochondria-specific Poly(A) Polymerase and Polynucleotide Phosphorylase J. Biol. Chem., May 20, 2005; 280(20): 19721 - 19727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Tomecki, A. Dmochowska, K. Gewartowski, A. Dziembowski, and P. P. Stepien Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase Nucleic Acids Res., November 16, 2004; 32(20): 6001 - 6014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. MARTIN and W. KELLER Sequence motifs that distinguish ATP(CTP):tRNA nucleotidyl transferases from eubacterial poly(A) polymerases RNA, June 1, 2004; 10(6): 899 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bralley and G. H. Jones Organization and Expression of the Polynucleotide Phosphorylase Gene (pnp) of Streptomyces: Processing of pnp Transcripts in Streptomyces antibioticus J. Bacteriol., May 15, 2004; 186(10): 3160 - 3172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Sohlberg, J. Huang, and S. N. Cohen The Streptomyces coelicolor Polynucleotide Phosphorylase Homologue, and Not the Putative Poly(A) Polymerase, Can Polyadenylate RNA J. Bacteriol., December 15, 2003; 185(24): 7273 - 7278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yehudai-Resheff, V. Portnoy, S. Yogev, N. Adir, and G. Schuster Domain Analysis of the Chloroplast Polynucleotide Phosphorylase Reveals Discrete Functions in RNA Degradation, Polyadenylation, and Sequence Homology with Exosome Proteins PLANT CELL, September 1, 2003; 15(9): 2003 - 2019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bralley and G. H. Jones Overexpression of the polynucleotide phosphorylase gene (pnp) of Streptomyces antibioticus affects mRNA stability and poly(A) tail length but not ppGpp levels Microbiology, August 1, 2003; 149(8): 2173 - 2182. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
