HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 19, 16543-16550, May 9, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/19/16543    most recent M300548200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Engblom, A. C. Articles by Kristiansen, U. Search for Related Content PubMed PubMed Citation Articles by Engblom, A. C. Articles by Kristiansen, U. Social Bookmarking                      What's this?

Actions and Interactions of Extracellular Potassium and Kainate on Expression of 13 -Aminobutyric Acid Type A Receptor Subunits in Cultured Mouse Cerebellar Granule Neurons^*

A. Christine Engblom^§, Flemming F. Johansen^¶, and Uffe Kristiansen

From the  Department of Pharmacology, Royal Danish School of Pharmacy, Copenhagen 2100, Denmark and ^¶ Laboratory of Neuropathology, University of Copenhagen, Copenhagen 2100, Denmark

Cerebellar granule neurons in culture are a popular model for studying neuronal signaling and development. Depolarizing concentrations of K⁺ are routinely used to enhance cell survival, and kainate is sometimes added to eliminate GABAergic neurons. We have investigated the effect of these measures on expression of mRNA for -aminobutyric acid type A (GABA_A) receptor 1-6, 1-3, 1-3, and  subunits in cultures of mouse cerebellar granule neurons grown for 7 or 12 days in vitro (DIV) using semiquantitative reverse transcription-PCR. We detected mRNA for the 1, 2, 5, 6, 2, 3, 2, and  subunits in all the cell cultures, but the expression levels of the 5-, 6-, and 2-subunit mRNAs were significantly dependent on the composition of the culture medium. Both an increase of the extracellular K⁺ concentration from 5 to 25 mM and the addition of 50 µM kainate immediately depolarized the neurons but prolonged exposure (7-8 DIV)-induced compensatory hyperpolarization. 25 mM K⁺ caused a shift from 6 to 5 expression measured at 7 and 12 DIV, which was mimicked by kainate in 12 DIV cultures. The expression of 2 was decreased by 25 mM K⁺ in 7 DIV cultures and by kainate in 12 DIV cultures. The effects on 2 expression could not be ascribed to depolarization. Alterations of 6 mRNA expression were reflected in altered sensitivity to GABA and furosemide of the resulting receptors. Our study has shown that a depolarizing K⁺ concentration as well as kainate in the culture medium significantly disturbs maturation of GABA_A receptor subunit expression.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				U. Strauss, F.-W. Zhou, J. Henning, A. Battefeld, A. Wree, R. Kohling, S. J.-P. Haas, R. Benecke, A. Rolfs, and U. Gimsa Increasing Extracellular Potassium Results in Subthalamic Neuron Activity Resembling That Seen in a 6-Hydroxydopamine Lesion J Neurophysiol, June 1, 2008; 99(6): 2902 - 2915. [Abstract] [Full Text] [PDF]

				M. Zanzouri, I. Lauritzen, F. Duprat, M. Mazzuca, F. Lesage, M. Lazdunski, and A. Patel Membrane Potential-regulated Transcription of the Resting K+ Conductance TASK-3 via the Calcineurin Pathway J. Biol. Chem., September 29, 2006; 281(39): 28910 - 28918. [Abstract] [Full Text] [PDF]