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Originally published In Press as doi:10.1074/jbc.M301995200 on March 5, 2003

J. Biol. Chem., Vol. 278, Issue 19, 16561-16566, May 9, 2003
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SIC, a Secreted Protein of Streptococcus pyogenes That Inactivates Antibacterial Peptides*

Inga-Maria FrickDagger §, Per Åkesson, Magnus RasmussenDagger , Artur Schmidtchen, and Lars BjörckDagger

From the Dagger  Section for Molecular Pathogenesis, Department of Cell and Molecular Biology, Lund University, and the  Department of Medical Microbiology, Dermatology, and Infection, Lund University Hospital, S-221 85 Lund, Sweden

Some isolates of the significant human pathogen Streptococcus pyogenes, including virulent strains of the M1 serotype, secrete protein SIC. This molecule, secreted in large quantities, interferes with complement function. As a result of natural selection, SIC shows a high degree of variation. Here we provide a plausible explanation for this variation and the fact that strains of the M1 serotype are the most frequent cause of severe invasive S. pyogenes infections. Thus, protein SIC was found to inactivate human neutrophil alpha -defensin and LL-37, two major antibacterial peptides involved in bacterial clearance. This inactivation protected S. pyogenes against the antibacterial effect of the peptides. Moreover, SIC isolated from S. pyogenes of the M1 serotype was more powerful in this respect than SIC variants from strains of M serotypes 12 and 55, serotypes rarely connected with invasive infections.

* This work was supported by grants from the Swedish Research Council (Projects 7480, 13471, and 14379), the Foundations of Crafoord, Bergvall, and Österlund, and the Royal Physiographic Society.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Cell and Molecular Biology, Lund University, BMC, B14, Tornavägen 10, S-221 84 Lund, Sweden. Tel.: 46-46-222-8569; Fax: 46-46-157756; E-mail: Inga-Maria.Frick@medkem.lu.se.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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