Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M301028200 on March 3, 2003

J. Biol. Chem., Vol. 278, Issue 19, 16857-16862, May 9, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/19/16857    most recent
M301028200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chung, J. W.
Right arrow Articles by Kim, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chung, J. W.
Right arrow Articles by Kim, K. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

37-kDa Laminin Receptor Precursor Modulates Cytotoxic Necrotizing Factor 1-mediated RhoA Activation and Bacterial Uptake*

Jin Woong ChungDagger , Suk Jin Hong§, Kee Jun KimDagger , Daniel GotiDagger , Monique F. StinsDagger , Sooan ShinDagger , Valina L. Dawson§||**, Ted M. Dawson§**, and Kwang Sik KimDagger Dagger Dagger

From the Departments of Dagger  Pediatrics, § Neurology,  Neuroscience, || Physiology, and ** Institute for Cell Engineering, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21287

Cytotoxic necrotizing factor 1 (CNF1) is a bacterial toxin known to activate Rho GTPases and induce host cell cytoskeleton rearrangements. The constitutive activation of Rho GTPases by CNF1 is shown to enhance bacterial uptake in epithelial cells and human brain microvascular endothelial cells. However, it is unknown how exogenous CNF1 exhibits such phenotypes in eukaryotic cells. Here, we identified 37-kDa laminin receptor precursor (LRP) as the receptor for CNF1 from screening the cDNA library of human brain microvascular endothelial cells by the yeast two-hybrid system using the N-terminal domain of CNF1 as bait. CNF1-mediated RhoA activation and bacterial uptake were inhibited by exogenous LRP or LRP antisense oligodeoxynucleotides, whereas they were increased in LRP-overexpressing cells. These findings indicate that the CNF1 interaction with LRP is the initial step required for CNF1-mediated RhoA activation and bacterial uptake in eukaryotic cells.

* This work was supported by grants from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Dagger To whom correspondence should be addressed: Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 600 N. Wolfe St., Park 256, Baltimore, MD 21287. Tel.: 410-614-3917; Fax: 410-614-1491; E-mail: kwangkim@jhmi.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
K. K. Grande, K. C. Meysick, S. B. Rasmussen, and A. D. O'Brien
Cytotoxic Necrotizing Factor Type 1-Neutralizing Monoclonal Antibody NG8 Recognizes Three Amino Acids in a C-Terminal Region of the Toxin and Reduces Toxin Binding to HEp-2 Cells
Infect. Immun., January 1, 2009; 77(1): 170 - 179.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
S. M. Jacobsen, D. J. Stickler, H. L. T. Mobley, and M. E. Shirtliff
Complicated Catheter-Associated Urinary Tract Infections Due to Escherichia coli and Proteus mirabilis
Clin. Microbiol. Rev., January 1, 2008; 21(1): 26 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. A. McNichol, S. B. Rasmussen, H. M. Carvalho, K. C. Meysick, and A. D. O'Brien
Two Domains of Cytotoxic Necrotizing Factor Type 1 Bind the Cellular Receptor, Laminin Receptor Precursor Protein
Infect. Immun., November 1, 2007; 75(11): 5095 - 5104.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
B. Blumenthal, C. Hoffmann, K. Aktories, S. Backert, and G. Schmidt
The Cytotoxic Necrotizing Factors from Yersinia pseudotuberculosis and from Escherichia coli Bind to Different Cellular Receptors but Take the Same Route to the Cytosol
Infect. Immun., July 1, 2007; 75(7): 3344 - 3353.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
J. M. Davis, H. M. Carvalho, S. B. Rasmussen, and A. D. O'Brien
Cytotoxic Necrotizing Factor Type 1 Delivered by Outer Membrane Vesicles of Uropathogenic Escherichia coli Attenuates Polymorphonuclear Leukocyte Antimicrobial Activity and Chemotaxis.
Infect. Immun., August 1, 2006; 74(8): 4401 - 4408.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Qiao, F. Huang, R. P. Naikawadi, K. S. Kim, T. Said, and H. Lum
Lysophosphatidylcholine impairs endothelial barrier function through the G protein-coupled receptor GPR4
Am J Physiol Lung Cell Mol Physiol, July 1, 2006; 291(1): L91 - L101.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. J. Kim, J. W. Chung, and K. S. Kim
67-kDa Laminin Receptor Promotes Internalization of Cytotoxic Necrotizing Factor 1-expressing Escherichia coli K1 into Human Brain Microvascular Endothelial Cells
J. Biol. Chem., January 14, 2005; 280(2): 1360 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
C. Zeng, D. Wang, Z. Yang, Z. Wang, L. D. Asico, C. S. Wilcox, G. M. Eisner, W. J. Welch, R. A. Felder, and P. A. Jose
Dopamine D1 Receptor Augmentation of D3 Receptor Action in Rat Aortic or Mesenteric Vascular Smooth Muscles
Hypertension, March 1, 2004; 43(3): 673 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
D. A. E. Cochran, C. A. Evans, D. Blinco, J. Burthem, F. K. Stevenson, S. J. Gaskell, and A. D. Whetton
Proteomic Analysis of Chronic Lymphocytic Leukemia Subtypes with Mutated or Unmutated Ig VH Genes
Mol. Cell. Proteomics, December 1, 2003; 2(12): 1331 - 1341.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement