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J. Biol. Chem., Vol. 278, Issue 19, 17000-17005, May 9, 2003
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From the Department of Physiology II, University of Freiburg,
Hermann-Herder-Strasse 7, 79104 Freiburg, Germany
Traffic of integral membrane proteins
along the secretory pathway is not simply a default process but can be
selective. Such selectivity is achieved by sequence information within
the cargo protein that recruits coat protein complexes to drive the
formation of transport vesicles. A number of sequence motifs have been
identified in the cytoplasmic domains of ion channels that regulate
early trafficking events between the endoplasmic reticulum and the
Golgi complex. Here, we demonstrate that the following trafficking step from the Golgi compartment to the plasma membrane can also be selective. The N-terminal domain of the inward rectifier potassium channel Kir2.1 contains specific sequence information that is necessary
for its efficient export from the Golgi complex. Lack of this
information results in accumulation of the protein within the Golgi and
a significant decrease in cell surface expression. As similar results
were obtained for the N terminus of another Kir channel subfamily
member, Kir4.1, which could functionally substitute for the Kir2.1 N
terminus, we propose a more general role of the identified N-terminal
domains for post-Golgi trafficking of Kir channels.
Surface Expression of Inward Rectifier Potassium
Channels Is Controlled by Selective Golgi Export*
and
*
The study was supported by Deutsche
Forschungsgemeinschaft Grant SFB 430-A2 (to N. K.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Present address: Universitäts-Kinderklinik, Abt. für
Hämatologie, Onkologie, und Immunologie, Im Neuenheimer Feld 156, 69120 Heidelberg, Germany. E-mail:
Clemens.Stockklausner@med.uni-heidelberg.de.
§
To whom correspondence should be addressed. Tel.:
0761-203-5141; Fax: 0761-203-5191; E-mail:
nikolaj.kloecker@physiologie.uni-freiburg.de.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
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