HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 19, 17150-17157, May 9, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/19/17150    most recent M301150200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Igbavboa, U. Articles by Wood, W. G. Search for Related Content PubMed PubMed Citation Articles by Igbavboa, U. Articles by Wood, W. G. Social Bookmarking                      What's this?

Cholesterol Distribution in the Golgi Complex of DITNC1 Astrocytes Is Differentially Altered by Fresh and Aged Amyloid -Peptide-(1-42)^*

Urule Igbavboa, Justine M. Pidcock, Leslie N. A. Johnson, Todd M. Malo, Ann E. Studniski, Su Yu^§, Grace Y. Sun^§, and W. Gibson Wood^¶

From the  Geriatric Research, Education and Clinical Center, Veterans Affairs Medical Center and the Department of Pharmacology, University of Minnesota School of Medicine, Minneapolis, Minnesota 55417 and the ^§ Department of Biochemistry, University of Missouri, Columbia, Missouri 65211

The Golgi complex plays an important role in cholesterol trafficking in cells, and amyloid -peptides (As) alter cholesterol trafficking. The hypothesis was tested that fresh and aged A-(1-42) would differentially modify Golgi cholesterol content in DINTC1 astrocytes and that the effects of A-(1-42) would be associated with the region of the Golgi complex. Two different methods were used to determine the effects of A-(1-42) on Golgi complex cholesterol. Confocal microscopy showed that fresh A-(1-42) significantly increased cholesterol and that aged A-(1-42) significantly reduced cholesterol content in the Golgi complex. Isolation of the Golgi complex into two fractions using density gradient centrifugation showed effects of aged A-(1-42) similar to those observed with confocal microscopy but revealed the novel finding that fresh A-(1-42) had opposite effects on the two Golgi fractions suggesting a specificity of A-(1-42) perturbation of the Golgi complex. Phosphatidylcholine-phospholipase D activity, cell membrane cholesterol, and apolipoprotein E levels were associated with effects of fresh A-(1-42) on cholesterol distribution but not with effects of aged A-(1-42), arguing against a common mechanism. Extracellular A-(1-42) targets the Golgi complex and disrupts cell cholesterol homeostasis, and this action of A-(1-42) could alter cell functions requiring optimal levels of cholesterol.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				X. Wang, Y. Wang, J. Zhang, H. P. Kim, S. W. Ryter, and A. M. K. Choi FLIP Protects against Hypoxia/Reoxygenation-Induced Endothelial Cell Apoptosis by Inhibiting Bax Activation Mol. Cell. Biol., June 1, 2005; 25(11): 4742 - 4751. [Abstract] [Full Text] [PDF]

				X. WANG, J. ZHANG, H. P. KIM, Y. WANG, A. M. K. CHOI, and S. W. RYTER Bcl-XL disrupts death-inducing signal complex formation in plasma membrane induced by hypoxia/reoxygenation FASEB J, December 1, 2004; 18(15): 1826 - 1833. [Abstract] [Full Text] [PDF]