HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 19, 17263-17268, May 9, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/19/17263    most recent M210486200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moore, M. L. Articles by McMillin, J. B. Search for Related Content PubMed PubMed Citation Articles by Moore, M. L. Articles by McMillin, J. B. Social Bookmarking                      What's this?

Upstream Stimulatory Factor Represses the Induction of Carnitine Palmitoyltransferase-I Expression by PGC-1^*

Meredith L. Moore, Edwards A. Park, and Jeanie B. McMillin^§

From the Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, UT-Houston Health Science Center and the Graduate School of Biomedical Sciences, The Texas Medical Center, Houston, Texas 77030 and the  Department of Pharmacology, The University of Tennessee, Memphis, Tennessee 38163

Transcriptional regulation of carnitine palmitoyltransferase-1 (CPT-1) is coordinated with contractile gene expression through cardiac-enriched transcription factors, GATA4 and SRF. Metabolic modulation of CPT-1 promoter activity has been described with the stimulation of gene expression by oleate that is mediated through the peroxisome proliferator-activated receptor (PPAR) pathway. The coactivator, peroxisomal proliferator-activated receptor  coactivator (PGC-1), enhances gene expression through interactions with nuclear hormone receptors and the myocyte enhancer factor 2 (MEF2) family. PGC-1 and MEF2A synergistically activate CPT-1 promoter activity. This stimulation is enhanced by mutation of the E-box sequences that flank the MEF2A binding site. These elements bind the upstream stimulatory factors (USF1 and USF2), which activate transcription in CV-1 fibroblasts. However, overexpression of the USF proteins in myocytes depresses CPT-1 activity and significantly reduces MEF2A and PGC-1 synergy. Co-immunoprecipitation studies demonstrate that PGC-1 and USF2 proteins can physically interact. Our studies demonstrate that PGC-1 stimulates CPT-1 gene expression through MEF2A. USF proteins have a novel role in repressing the expression of the CPT-1 gene and modulating the induction by the coactivator, PGC1.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				H. Wang, T. H. Peiris, A. Mowery, J. Le Lay, Y. Gao, and L. E. Greenbaum CCAAT/Enhancer Binding Protein-{beta} Is a Transcriptional Regulator of Peroxisome-Proliferator-Activated Receptor-{gamma} Coactivator-1{alpha} in the Regenerating Liver Mol. Endocrinol., July 1, 2008; 22(7): 1596 - 1605. [Abstract] [Full Text] [PDF]

				C. Handschin and B. M. Spiegelman Peroxisome Proliferator-Activated Receptor {gamma} Coactivator 1 Coactivators, Energy Homeostasis, and Metabolism Endocr. Rev., December 1, 2006; 27(7): 728 - 735. [Abstract] [Full Text] [PDF]

				B. Desvergne, L. Michalik, and W. Wahli Transcriptional Regulation of Metabolism Physiol Rev, April 1, 2006; 86(2): 465 - 514. [Abstract] [Full Text] [PDF]

				D. Phan, T. L. Rasmussen, O. Nakagawa, J. McAnally, P. D. Gottlieb, P. W. Tucker, J. A. Richardson, R. Bassel-Duby, and E. N. Olson BOP, a regulator of right ventricular heart development, is a direct transcriptional target of MEF2C in the developing heart Development, June 1, 2005; 132(11): 2669 - 2678. [Abstract] [Full Text] [PDF]

				Y. Okada, E. Matsuura, Z. Tozuka, R. Nagai, A. Watanabe, K. Matsumoto, K. Yasui, R. W. Jackman, T. Nakano, and T. Doi Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes Blood, October 1, 2004; 104(7): 2027 - 2034. [Abstract] [Full Text] [PDF]

				A. Baldan, J. Relat, P. F. Marrero, and D. Haro Functional interaction between peroxisome proliferator-activated receptors-{alpha} and Mef-2C on human carnitine palmitoyltransferase 1{beta} (CPT1{beta}) gene activation Nucleic Acids Res., September 8, 2004; 32(16): 4742 - 4749. [Abstract] [Full Text] [PDF]

				K. Sayasith, N. Bouchard, M. Sawadogo, J. G. Lussier, and J. Sirois Molecular Characterization and Role of Bovine Upstream Stimulatory Factor 1 and 2 in the Regulation of the Prostaglandin G/H Synthase-2 Promoter in Granulosa Cells J. Biol. Chem., February 20, 2004; 279(8): 6327 - 6336. [Abstract] [Full Text] [PDF]