HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 19, 17314-17319, May 9, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/19/17314    most recent M212170200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Koo, I. C. Articles by Stephens, R. S. Search for Related Content PubMed PubMed Citation Articles by Koo, I. C. Articles by Stephens, R. S. Social Bookmarking                      What's this?

A Developmentally Regulated Two-component Signal Transduction System in Chlamydia^*

Ingrid Chou Koo and Richard S. Stephens^§¶

From the  Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720-7360 and the ^§ Francis I. Proctor Foundation, University of California, San Francisco, California 94143

Two-component systems allow bacteria to adapt to changing environmental conditions and may induce developmental changes necessary for survival. Chlamydia trachomatis alternates between two distinct developmental forms, each optimized for survival in a separate niche. Transcriptional regulation of development is not understood. The C. trachomatis genome sequence revealed a single pair of genes (ctcB-ctcC) predicted to encode proteins with sequence conservation to bacterial two-component systems. Sequence analysis revealed that the sensor kinase, CtcB, possessed an energy-sensing PAS domain and phosphorylation site. The response regulator, CtcC, had homology to ⁵⁴ activators, possessing conserved receiver and ATPase domains and phosphorylation site, but lacked the C-terminal DNA-binding domain. ctcB and ctcC were expressed late in the developmental cycle, and both proteins were detected in EB lysates. Recombinant CtcB and CtcC were purified from denatured Escherichia coli inclusion bodies and refolded. CtcC was found to aggregate as dimers and tetramers in solution. In vitro phosphorylation assays showed that CtcB autophosphorylated in the presence of Mg²⁺, Mn²⁺, and Fe²⁺ and transferred the phosphoryl group in the presence of CtcC. Collectively, these results show that CtcB and CtcC function as a two-component system and are likely responsible for transcriptional regulation by ⁵⁴ holoenzyme during late-stage chlamydial development.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				S. N. Joslin and D. R. Hendrixson Analysis of the Campylobacter jejuni FlgR Response Regulator Suggests Integration of Diverse Mechanisms To Activate an NtrC-Like Protein J. Bacteriol., April 1, 2008; 190(7): 2422 - 2433. [Abstract] [Full Text] [PDF]

				D. L. Johnson and J. B. Mahony Chlamydophila pneumoniae PknD Exhibits Dual Amino Acid Specificity and Phosphorylates Cpn0712, a Putative Type III Secretion YscD Homolog J. Bacteriol., November 1, 2007; 189(21): 7549 - 7555. [Abstract] [Full Text] [PDF]

				S. Goellner, E. Schubert, E. Liebler-Tenorio, H. Hotzel, H. P. Saluz, and K. Sachse Transcriptional Response Patterns of Chlamydophila psittaci in Different In Vitro Models of Persistent Infection. Infect. Immun., August 1, 2006; 74(8): 4801 - 4808. [Abstract] [Full Text] [PDF]

				I. C. Koo, D. Walthers, P. S. Hefty, L. J. Kenney, and R. S. Stephens ChxR is a transcriptional activator in Chlamydia PNAS, January 17, 2006; 103(3): 750 - 755. [Abstract] [Full Text] [PDF]

				Z.-G. Zhang, H.-L. Zhou, T. Chen, Y. Gong, W.-H. Cao, Y.-J. Wang, J.-S. Zhang, and S.-Y. Chen Evidence for Serine/Threonine and Histidine Kinase Activity in the Tobacco Ethylene Receptor Protein NTHK2 Plant Physiology, October 1, 2004; 136(2): 2971 - 2981. [Abstract] [Full Text] [PDF]

				P. Brahmachary, M. G. Dashti, J. W. Olson, and T. R. Hoover Helicobacter pylori FlgR Is an Enhancer-Independent Activator of {sigma}54-RNA Polymerase Holoenzyme J. Bacteriol., July 15, 2004; 186(14): 4535 - 4542. [Abstract] [Full Text] [PDF]

				H. Xu, B. Gu, B. T. Nixon, and T. R. Hoover Purification and Characterization of the AAA+ Domain of Sinorhizobium meliloti DctD, a {sigma}54-Dependent Transcriptional Activator J. Bacteriol., June 1, 2004; 186(11): 3499 - 3507. [Abstract] [Full Text] [PDF]