Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M212227200 on March 5, 2003

J. Biol. Chem., Vol. 278, Issue 19, 17320-17327, May 9, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/19/17320    most recent
M212227200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Taouil, K.
Right arrow Articles by Puchelle, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taouil, K.
Right arrow Articles by Puchelle, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Stimulation of beta 2-Adrenergic Receptor Increases Cystic Fibrosis Transmembrane Conductance Regulator Expression in Human Airway Epithelial Cells through a cAMP/Protein Kinase A-independent Pathway*

Karima TaouilDagger , Jocelyne HinnraskyDagger , Coralie HologneDagger , Pascal Corlieu§, Jean-Michel Klossek, and Edith PuchelleDagger ||

From the Dagger  INSERM 514, IFR 53, Centre Hospitalier Universitaire Maison Blanche, Reims, 51092 Cedex, France, § Hopital Tenon, 4 rue de la Chine, 75020 Paris, France, and  Hopital Jean Bernard, BP 577, 86021 Poitiers, France

PSD-95/Dlg-A/ZO-1 (PDZ) domains play an essential role in determining cell polarity. The Na+/H+ exchanger regulatory factor (NHERF), also known as EBP50, contains two PDZ domains that mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. Moreover, it has been shown that cystic fibrosis transmembrane conductance regulator (CFTR) and beta 2-adrenergic receptor (beta 2AR) bind equally well to the PDZ1 domain of EBP50. We hypothesized that beta 2AR activation may regulate CFTR protein expression. To verify this, we evaluated the effects of a pharmacologically relevant concentration of salmeterol (2.10-7 M), a long acting beta 2AR agonist, on CFTR expression in primary human airway epithelial cells (HAEC). beta 2AR stimulation induced a time-dependent increase in apical CFTR protein expression, with a maximal response reached after treatment for 24 h. This effect was post-transcriptional, dependent upon the beta 2AR agonist binding to beta 2AR and independent of the known beta 2AR agonist-mediated cAMP/PKA pathway. We demonstrated by immunohistochemistry that CFTR, beta 2AR, and EBP50 localize to the apical membrane of HAEC. Analyses of anti-EBP50 protein immunoprecipitate showed that salmeterol induced an increase in the amount of CFTR that binds to EBP50. These data suggest that beta 2AR activation regulates the association of CFTR with EBP50 in polarized HAEC.

* This work was supported by INSERM, GlaxoSmithKline, and by the Association Vaincre la Mucoviscidose.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: INSERM 514, Centre Hospitalier Universitaire Maison Blanche 45, rue Cognacq Jay, 51092 Reims Cedex, France. Tel.: 33-3-26-78-77-70; Fax: 33-3-26-06-58-01; E-mail: epuche@worldnet.fr.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. F. Collawn, Z. Bebok, and S. Matalon
Search and Rescue: Finding Ways to Correct {Delta}F508 CFTR
Am. J. Respir. Cell Mol. Biol., April 1, 2009; 40(4): 385 - 387.
[Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
F. Delavoie, M. Molinari, M. Milliot, J.-M. Zahm, C. Coraux, J. Michel, and G. Balossier
Salmeterol Restores Secretory Functions in Cystic Fibrosis Airway Submucosal Gland Serous Cells
Am. J. Respir. Cell Mol. Biol., April 1, 2009; 40(4): 388 - 397.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
F. Bossard, A. Robay, G. Toumaniantz, S. Dahimene, F. Becq, J. Merot, and C. Gauthier
NHE-RF1 protein rescues {Delta}F508-CFTR function
Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1085 - L1094.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. H. Lee, W. Richter, W. Namkung, K. H. Kim, E. Kim, M. Conti, and M. G. Lee
Dynamic Regulation of Cystic Fibrosis Transmembrane Conductance Regulator by Competitive Interactions of Molecular Adaptors
J. Biol. Chem., April 6, 2007; 282(14): 10414 - 10422.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
E. Puchelle, J.-M. Zahm, J.-M. Tournier, and C. Coraux
Airway Epithelial Repair, Regeneration, and Remodeling after Injury in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2006; 3(8): 726 - 733.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Fragaki, C. Kileztky, C. Trentesaux, J.-M. Zahm, O. Bajolet, M. Johnson, and E. Puchelle
Downregulation by a long-acting beta2-adrenergic receptor agonist and corticosteroid of Staphylococcus aureus-induced airway epithelial inflammatory mediator production
Am J Physiol Lung Cell Mol Physiol, July 1, 2006; 291(1): L11 - L18.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Guerra, T. Fanelli, M. Favia, S. M. Riccardi, G. Busco, R. A. Cardone, S. Carrabino, E. J. Weinman, S. J. Reshkin, M. Conese, et al.
Na+/H+ Exchanger Regulatory Factor Isoform 1 Overexpression Modulates Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Activity in Human Airway 16HBE14o- Cells and Rescues {Delta}F508 CFTR Functional Expression in Cystic Fibrosis Cells
J. Biol. Chem., December 9, 2005; 280(49): 40925 - 40933.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Coraux, C. Kileztky, M. Polette, J. Hinnrasky, J.-M. Zahm, P. Devillier, S. de Bentzmann, and E. Puchelle
Airway Epithelial Integrity Is Protected by a Long-Acting {beta}2-Adrenergic Receptor Agonist
Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 605 - 612.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Huang, Y. You, M. S. Spoor, E. J. Richer, Vrinda. V. Kudva, R. C. Paige, M. P. Seiler, J. M. Liebler, J. Zabner, C. G. Plopper, et al.
Foxj1 is required for apical localization of ezrin in airway epithelial cells
J. Cell Sci., December 15, 2003; 116(24): 4935 - 4945.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement