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J. Biol. Chem., Vol. 278, Issue 2, 1165-1173, January 10, 2003
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§,
,
,
,
From the Retinoids, such as all-trans-retinoic
acid, play an essential role in the regulation of airway epithelial
cell growth, differentiation, and gene expression. Using cDNA
microarray, we identified a clone, DD4, that contains the cDNA of a
novel gene, spurt (secretory protein in upper respiratory
tracts) that was significantly induced by
all-trans-retinoic acid in primary cultured human
tracheobroncheal epithelia. Two alternatively spliced spurt
transcripts of 1090 and 1035 base pairs exist that contain the same
open reading frame expressing a 256-amino acid peptide. The full-length
spurt cDNA sequence spans a genomic DNA fragment of
7,313 bp, and the gene is located on chromosome 20q11.21.
spurt mRNA is notably expressed at high levels in human
nasal, tracheal, and lung tissues. In situ hybridization
demonstrated that spurt message is often present in
secretory cell types. The human spurt gene product is a
secretory protein that contains a distinct signal peptide sequence in
its first 19 amino acids. Mono-specific antibodies were generated to
characterize spurt expression. Our data demonstrate that
spurt is secreted onto the apical side of primary human
airway epithelial cultures and is present in clinical sputum samples.
spurt gene expression is higher in sputum and tissue
samples obtained from patients with chronic obstructive lung disease.
Our results provide the cloning and characterization of this
tissue-specific novel gene and its possible relationship with airway diseases.
Center for Comparative Respiratory Biology
and Medicine, Division of Pulmonary & Critical Care Medicine,
School of Medicine and the ¶ Department of Pathology, Medical
Center of the University of California, Davis, California 95616
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF417257, AF417256, and AF421369.
§ To whom correspondence should be addressed: Dept. of Environmental and Occupational Health, University of Pittsburgh, 3343 Forbes Ave., Pittsburgh, PA 15260. Tel.: 412-383-2157; Fax: 412-383-2123; E-mail: peterdi@pitt.edu.This article has been cited by other articles:
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