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J. Biol. Chem., Vol. 278, Issue 2, 1201-1205, January 10, 2003
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From the Hepadnaviral reverse transcription occurs
in subviral capsids in which the core protein surrounds the reverse
transcriptase ("polymerase") and the pregenomic RNA. The pregenomic
RNA is the template for reverse transcription and also the bicistronic
mRNA for core and polymerase. The pregenomic RNA structure and the capsid stoichiometry imply that vastly more core would be translated than polymerase. Previously, we found that duck hepatitis B virus polymerase unexpectedly accumulates in the cytoplasm (Yao, E., Gong,
Y., Chen, N., and Tavis, J. E. (2000) J. Virol.
74, 8648-8657). The production mechanism and function of the excess
polymerase are unknown. Here, we determined the kinetics of expression
and degradation of polymerase and core in cells producing virus.
Polymerase was translated 10% as rapidly as core, the half-life of
nonencapsidated polymerase was very short, core had a very long
half-life, and very few polymerase molecules were encapsidated. The
presence of excess polymerase indicates that the translation rate of
the polymerase is not limiting for encapsidation. Therefore,
encapsidation must be regulated by other events, most likely binding of
the polymerase to the pregenomic RNA. These data support the hypothesis that polymerase may have functions beyond copying the viral genome by
demonstrating that the polymerase is a cytoplasmic protein that
is only rarely encapsidated.
Kinetics of Synthesis and Turnover of the Duck Hepatitis B Virus
Reverse Transcriptase*
and§¶
Department of Molecular
Microbiology and Immunology and from the § Saint
Louis University Liver Center, Saint Louis University School of
Medicine, St. Louis, Missouri 63104
*
This work was supported by Grants AI38447 and CA91327 from
the National Institutes of Health (to J. E. T.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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