Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M210266200 on November 4, 2002

J. Biol. Chem., Vol. 278, Issue 2, 1206-1211, January 10, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/2/1206    most recent
M210266200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baqui, M.
Right arrow Articles by Bianco, A. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baqui, M.
Right arrow Articles by Bianco, A. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Human Type 3 Iodothyronine Selenodeiodinase Is Located in the Plasma Membrane and Undergoes Rapid Internalization to Endosomes*

Munira Baqui, Diego Botero, Balazs Gereben, Cyntia Curcio, John W. Harney, Domenico Salvatore, Kenji Sorimachi, P. Reed Larsen, and Antonio C. BiancoDagger

From the Thyroid Section, Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115

The type 3 iodothyronine selenodeiodinase (D3) is an integral membrane protein that inactivates thyroid hormones. By using immunofluorescence cytochemistry confocal microscopy of live or fixed cells transiently expressing FLAG-tagged human D3 or monkey hepatocarcinoma cells expressing endogenous D3, we identified D3 in the plasma membrane. It co-localizes with Na,K-ATPase alpha , with the early endosomal marker EEA-1 and clathrin, but not with two endoplasmic reticulum resident proteins. Most of the D3 molecule is extracellular and can be biotinylated with a cell-impermeant probe. There is constant internalization of D3 that is blocked by sucrose or methyl-beta -cyclodextrin-containing medium. Exposing cells to a weak base such as primaquine increases the pool of internalized D3, suggesting that D3 is recycled between plasma membrane and early endosomes. Such recycling could account for the much longer half-life of D3 (12 h) than the thyroxine activating members of the selenodeiodinase family, type 1 (D1; 8 h) or type 2 (D2; 2 h) deiodinase. The extracellular location of D3 gives ready access to circulating thyroid hormones, explaining its capacity for rapid inactivation of circulating thyroxine and triiodothyronine in patients with hemangiomas and its blockade of the access of maternal thyroid hormones to the human fetus.

* This work was supported by Grants DK 36256 and DK 44128 from the National Institutes of Health.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: 77 Avenue Louis Pasteur, HIM Bldg., Rm. 560, Boston, MA 02115. Tel.: 617-525-5153; Fax: 617-731-4718; E-mail: abianco@partners.org.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
B. Gereben, A. M. Zavacki, S. Ribich, B. W. Kim, S. A. Huang, W. S. Simonides, A. Zeold, and A. C. Bianco
Cellular and Molecular Basis of Deiodinase-Regulated Thyroid Hormone Signaling
Endocr. Rev., December 1, 2008; 29(7): 898 - 938.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
G. D. V. Sagar, B. Gereben, I. Callebaut, J.-P. Mornon, A. Zeold, C. Curcio-Morelli, J. W. Harney, C. Luongo, M. A. Mulcahey, P. R. Larsen, et al.
The Thyroid Hormone-Inactivating Deiodinase Functions as a Homodimer
Mol. Endocrinol., June 1, 2008; 22(6): 1382 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. B. Anderson, A. L. Goldberg, and M. Whitman
Identification of a Novel Pool of Extracellular Pro-myostatin in Skeletal Muscle
J. Biol. Chem., March 14, 2008; 283(11): 7027 - 7035.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
E. C. H. Friesema, G. G. J. M. Kuiper, J. Jansen, T. J. Visser, and M. H. A. Kester
Thyroid Hormone Transport by the Human Monocarboxylate Transporter 8 and Its Rate-Limiting Role in Intracellular Metabolism
Mol. Endocrinol., November 1, 2006; 20(11): 2761 - 2772.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. Chu, R. Piper, S. Richardson, Y. Watanabe, P. Patel, and D. D. Heistad
Endocytosis of Extracellular Superoxide Dismutase Into Endothelial Cells: Role of the Heparin-Binding Domain
Arterioscler. Thromb. Vasc. Biol., September 1, 2006; 26(9): 1985 - 1990.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. A. Huang, M. A. Mulcahey, A. Crescenzi, M. Chung, B. W. Kim, C. Barnes, W. Kuijt, H. Turano, J. Harney, and P. R. Larsen
Transforming Growth Factor-{beta} Promotes Inactivation of Extracellular Thyroid Hormones via Transcriptional Stimulation of Type 3 Iodothyronine Deiodinase
Mol. Endocrinol., December 1, 2005; 19(12): 3126 - 3136.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
B. McKinnon, H. Li, K. Richard, and R. Mortimer
Synthesis of Thyroid Hormone Binding Proteins Transthyretin and Albumin by Human Trophoblast
J. Clin. Endocrinol. Metab., December 1, 2005; 90(12): 6714 - 6720.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
G. J Beckett and J. R Arthur
Selenium and endocrine systems
J. Endocrinol., March 1, 2005; 184(3): 455 - 465.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. H. A. Gouveia, M. A. Christoffolete, C. R. Zaitune, J. M. Dora, J. W. Harney, A. L. Maia, and A. C. Bianco
Type 2 Iodothyronine Selenodeiodinase Is Expressed throughout the Mouse Skeleton and in the MC3T3-E1 Mouse Osteoblastic Cell Line during Differentiation
Endocrinology, January 1, 2005; 146(1): 195 - 200.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
B. W. Kim, A. M. Zavacki, C. Curcio-Morelli, M. Dentice, J. W. Harney, P. R. Larsen, and A. C. Bianco
Endoplasmic Reticulum-Associated Degradation of the Human Type 2 Iodothyronine Deiodinase (D2) is Mediated via an Association between Mammalian UBC7 and the Carboxyl Region of D2
Mol. Endocrinol., December 1, 2003; 17(12): 2603 - 2612.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Callebaut, C. Curcio-Morelli, J.-P. Mornon, B. Gereben, C. Buettner, S. Huang, B. Castro, T. L. Fonseca, J. W. Harney, P. R. Larsen, et al.
The Iodothyronine Selenodeiodinases Are Thioredoxin-fold Family Proteins Containing a Glycoside Hydrolase Clan GH-A-like Structure
J. Biol. Chem., September 19, 2003; 278(38): 36887 - 36896.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. G. J. M. Kuiper, W. Klootwijk, and T. J. Visser
Substitution of Cysteine for Selenocysteine in the Catalytic Center of Type III Iodothyronine Deiodinase Reduces Catalytic Efficiency and Alters Substrate Preference
Endocrinology, June 1, 2003; 144(6): 2505 - 2513.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement