Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M207264200 on November 4, 2002

J. Biol. Chem., Vol. 278, Issue 2, 732-738, January 10, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/2/732    most recent
M207264200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shibusawa, N.
Right arrow Articles by Wondisford, F. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shibusawa, N.
Right arrow Articles by Wondisford, F. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Thyroid Hormone Receptor DNA Binding Is Required for Both Positive and Negative Gene Regulation*

Nobuyuki ShibusawaDagger , Anthony N. Hollenberg§, and Fredric E. WondisfordDagger

From the Dagger  Section of Endocrinology, Department of Medicine, the University of Chicago, Chicago, Illinois 60637 and § Division of Endocrinology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215

The beta  isoform of thyroid hormone receptor (TR-beta ) has a key role in the feedback regulation of the hypothalamic-pituitary-thyroid (H-P-T) axis. The mechanism of trans-repression of the hypothalamic thyrotropin-releasing hormone (TRH) and pituitary thyroid-stimulating hormone (TSH) subunit genes, however, remains poorly understood. A number of distinct mechanisms for TR-beta -mediated negative regulation by thyroid hormone have been proposed, including those that require and do not require DNA binding. To clarify the importance of DNA binding in negative regulation, we constructed a DNA-binding mutant of TR-beta in which two amino acids within the P box were altered (GSG for EGG) to resemble that found in the glucocorticoid receptor (GR). We termed this mutant GS125, and as expected, it displayed low binding affinities for positive and negative thyroid hormone-response element (pTRE and nTRE, respectively) in gel-mobility shift assays. In transient transfection assays, the GS125 mutant abolished transactivation on three classic pTREs (DR+4, LAP, and PAL) and all negatively regulated promoters in the H-P-T axis (TRH, TSH-beta , and TSH-alpha ). However, GS125 TR-beta bound to a composite TR/GR-response element and was fully functional on this hybrid TR/GR-response element. Moreover, the GS125 TR-beta mutant displayed normal interactions with transcriptional cofactors in mammalian two-hybrid assays. These data do not support a DNA-binding independent mechanism for thyroid hormone negative regulation in the H-P-T axis.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Section of Endocrinology, Dept. of Medicine, the University of Chicago, 5841 S. Maryland Ave., MC 1027, Chicago, IL 60637. Tel.: 773-702-6217; Fax: 773-834-0486.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. S. Machado, A. Sabet, L. A. Santiago, A. R. Sidhaye, M. I. Chiamolera, T. M. Ortiga-Carvalho, and F. E. Wondisford
From the Cover: A thyroid hormone receptor mutation that dissociates thyroid hormone regulation of gene expression in vivo
PNAS, June 9, 2009; 106(23): 9441 - 9446.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. I. Chiamolera and F. E. Wondisford
Thyrotropin-Releasing Hormone and the Thyroid Hormone Feedback Mechanism
Endocrinology, March 1, 2009; 150(3): 1091 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
A. Wulf, M. G Wetzel, M. Kebenko, M. Kroger, A. Harneit, J. Merz, and J. M Weitzel
The role of thyroid hormone receptor DNA binding in negative thyroid hormone-mediated gene transcription
J. Mol. Endocrinol., July 1, 2008; 41(1): 25 - 34.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Dong, C. L. Yauk, A. Williams, A. Lee, G. R. Douglas, and M. G. Wade
Hepatic Gene Expression Changes in Hypothyroid Juvenile Mice: Characterization of a Novel Negative Thyroid-Responsive Element
Endocrinology, August 1, 2007; 148(8): 3932 - 3940.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
X. Zhu, A. S. Gleiberman, and M. G. Rosenfeld
Molecular Physiology of Pituitary Development: Signaling and Transcriptional Networks
Physiol Rev, July 1, 2007; 87(3): 933 - 963.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. F. R. Velasco, M. Togashi, P. G. Walfish, R. P. Pessanha, F. N. Moura, G. B. Barra, P. Nguyen, R. Rebong, C. Yuan, L. A. Simeoni, et al.
Thyroid Hormone Response Element Organization Dictates the Composition of Active Receptor
J. Biol. Chem., April 27, 2007; 282(17): 12458 - 12466.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Matsushita, S. Sasaki, Y. Kashiwabara, K. Nagayama, K. Ohba, H. Iwaki, H. Misawa, K. Ishizuka, and H. Nakamura
Essential Role of GATA2 in the Negative Regulation of Thyrotropin {beta} Gene by Thyroid Hormone and Its Receptors
Mol. Endocrinol., April 1, 2007; 21(4): 865 - 884.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
F. Flamant, K. Gauthier, and J. Samarut
Thyroid Hormones Signaling Is Getting More Complex: STORMs Are Coming
Mol. Endocrinol., February 1, 2007; 21(2): 321 - 333.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
G. Calamita, M. Moreno, D. Ferri, E. Silvestri, P. Roberti, L. Schiavo, P. Gena, M. Svelto, and F. Goglia
Triiodothyronine modulates the expression of aquaporin-8 in rat liver mitochondria
J. Endocrinol., January 1, 2007; 192(1): 111 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
F. Flamant, J. D. Baxter, D. Forrest, S. Refetoff, H. Samuels, T. S. Scanlan, B. Vennstrom, and J. Samarut
International Union of Pharmacology. LIX. The Pharmacology and Classification of the Nuclear Receptor Superfamily: Thyroid Hormone Receptors
Pharmacol. Rev., December 1, 2006; 58(4): 705 - 711.
[Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Hashimoto, M. Yamada, S. Matsumoto, T. Monden, T. Satoh, and M. Mori
Mouse Sterol Response Element Binding Protein-1c Gene Expression Is Negatively Regulated by Thyroid Hormone
Endocrinology, September 1, 2006; 147(9): 4292 - 4302.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. M. Santos, V. Afonso, G. B. Barra, M. Togashi, P. Webb, F. A. R. Neves, N. Lomri, and A. Lomri
Negative Regulation of Superoxide Dismutase-1 Promoter by Thyroid Hormone
Mol. Pharmacol., September 1, 2006; 70(3): 793 - 800.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
M. Nygard, N. Becker, B. Demeneix, K. Pettersson, and M. Bondesson
Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.
J. Mol. Endocrinol., June 1, 2006; 36(3): 517 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. M. Storey, S. Gentile, H. Ullah, A. Russo, M. Muessel, C. Erxleben, and D. L. Armstrong
Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone
PNAS, March 28, 2006; 103(13): 5197 - 5201.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Meng, P. Webb, Y.-F. Yang, M. Shuen, A. F. Yousef, J. D. Baxter, J. S. Mymryk, and P. G. Walfish
E1A and a nuclear receptor corepressor splice variant (N-CoRI) are thyroid hormone receptor coactivators that bind in the corepressor mode
PNAS, May 3, 2005; 102(18): 6267 - 6272.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Temple, R. N. Cohen, S. R. Wondisford, C. Yu, D. Deplewski, and F. E. Wondisford
An Intact DNA-binding Domain Is Not Required for Peroxisome Proliferator-activated Receptor {gamma} (PPAR{gamma}) Binding and Activation on Some PPAR Response Elements
J. Biol. Chem., February 4, 2005; 280(5): 3529 - 3540.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
X. Shen, Q.-L. Li, G. A. Brent, and T. C. Friedman
Regulation of regional expression in rat brain PC2 by thyroid hormone/characterization of novel negative thyroid hormone response elements in the PC2 promoter
Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E236 - E245.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S.-W. Kim, S.-J. Hong, K. M. Kim, S.-C. Ho, E. C. So, J. W. Harney, and P. R. Larsen
A Novel Cell Type-Specific Mechanism for Thyroid Hormone-Dependent Negative Regulation of the Human Type 1 Deiodinase Gene
Mol. Endocrinol., December 1, 2004; 18(12): 2924 - 2936.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Ishii, M. Yamada, T. Satoh, T. Monden, K. Hashimoto, N. Shibusawa, K. Onigata, A. Morikawa, and M. Mori
Aberrant Dynamics of Histone Deacetylation at the Thyrotropin-Releasing Hormone Gene in Resistance to Thyroid Hormone
Mol. Endocrinol., July 1, 2004; 18(7): 1708 - 1720.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
F. Guo, K. Bakal, Y. Minokoshi, and A. N. Hollenberg
Leptin Signaling Targets the Thyrotropin-Releasing Hormone Gene Promoter in Vivo
Endocrinology, May 1, 2004; 145(5): 2221 - 2227.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Villa, J. Santiago, B. Belandia, and A. Pascual
A Response Unit in the First Exon of the {beta}-Amyloid Precursor Protein Gene Containing Thyroid Hormone Receptor and Sp1 Binding Sites Mediates Negative Regulation by 3,5,3'-Triiodothyronine
Mol. Endocrinol., April 1, 2004; 18(4): 863 - 873.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Ishizuka and M. A. Lazar
The N-CoR/Histone Deacetylase 3 Complex Is Required for Repression by Thyroid Hormone Receptor
Mol. Cell. Biol., August 1, 2003; 23(15): 5122 - 5131.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement