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J. Biol. Chem., Vol. 278, Issue 20, 17655-17663, May 16, 2003
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From the Nucleosomes inhibit DNA repair in
vitro, suggesting that chromatin remodeling activities might be
required for efficient repair in vivo. To investigate how
structural and dynamic properties of nucleosomes affect damage
recognition and processing, we investigated repair of UV lesions by
photolyase on a nucleosome positioned at one end of a 226-bp-long DNA
fragment. Repair was slow in the nucleosome but efficient outside. No
disruption or movement of the nucleosome was observed after UV
irradiation and during repair. However, incubation with the nucleosome
remodeling complex SWI/SNF and ATP altered the conformation of
nucleosomal DNA as judged by UV photo-footprinting and promoted more
homogeneous repair. Incubation with yISW2 and ATP moved the
nucleosome to a more central position, thereby altering the repair
pattern. This is the first demonstration that two different chromatin
remodeling complexes can act on UV-damaged nucleosomes and modulate
repair. Similar activities might relieve the inhibitory effect of
nucleosomes on DNA repair processes in living cells.
Chromatin Remodeling Activities Act on UV-damaged Nucleosomes and
Modulate DNA Damage Accessibility to Photolyase*
§,
,,**
Institut für Zellbiologie and
¶ Institut für Molekularbiologie, Departement Biologie,
ETH-Hönggerberg, CH-8093 Zürich, Switzerland and
Program in Molecular Medicine, University of Massachusetts
Medical School, Worcester, Massachusetts 01605
*
This work was supported by grants from the Swiss National
Science Foundation, the ETH Zürich, and the Roche Research
Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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