JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M301676200 on March 11, 2003

J. Biol. Chem., Vol. 278, Issue 20, 17688-17700, May 16, 2003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
278/20/17688    most recent
M301676200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tan, L.
Right arrow Articles by Goldring, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tan, L.
Right arrow Articles by Goldring, M. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Egr-1 Mediates Transcriptional Repression of COL2A1 Promoter Activity by Interleukin-1beta *

Lujian TanDagger , Haibing PengDagger , Makoto OsakiDagger , Bob K. ChoyDagger , Philip E. AuronDagger , Linda J. Sandell§, and Mary B. GoldringDagger

From the Dagger  Rheumatology Division, Beth Israel Deaconess Medical Center and New England Baptist Bone & Joint Institute, Harvard Institutes of Medicine, Boston, Massachusetts 02115 and the § Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri 63110

Following induction and activation of the early growth response (Egr)-1 transcription factor in human chondrocytes, interleukin-1beta (IL-1beta ) suppresses the expression of the type II collagen gene (COL2A1), associated with induction of Egr-1 binding activity in nuclear extracts. The COL2A1 proximal promoter contains overlapping binding sites for Egr-1 and Sp1 family members at -119/-112 bp and -81/-74 bp. Mutations that block binding of Sp1 and Sp3 to either site markedly reduce constitutive expression of the core promoter. IL-1beta -induced Egr-1 binds strongly to the -119/-112 bp site, and mutations that block Egr-1 binding prevent inhibition by IL-1beta . Cotransfection with pCMV-Egr1 potentiates the inhibition of COL2A1 promoter activity by IL-1beta , whereas overexpression of dominant-negative Egr-1 mutant, Wilm's tumor-1 (WT1)/Egr1, Sp1, or Sp3 reverses the inhibition by IL-1beta . Cotransfection of pGL2-COL2/Gal4, in which we substituted the critical residue for Egr-1 binding with a Gal4 binding domain and a pCMV-Gal4-Egr1 chimera permits an inhibitory response to IL-1beta that is reversed by overexpression of Gal4-CBP. Our results indicate that IL-1beta -induced activation of Egr-1 binding is required for inhibition of COL2A1 proximal promoter activity and suggest that Egr-1 acts as a repressor of a constitutively expressed collagen gene by preventing interactions between Sp1 and the general transcriptional machinery.

* This work was supported in part by National Institutes of Health Grants AR45378 and AG22021 (to M. B. G.) and CA68544 (to P. E. A.) and a Biomedical Science Grant from the Arthritis Foundation (to M. B. G.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Harvard Institutes of Medicine, Room 246, New England Baptist Bone & Joint Institute, 4 Blackfan Circle, Boston, MA 02115. Tel.: 617-667-0742; Fax: 617-975-5299; E-mail: mgoldrin@bidmc.harvard.edu.

Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. B. Fitzgerald, M. Jin, D. H. Chai, P. Siparsky, P. Fanning, and A. J. Grodzinsky
Shear- and Compression-induced Chondrocyte Transcription Requires MAPK Activation in Cartilage Explants
J. Biol. Chem., March 14, 2008; 283(11): 6735 - 6743.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Poree, M. Kypriotou, C. Chadjichristos, G. Beauchef, E. Renard, F. Legendre, M. Melin, S. Gueret, D.-J. Hartmann, F. Mallein-Gerin, et al.
Interleukin-6 (IL-6) and/or Soluble IL-6 Receptor Down-regulation of Human Type II Collagen Gene Expression in Articular Chondrocytes Requires a Decrease of Sp1{middle dot}Sp3 Ratio and of the Binding Activity of Both Factors to the COL2A1 Promoter
J. Biol. Chem., February 22, 2008; 283(8): 4850 - 4865.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
S. R. Davies, L.-W. Chang, D. Patra, X. Xing, K. Posey, J. Hecht, G. D. Stormo, and L. J. Sandell
Computational identification and functional validation of regulatory motifs in cartilage-expressed genes
Genome Res., October 1, 2007; 17(10): 1438 - 1447.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. R. Hill, A. Papafili, H. Booth, P. Lawson, M. Hubner, H. Beynon, C. Read, G. Lindahl, R. P. Marshall, R. J. McAnulty, et al.
Functional Prostaglandin-Endoperoxide Synthase 2 Polymorphism Predicts Poor Outcome in Sarcoidosis
Am. J. Respir. Crit. Care Med., October 15, 2006; 174(8): 915 - 922.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-J. Chen, H. Ning, W. Ishida, S. Sodin-Semrl, S. Takagawa, Y. Mori, and J. Varga
The Early-Immediate Gene EGR-1 Is Induced by Transforming Growth Factor-beta and Mediates Stimulation of Collagen Gene Expression
J. Biol. Chem., July 28, 2006; 281(30): 21183 - 21197.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. B. James, A.-M. Conway, and B. J. Morris
Regulation of the Neuronal Proteasome by Zif268 (Egr1)
J. Neurosci., February 1, 2006; 26(5): 1624 - 1634.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Ijiri, L. F. Zerbini, H. Peng, R. G. Correa, B. Lu, N. Walsh, Y. Zhao, N. Taniguchi, X.-L. Huang, H. Otu, et al.
A Novel Role for GADD45{beta} as a Mediator of MMP-13 Gene Expression during Chondrocyte Terminal Differentiation
J. Biol. Chem., November 18, 2005; 280(46): 38544 - 38555.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
Y. Moon, W. C. Glasgow, and T. E. Eling
Curcumin Suppresses Interleukin 1{beta}-Mediated Microsomal Prostaglandin E Synthase 1 by Altering Early Growth Response Gene 1 and Other Signaling Pathways
J. Pharmacol. Exp. Ther., November 1, 2005; 315(2): 788 - 795.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Furumatsu, M. Tsuda, K. Yoshida, N. Taniguchi, T. Ito, M. Hashimoto, T. Ito, and H. Asahara
Sox9 and p300 Cooperatively Regulate Chromatin-mediated Transcription
J. Biol. Chem., October 21, 2005; 280(42): 35203 - 35208.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Zhang, X. Liu, J. G. Cogan, M. D. Fuerst, J. A. Polikandriotis, R. J. Kelm Jr., and A. R. Strauch
YB-1 Coordinates Vascular Smooth Muscle {alpha}-Actin Gene Activation by Transforming Growth Factor {beta}1 and Thrombin during Differentiation of Human Pulmonary Myofibroblasts
Mol. Biol. Cell, October 1, 2005; 16(10): 4931 - 4940.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Imamura, C. Imamura, Y. Iwamoto, and L. J. Sandell
Transcriptional Co-activators CREB-binding Protein/p300 Increase Chondrocyte Cd-rap Gene Expression by Multiple Mechanisms Including Sequestration of the Repressor CCAAT/Enhancer-binding Protein
J. Biol. Chem., April 29, 2005; 280(17): 16625 - 16634.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Qiao, S. R. Padilla, and P. D. Benya
Transforming Growth Factor (TGF)-{beta}-activated Kinase 1 Mimics and Mediates TGF-{beta}-induced Stimulation of Type II Collagen Synthesis in Chondrocytes Independent of Col2a1 Transcription and Smad3 Signaling
J. Biol. Chem., April 29, 2005; 280(17): 17562 - 17571.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Furumatsu, M. Tsuda, N. Taniguchi, Y. Tajima, and H. Asahara
Smad3 Induces Chondrogenesis through the Activation of SOX9 via CREB-binding Protein/p300 Recruitment
J. Biol. Chem., March 4, 2005; 280(9): 8343 - 8350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Mostecki, B. M. Showalter, and P. B. Rothman
Early Growth Response-1 Regulates Lipopolysaccharide-induced Suppressor of Cytokine Signaling-1 Transcription
J. Biol. Chem., January 28, 2005; 280(4): 2596 - 2605.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
B. Grigolo, L. Roseti, L. De Franceschi, A. Piacentini, L. Cattini, M. Manfredini, R. Faccini, and A. Facchini
Molecular and Immunohistological Characterization of Human Cartilage Two Years Following Autologous Cell Transplantation
J. Bone Joint Surg. Am., January 1, 2005; 87(1): 46 - 57.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
R. S. Peterson, R. A. Andhare, K. T. Rousche, W. Knudson, W. Wang, J. B. Grossfield, R. O. Thomas, R. E. Hollingsworth, and C. B. Knudson
CD44 modulates Smad1 activation in the BMP-7 signaling pathway
J. Cell Biol., September 27, 2004; 166(7): 1081 - 1091.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yokota, M. B. Goldring, and H. B. Sun
CITED2-mediated Regulation of MMP-1 and MMP-13 in Human Chondrocytes under Flow Shear
J. Biol. Chem., November 21, 2003; 278(47): 47275 - 47280.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Chadjichristos, C. Ghayor, M. Kypriotou, G. Martin, E. Renard, L. Ala-Kokko, G. Suske, B. de Crombrugghe, J.-P. Pujol, and P. Galera
Sp1 and Sp3 Transcription Factors Mediate Interleukin-1{beta} Down-regulation of Human Type II Collagen Gene Expression in Articular Chondrocytes
J. Biol. Chem., October 10, 2003; 278(41): 39762 - 39772.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.