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J. Biol. Chem., Vol. 278, Issue 20, 17710-17715, May 16, 2003

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Two Subtypes of Ecdysis-triggering Hormone Receptor in Drosophila melanogaster^*

Yoonseong Park^§¶, Young-Joon Kim, Vincent Dupriez, and Michael E. Adams^§**

From the Departments of  Entomology and ^§ Cell Biology and Neuroscience, University of California, Riverside, California 92521 and  Euroscreen s.a., Rue Adrienne Bolland 47, B-6041 Gosselies, Belgium

Insect ecdysis is a hormonally programmed physiological sequence that enables insects to escape their old cuticle at the end of each developmental stage. The immediate events leading to ecdysis, which are initiated upon release of ecdysis-triggering hormones (ETH) into the bloodstream, include respiratory inflation and sequential stereotypic behaviors that facilitate shedding of the cuticle. Here we report that the Drosophila gene CG5911 encodes two functionally distinct subtypes of G protein-coupled receptors through alternative splicing (CG5911a and CG5911b) that respond preferentially to ecdysis-triggering hormones of flies and moths. These subtypes show differences in ligand sensitivity and specificity, suggesting that they may play separate roles in ETH signaling. At significantly higher concentrations (>100-fold), certain insect and vertebrate peptides also activate these receptors, providing evidence that CG5911 is evolutionarily related to the thyrotropin-releasing hormone and neuromedin U receptors. The ETH signaling system in insects is a vital system that provides opportunities for the construction of models for the molecular basis of stereotypic animal behavior as well as a target for the design of more sophisticated insect-selective pest control strategies.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EBI Data Bank with accession number(s) AY220741 and AY220742.

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