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Originally published In Press as doi:10.1074/jbc.M301119200 on February 13, 2003
J. Biol. Chem., Vol. 278, Issue 20, 17710-17715, May 16, 2003
Two Subtypes of Ecdysis-triggering Hormone Receptor in
Drosophila melanogaster*
Yoonseong
Park §¶,
Young-Joon
Kim ,
Vincent
Dupriez , and
Michael E.
Adams §**
From the Departments of Entomology and
§ Cell Biology and Neuroscience, University of
California, Riverside, California 92521 and Euroscreen s.a.,
Rue Adrienne Bolland 47, B-6041 Gosselies, Belgium
Insect ecdysis is a hormonally programmed
physiological sequence that enables insects to escape their old cuticle
at the end of each developmental stage. The immediate events leading to
ecdysis, which are initiated upon release of ecdysis-triggering
hormones (ETH) into the bloodstream, include respiratory inflation and sequential stereotypic behaviors that facilitate shedding of the cuticle. Here we report that the Drosophila gene
CG5911 encodes two functionally distinct subtypes of G
protein-coupled receptors through alternative splicing (CG5911a and
CG5911b) that respond preferentially to ecdysis-triggering hormones of
flies and moths. These subtypes show differences in ligand sensitivity
and specificity, suggesting that they may play separate roles in ETH
signaling. At significantly higher concentrations (>100-fold), certain
insect and vertebrate peptides also activate these receptors, providing evidence that CG5911 is evolutionarily related to the
thyrotropin-releasing hormone and neuromedin U receptors. The
ETH signaling system in insects is a vital system that provides
opportunities for the construction of models for the molecular basis of
stereotypic animal behavior as well as a target for the design of more
sophisticated insect-selective pest control strategies.
*
This work was supported by National Institutes of Health
Grant AI-40555.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AY220741 and AY220742.
¶
Present address: Dept. of Entomology, 123 West Waters Hall,
Kansas State University, Manhattan, KS 66506. E-mail:
ypark@oznet.ksu.edu.
**
To whom correspondence should be addressed: Dept. of Cell Biology
and Neuroscience, 5429 Boyce Hall, University of California, Riverside, CA 92521. Tel.: 909-787-4746; Fax: 909-787-3087; E-mail: adams@mail.ucr.edu.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

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