|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 278, Issue 20, 17852-17858, May 16, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Covalent attachment of heme to apocytochromes
c in bacteria occurs on the outside of the cytoplasmic
membrane and requires two reduced cysteinyls at the heme binding site.
A constructed ResA-deficient Bacillus subtilis strain was
found to lack c-type cytochromes. Cytochrome c
synthesis was restored in the mutant by: (i) in trans
expression of resA; (ii) deficiency in BdbD, a
thiol-disulfide oxidoreductase that catalyzes formation of an intramolecular disulfide bond in apocytochrome c after
transfer of the polypeptide across the cytoplasmic membrane; or (iii)
by addition of the reductant dithiothreitol to the growth
medium. In vivo studies of ResA showed that it is
membrane-associated with its thioredoxin-like domain on the outside of
the cytoplasmic membrane. Analysis of a soluble form of the protein
revealed two redox reactive cysteine residues with a midpoint potential
of about
Bacillus subtilis ResA Is a Thiol-Disulfide
Oxidoreductase involved in Cytochrome c Synthesis*
§,, and
Department of Cell and Organism Biology,
Lund University, Sölvegatan 35, SE-22362 Lund, Sweden and the
¶ Centre for Metalloprotein Spectroscopy and Biology, School of
Chemical Sciences and Pharmacy, University of East Anglia,
Norwich NR4 7TJ, United Kingdom
340 mV at pH 7. We conclude that ResA, probably together with another thiol-disulfide oxidoreductase, CcdA, is required for the
reduction of the cysteinyls in the heme binding site of apocytochrome
c.
*
This work was supported by a travel grant from The Swedish
Royal Academy of Sciences and grants from the Swedish Research Council
(contract 621-2001-3125) (to L. H.), the Biotechnology and Biological
Sciences Research Council of the United Kingdom, The Wellcome
Trust, and The Royal Society (to N. L. B.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: School of Chemical
Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK.
Tel.: 01603-592003; Fax: 01603-592003; E-mail:
n.le-brun@uea.ac.uk.
Copyright © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Crow, Y. Liu, M. C. Moller, N. E. Le Brun, and L. Hederstedt Structure and Functional Properties of Bacillus subtilis Endospore Biogenesis Factor StoA J. Biol. Chem., April 10, 2009; 284(15): 10056 - 10066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sanders, S. Turkarslan, D.-W. Lee, O. Onder, R. G. Kranz, and F. Daldal The Cytochrome c Maturation Components CcmF, CcmH, and CcmI Form a Membrane-integral Multisubunit Heme Ligation Complex J. Biol. Chem., October 31, 2008; 283(44): 29715 - 29722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. C. Hodson, A. Lewin, L. Hederstedt, and N. E. Le Brun The Active-Site Cysteinyls and Hydrophobic Cavity Residues of ResA Are Important for Cytochrome c Maturation in Bacillus subtilis J. Bacteriol., July 1, 2008; 190(13): 4697 - 4705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Moller and L. Hederstedt Extracytoplasmic Processes Impaired by Inactivation of trxA (Thioredoxin Gene) in Bacillus subtilis J. Bacteriol., July 1, 2008; 190(13): 4660 - 4665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Geng, Y. Zhu, K. Mullen, C. S. Zuber, and M. M. Nakano Characterization of ResDE-Dependent fnr Transcription in Bacillus subtilis J. Bacteriol., March 1, 2007; 189(5): 1745 - 1755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sanders, C. Boulay, and F. Daldal Membrane-Spanning and Periplasmic Segments of CcmI Have Distinct Functions during Cytochrome c Biogenesis in Rhodobacter capsulatus J. Bacteriol., February 1, 2007; 189(3): 789 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lewin, A. Crow, A. Oubrie, and N. E. Le Brun Molecular Basis for Specificity of the Extracytoplasmic Thioredoxin ResA J. Biol. Chem., November 17, 2006; 281(46): 35467 - 35477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, Y. Hu, X. Guo, E. Lescop, Y. Li, B. Xia, and C. Jin The Bacillus subtilis YkuV Is a Thiol:Disulfide Oxidoreductase Revealed by Its Redox Structures and Activity J. Biol. Chem., March 24, 2006; 281(12): 8296 - 8304. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Colbert, Q. Wu, P. J. A. Erbel, K. H. Gardner, and J. Deisenhofer Mechanism of substrate specificity in Bacillus subtilis ResA, a thioredoxin-like protein involved in cytochrome c maturation PNAS, March 21, 2006; 103(12): 4410 - 4415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Bernard, S. Quevillon-Cheruel, S. Merchant, B. Guiard, and P. P. Hamel Cyc2p, a Membrane-bound Flavoprotein Involved in the Maturation of Mitochondrial c-Type Cytochromes J. Biol. Chem., December 2, 2005; 280(48): 39852 - 39859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Feissner, C. S. Beckett, J. A. Loughman, and R. G. Kranz Mutations in Cytochrome Assembly and Periplasmic Redox Pathways in Bordetella pertussis J. Bacteriol., June 15, 2005; 187(12): 3941 - 3949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hartig, H. Geng, A. Hartmann, A. Hubacek, R. Munch, R. W. Ye, D. Jahn, and M. M. Nakano Bacillus subtilis ResD Induces Expression of the Potential Regulatory Genes yclJK upon Oxygen Limitation J. Bacteriol., October 1, 2004; 186(19): 6477 - 6484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Erlendsson, M. Moller, and L. Hederstedt Bacillus subtilis StoA Is a Thiol-Disulfide Oxidoreductase Important for Spore Cortex Synthesis J. Bacteriol., September 15, 2004; 186(18): 6230 - 6238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. D. Page, P. P. Hamel, S. T. Gabilly, H. Zegzouti, J. V. Perea, J. M. Alonso, J. R. Ecker, S. M. Theg, S. K. Christensen, and S. Merchant A Homolog of Prokaryotic Thiol Disulfide Transporter CcdA Is Required for the Assembly of the Cytochrome b6f Complex in Arabidopsis Chloroplasts J. Biol. Chem., July 30, 2004; 279(31): 32474 - 32482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Crow, R. M. Acheson, N. E. Le Brun, and A. Oubrie Structural Basis of Redox-coupled Protein Substrate Selection by the Cytochrome c Biosynthesis Protein ResA J. Biol. Chem., May 28, 2004; 279(22): 23654 - 23660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. G. Bernard, S. T. Gabilly, G. Dujardin, S. Merchant, and P. P. Hamel Overlapping Specificities of the Mitochondrial Cytochrome c and c1 Heme Lyases J. Biol. Chem., December 12, 2003; 278(50): 49732 - 49742. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |