HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 278, Issue 21, 18990-18996, May 23, 2003

This Article Full Text Full Text (PDF) All Versions of this Article: 278/21/18990    most recent M300949200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stolz, J. Articles by Vielreicher, M. Search for Related Content PubMed PubMed Citation Articles by Stolz, J. Articles by Vielreicher, M. Social Bookmarking                      What's this?

Tpn1p, the Plasma Membrane Vitamin B₆ Transporter of Saccharomyces cerevisiae^*

Jürgen Stolz  and Martin Vielreicher

From the Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Universitätsstrasse 31, D-93040 Regensburg, Germany

Pyridoxine (PN) is a metabolic precursor of pyridoxal phosphate that functions as a cofactor of many enzymes in amino acid metabolism. PN, pyridoxal, and pyridoxamine are collectively referred to as vitamin B₆, and mammalian organisms depend on its uptake from the diet. In addition to the ability to use extracellular vitamin B₆, most unicellular organisms are also capable of synthesizing PN to generate pyridoxal phosphate. Here, we report the isolation of Saccharomyces cerevisiae mutants that have lost the ability to transport PN across the plasma membrane. We used these mutants to isolate TPN1, the first known gene encoding a transport protein for vitamin B₆. Tpn1p is a member of the purine-cytosine permease family within the major facilitator superfamily. The protein functions as a proton symporter, localizes to the plasma membrane, and has high affinity for PN. TPN1 mutants lost the ability to utilize extracellular PN, pyridoxal, and pyridoxamine, showing that there is no other transporter for vitamin B₆ encoded in the genome. Amino acid substitutions that led to a loss of Tpn1p function localized to transmembrane domain 4 within the 12-transmembrane domain protein. Moreover, expression of TPN1 was regulated and increased with decreasing concentrations of vitamin B₆ in the medium. We also provide evidence that of the highly conserved SNZ and SNO genes in S. cerevisiae, only the protein encoded by SNZ1 is required for vitamin B₆ biosynthesis.

Received for publication, January 28, 2003 , and in revised form, March 12, 2003.

To whom correspondence should be addressed. Tel.: 49-941-943-3005; Fax: 49-941-943-3352; E-mail: juergen.stolz{at}biologie.uni-regensburg.de.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Vogl, C. M. Klein, A. F. Batke, M. E. Schweingruber, and J. Stolz Characterization of Thi9, a Novel Thiamine (Vitamin B1) Transporter from Schizosaccharomyces pombe J. Biol. Chem., March 21, 2008; 283(12): 7379 - 7389. [Abstract] [Full Text] [PDF]

				U. Muhlenhoff, M. J. Gerl, B. Flauger, H. M. Pirner, S. Balser, N. Richhardt, R. Lill, and J. Stolz The Iron-Sulfur Cluster Proteins Isa1 and Isa2 Are Required for the Function but Not for the De Novo Synthesis of the Fe/S Clusters of Biotin Synthase in Saccharomyces cerevisiae Eukaryot. Cell, March 1, 2007; 6(3): 495 - 504. [Abstract] [Full Text] [PDF]

				S. Wagner, A. Bernhardt, J. E. Leuendorf, C. Drewke, A. Lytovchenko, N. Mujahed, C. Gurgui, W. B. Frommer, E. Leistner, A. R. Fernie, et al. Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis PLANT CELL, July 1, 2006; 18(7): 1722 - 1735. [Abstract] [Full Text] [PDF]

				H. M. Pirner and J. Stolz Biotin Sensing in Saccharomyces cerevisiae Is Mediated by a Conserved DNA Element and Requires the Activity of Biotin-Protein Ligase J. Biol. Chem., May 5, 2006; 281(18): 12381 - 12389. [Abstract] [Full Text] [PDF]

				M. Gengenbacher, T. B. Fitzpatrick, T. Raschle, K. Flicker, I. Sinning, S. Muller, P. Macheroux, I. Tews, and B. Kappes Vitamin B6 Biosynthesis by the Malaria Parasite Plasmodium falciparum: BIOCHEMICAL AND STRUCTURAL INSIGHTS J. Biol. Chem., February 10, 2006; 281(6): 3633 - 3641. [Abstract] [Full Text] [PDF]

				P. Reihl and J. Stolz The Monocarboxylate Transporter Homolog Mch5p Catalyzes Riboflavin (Vitamin B2) Uptake in Saccharomyces cerevisiae J. Biol. Chem., December 2, 2005; 280(48): 39809 - 39817. [Abstract] [Full Text] [PDF]

				C. Wrenger, M.-L. Eschbach, I. B. Muller, D. Warnecke, and R. D. Walter Analysis of the Vitamin B6 Biosynthesis Pathway in the Human Malaria Parasite Plasmodium falciparum J. Biol. Chem., February 18, 2005; 280(7): 5242 - 5248. [Abstract] [Full Text] [PDF]

				J. Stolz, H. J. P. Wohrmann, and C. Vogl Amiloride Uptake and Toxicity in Fission Yeast Are Caused by the Pyridoxine Transporter Encoded by bsu1+ (car1+) Eukaryot. Cell, February 1, 2005; 4(2): 319 - 326. [Abstract] [Full Text] [PDF]

				W. W. Hope, L. Tabernero, D. W. Denning, and M. J. Anderson Molecular Mechanisms of Primary Resistance to Flucytosine in Candida albicans Antimicrob. Agents Chemother., November 1, 2004; 48(11): 4377 - 4386. [Abstract] [Full Text] [PDF]

				C. Esser, N. Ahmadinejad, C. Wiegand, C. Rotte, F. Sebastiani, G. Gelius-Dietrich, K. Henze, E. Kretschmann, E. Richly, D. Leister, et al. A Genome Phylogeny for Mitochondria Among {alpha}-Proteobacteria and a Predominantly Eubacterial Ancestry of Yeast Nuclear Genes Mol. Biol. Evol., September 1, 2004; 21(9): 1643 - 1660. [Abstract] [Full Text] [PDF]

				B. R. Belitsky Physical and Enzymological Interaction of Bacillus subtilis Proteins Required for De Novo Pyridoxal 5'-Phosphate Biosynthesis J. Bacteriol., February 15, 2004; 186(4): 1191 - 1196. [Abstract] [Full Text] [PDF]

				T. Morita, K. Takegawa, and T. Yagi Disruption of the plr1 + Gene Encoding Pyridoxal Reductase of Schizosaccharomyces pombe J. Biochem., February 1, 2004; 135(2): 225 - 230. [Abstract] [Full Text] [PDF]

				H. M. Said, A. Ortiz, and T. Y. Ma A carrier-mediated mechanism for pyridoxine uptake by human intestinal epithelial Caco-2 cells: regulation by a PKA-mediated pathway Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1219 - C1225. [Abstract] [Full Text] [PDF]