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J. Biol. Chem., Vol. 278, Issue 21, 18997-19007, May 23, 2003

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Mutations in Tau Gene Exon 10 Associated with FTDP-17 Alter the Activity of an Exonic Splicing Enhancer to Interact with Tra2^*

Zhihong Jiang , Hao Tang , Necat Havlioglu  , Xiaochun Zhang , Stefan Stamm ¶, Riqiang Yan || and Jane Y. Wu  **

From the Departments of Pediatrics and of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, ^¶ Universitate Erlangen-Nurenberg, Institute of Biochemistry, Fahrstrasse 17, Erlangen 91054, Germany, ^|| Department of Cell and Molecular Biology, Pharmacia Corp., Kalamazoo, Michigan 49007

Mutations in the human tau gene leading to aberrant splicing have been identified in FTDP-17, an autosomal dominant hereditary neurodegenerative disorder. Molecular mechanisms by which such mutations cause tau aberrant splicing were not understood. We characterized two mutations in exon 10 of the tau gene, N279K and Del280K. Our results revealed an exonic splicing enhancer element located in exon 10. The activity of this AG-rich splicing enhancer was altered by N279K and Del280K mutations. This exonic enhancer element interacts with human Tra2 protein. The interaction between Tra2 and the exonic splicing enhancer correlates with the activity of this enhancer element in stimulating splicing. Biochemical studies including in vitro splicing and RNA interference experiments in transfected cells support a role for Tra2 protein in regulating alternative splicing of human tau gene. Our results implicate the human tau gene as a target gene for the alternative splicing regulator Tra2, suggesting that Tra2 may play a role in aberrant tau exon 10 alternative splicing and in the pathogenesis of tauopathies.

Received for publication, February 20, 2003 , and in revised form, March 18, 2003.

^** To whom correspondence should be addressed. Tel.: 314-286-2798; Fax: 314-286-2892; E-mail: jwu{at}molecool.wustl.edu.

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